Experimental evaluation of the effect of beta-D-glucan on the survival of irradiated mice

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Abstract

The relevance is determined by the need to develop new means of antiradiation protection, which could be used for irradiating people in case of emergency situations or for medical use of ionizing radiation for diagnostic or therapeutic purposes.

Aim: to evaluate the prospects of beta-D-glucan as a candidate drug for the development of a pharmacological means to reduce the toxic effects of radiation exposure.

Materials and methods. In experiments on male mice the protective effect of beta-D-glucan derived from Pleurotus ostreatus mushroom on the parameters of 30-day survival of irradiated rodents exposed to lethal doses of X-ray radiation was studied. Beta-D-glucan was administered intragastrically in different doses in preventive or therapeutic regimens.

Results. It was shown that intragastric administration of beta-D-glucan at a dose of 500 mg/kg 1 h after 7.5 Gy X-Ray irradiation protected from the death of irradiated mice. There was a 27% increase in the 30-day survival rate of mice compared to the control group (47% and 20%, respectively). This dose of the drug also increased the 30-day survival rate of mice by 26% when administered 0.5 h before or 2 hours after 8 Gy irradiation. The good tolerance of intragastric administration of beta-D-glucan in mice at a dose of 500 mg/kg was shown. There were no negative effects of the drug during 3 weeks of follow-up.

Conclusion. The results can indicate that beta-D-glucan derived from Pleurotus ostreatus has an antiradiation potential in the oral route of administration, having a protective effect on the survival of lethally irradiated animals, and shows the properties of a radiomitigator and radioprotector, but the identified effect requires further study.

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About the authors

Elena V. Murzina

S.M. Kirov Military Medical Academy of the Ministry of Defense of Russian Federation; Institute of Experimental Medicine

Author for correspondence.
Email: elenmurzina@mail.ru
ORCID iD: 0000-0001-7052-3665
SPIN-code: 5188-0797

PhD in Biology, senior researcher of Research Laboratory of Medicinal and Environmental Toxicology, Research Center

Russian Federation, Saint Petersburg

Genrikh A. Sofronov

S.M. Kirov Military Medical Academy of the Ministry of Defense of Russian Federation; Institute of Experimental Medicine

Email: gasofronov@mail.ru
ORCID iD: 0000-0002-8587-1328
SPIN-code: 7334-4881
Scopus Author ID: 7003953555
ResearcherId: G-4791-2015

MD, PhD, Professor, Member of the RAS, Head of the Research Laboratory of Medicinal and Environmental Toxicology, Research Center

Russian Federation, Saint Petersburg

Andrei S. Simbirtsev

Institute of Experimental Medicine

Email: simbirtsev@hpb-spb.com
ORCID iD: 0000-0002-8228-4240
SPIN-code: 2064-7584
Scopus Author ID: 7003758888
ResearcherId: K-5061-2014

PhD in Biology, Professor, Corresponding Member RAS, Head of the Department of Medical Biotechnology and Immunopharmacology

Russian Federation, Saint Petersburg

Natalia V. Aksenova

S.M. Kirov Military Medical Academy of the Ministry of Defense of Russian Federation

Email: vmeda-nio@mil.ru
SPIN-code: 6821-6887

MD, PhD, Research Fellow of Research Department (All-Army Register of the Ministry of Defense of the Russian Federation)

Russian Federation, Saint Petersburg

Olga M. Veselova

S.M. Kirov Military Medical Academy of the Ministry of Defense of Russian Federation

Email: vmeda-nio@mil.ru

research fellow of Research Laboratory of Medicinal and Environmental Toxicology, Research Center

Russian Federation, Saint Petersburg

Aleksandr V. Zavirskiy

S.M. Kirov Military Medical Academy of the Ministry of Defense of Russian Federation

Email: vtmz@vmeda.org
SPIN-code: 3935-3233

PhD student of the Department of Military Toxicology and Medical Protection

Russian Federation, Saint Petersburg

Tat’yana G. Krylova

S.M. Kirov Military Medical Academy of the Ministry of Defense of Russian Federation

Email: vmeda-nio@mil.ru
SPIN-code: 5188-0797

PhD in Biology, junior researcher of Research Laboratory of Medicinal and Environmental Toxicology, Research Center

Russian Federation, Saint Petersburg

Mark M. Shamtsyan

Saint Petersburg State Institute of Technology

Email: mark.shamtsyan@yandex.ru
SPIN-code: 2609-1909

PhD, Associate Professor of Department of Technology of Microbiological Synthesis

Russian Federation, Saint Petersburg

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2. Fig. 1. Survival curves of mice after a total 7.5 Gy X-rays (K) and intragastric administration of beta-D-glucan (BG) in doses of 50, 250 or 500 mg/kg: а — using beta-D-glucan 1 h before irradiation; b — using beta-D-glucan 1 h after irradiation

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3. Fig. 2. 30-day survival rate of mice exposed to X-ray radiation at a dose of 8 Gy and intragastric administration of beta-D-glucan (BG) in doses of 250, 500 or 1000 mg/kg

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4. Fig. 3. Weight coefficients of organs of intact mice (K) or 21 days after a single intragastric administration of beta-D-glucan (BG, 500 mg/kg)

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5. Fig. 1. Survival curves of mice after a total 7.5 Gy X-rays (K) and intragastric administration of beta-D-glucan (BG) in doses of 50, 250 or 500 mg/kg: а — using beta-D-glucan 1 h before irradiation; b — using beta-D-glucan 1 h after irradiation

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6. Fig. 2. 30-day survival rate of mice exposed to X-ray radiation at a dose of 8 Gy and intragastric administration of beta-D-glucan (BG) in doses of 250, 500 or 1000 mg/kg

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7. Fig. 3. Weight coefficients of organs of intact mice (K) or 21 days after a single intragastric administration of beta-D-glucan (BG, 500 mg/kg)

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Copyright (c) 2020 Murzina E.V., Sofronov G.A., Simbirtsev A.S., Aksenova N.V., Veselova O.M., Zavirskiy A.V., Krylova T.G., Shamtsyan M.M.

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