ANTIMICROBIAL ACTIVITY OF LYSOZYME TOWARDS LISTERIA MONOCYTOGENES AT VARIOUS MEDIUM CONDITIONS

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Abstract

The in vitro antimicrobial action of human leukocyte lysozyme from on gram-positive bacterium Listeria monocytogenes under various medium conditions was studied. It was shown that in a low ionic strength buffer (without NaCl), lower doses of lysozyme are required to reveal the microbicidal effect than in the case of 0.075 or 0.15 M NaCl. The bacterial growth phase does not significantly affect the antimicrobial activity of lysozyme. The results obtained are consistent with the two-stage mechanism of the antimicrobial action of lysozyme, which includes enzymatic and non-enzymatic action.

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M N Berlov

Institute of Experimental Medicine, Saint Petersburg

S V Legkovoy

Saint Petersburg State University, Saint Petersburg

E S Umnyakova

Institute of Experimental Medicine, Saint Petersburg

V N Kokryakov

Institute of Experimental Medicine, Saint Petersburg; Saint Petersburg State University, Saint Petersburg

References

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Copyright (c) 2019 Berlov M.N., Legkovoy S.V., Umnyakova E.S., Kokryakov V.N.

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