Email this article THE COMPARATIVE STUDY OF HUMAN ENDOGENOUS RETROVIRUS HERV-E λ 4-1 EXPRESSION IN BLOOD IMMUNE CELLS OF MULTIPLE SCLEROSIS PATIENTS
- Authors: Goldina IA1, Markova EV1,2
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Affiliations:
- Laboratory of Neuroimmunology, Federal State Budgetary Scientific Research Institute of the Fundamental and Clinical Immunology, Novosibirsk
- Federal State Budgetary Educational Institution of Higher Professional Education “Novosibirsk State Pedagogical University”, Novosibirsk
- Issue: Vol 19, No 1S (2019)
- Pages: 78-80
- Section: Articles
- Published: 15.12.2019
- URL: https://journals.eco-vector.com/MAJ/article/view/19334
- ID: 19334
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Abstract
In order to investigate the function of human endogenous retrovirus HERV-E λ 4-1 in multiple sclerosis pathogenesis, the comparative research of frequency of this retrovirus expression and mRNA level on different types of blood immune cells of progredient course multiple sclerosis patients have been conducted with using of the reverse - transcriptase polymerase chain reaction method. Peripheral blood mononuclear cells were isolated on Ficoll density gradient centrifugations. Monocytes were separated by the adhesion to plastic Petri dishes. For the estimation of the mitogen-induced HERV-E λ 4-1 expression, blood mononuclear cells were incubated with adding of phytogemagglutinin or pokeweed mitogen during 72 hours in CO2 incubator at 37 °C and 5% CO2. Results of HERV-E λ 4-1 env gene expression estimation demonstrate that both monocytes and lymphocytes express HERV-E λ 4-1. The level of the HERV-E λ 4-1 env mRNA was higher in lymphocytes than in monocytes. The main source of HERV-E λ 4-1 in progredient course MS patients between blood immune cells are lymphocytes, especially B lymphocytes.
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About the authors
I A Goldina
Laboratory of Neuroimmunology, Federal State Budgetary Scientific Research Institute of the Fundamental and Clinical Immunology, Novosibirsk
E V Markova
Laboratory of Neuroimmunology, Federal State Budgetary Scientific Research Institute of the Fundamental and Clinical Immunology, Novosibirsk; Federal State Budgetary Educational Institution of Higher Professional Education “Novosibirsk State Pedagogical University”, Novosibirsk
References
- Goldina IA, Markova EV. Neiroimmunnye mehanismy patogeneza rasseyannogo sclerosa. Krasnoyarsk: Nauchno-innovacionnyi centr; 2018. 150 p. (In Russ.). https://doi.org/10.12731/978-5-906314-89-5.
- Lander ES, Linton IM, Birren B, et al. Initial sequencing and analysis of the human genome. Nature. 2001;409:860-921. https://doi.org/10.1038/35057062.
- Smagin AA, Goldina IA, Gaidul KV. Issledovanie proliferativnoi aktivnosti mononuclearnyh kletok krovi bolnyh rasseyannym sklerozom pri vozdeistvii peptide regiona envelope endogennogo retrovirusa cheloveka HERV-E λ 4-1. Medicinskaya immunologia. 2014;16(3):247-256. (In Russ.).
- Vargu L, Rodrigues-Tome P, Sperber GO, et al. Classification and characterization of human endogenous retroviruses; mosaic forms are common. Retrovirology. 2016;13:7. https://doi.org/10.1186/s12977-015-0232-y.
- Laufer G, Mayer J, Mueller BF, et al. Analysis of transcribed human endogenous retrovirus W env loci clarifies the origin of multiple-sclerosis associated retrovirus env sequences. Retrovirology. 2009;6:37. https://doi.org/10.1186/1742-4690-6-37.
- Goldina IA, Gaidul KV, Kozlov VA. UFO-inducyrovannaia ekspressia endogennogo retrovirusa cheloveka HERV-E λ 4-1 v mononuclearnyh kletkah krovi. Bulleten sibirskoi mediciny. 2018;17(1):36-44. (In Russ.). https://doi.org/10.20538/1682-0363-2018-1-36-44.
- Goldina IA, Safronova IV, Goldin BG, Gaidul KV. Pattern of relationship of the level of mRNA of human endogenous retrovirus of class I L 4-1 envelope gene and proliferative activity of blood mononuclear cells. International Immunology. 2010;22(S1):i71.
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