PRESERVED NUMBER OF OREXIN NEURONS IN POSTMORTEM HYPOTHALAMIC TISSUE OF CHRONIC ALCOHOLICS
- Authors: Kudriavova AS1, Meskenaite V2, Mikhailov VI3, Schesny M2, Korneva EA1, Gavrilov YV1, Valko PO2
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Affiliations:
- Institute of Experimental Medicine, Saint Petersburg
- University Hospital Zurich
- Alexandrovskaya City Hospital, Saint Petersburg
- Issue: Vol 19, No 1S (2019)
- Pages: 91-92
- Section: Articles
- Published: 15.12.2019
- URL: https://journals.eco-vector.com/MAJ/article/view/19341
- ID: 19341
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Introduction. It is well established that orexin system is strongly related to addiction to drugs and especially alcohol. Several studies reported that orexin neurons in lateral hypothalamus may be involved in reward and motivation [1]. Schneider et al. showed that microinjection of orexin A into the lateral hypothalamus or paraventricular nucleus of the hypothalamus increased alcohol intake in rats [3]. The numbers of orexin neurons were found to be increased in opiate addiction in humans [4] and cocaine addiction in rats [2], but the integrity of orexin neurons has not yet been studied in human alcoholics. Considering these data, we wondered whether long-term alcohol use would affect the number of orexin neurons in the hypothalamus in humans. Material and methods. We studied the effect of alcohol addiction on brain orexin neurons on postmortem human hypothalamic tissue of alcoholics and non-alcoholics (control subjects). Diagnoses were based on hospital records, available for all 19 cases. All control subjects had no clinical evidence of any neurological or psychiatric disorder. Subjects having disorders or medications affecting CNS, history of drug abuse were excluded from the study. We immunostained hypothalamic sections for orexin A (hypocretin-1) as previously described [5]. The total number of positively labeled orexin A neurons was estmated using a Zeiss (Oberkochen, Germany) Axiophot microscope with a motorized stage interfaced with a Stereo Investigator system (MBF Biosciences, Willston, VT). Results and discussion. We found no significant difference between alcoholics and control subjects. Specifically, the total number of orexin A neurons in the alcoholic patients was more (9%) than in control subjects (123087 ± 18536 and 110431 ± 14439, respectively, with p = 0.11). We found that the number of orexin neurons in lateral hypothalamus in alcoholic patients is no statistically different from the number in non-alcoholic subjects. Even not considering the increased p-value, the difference between alcoholics and non-alcoholics remains very small (> 10%), that is surprising because theoretically we could expect the number of orexin neurons be reduced due to neurotoxic effects of alcohol or increased due to their involvement in addiction and neuroplastic effects. This finding allows us to assume that the orexin system is stronger involved in opiate/cocaine addiction than alcohol addiction. However, our work continues, and with additional research, we could find out more about the integrity of the orexin neurons in alcoholism.About the authors
A S Kudriavova
Institute of Experimental Medicine, Saint Petersburg
V Meskenaite
University Hospital Zurich
V I Mikhailov
Alexandrovskaya City Hospital, Saint Petersburg
M Schesny
University Hospital Zurich
E A Korneva
Institute of Experimental Medicine, Saint Petersburg
Y V Gavrilov
Institute of Experimental Medicine, Saint Petersburg
P O Valko
University Hospital Zurich
References
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