Polymorphisms of genes of interleukin-6 and alpha-1 chain of collagen type 1 in postmenopausal women with knee osteoarthritis
- Authors: Ignatenko G.A.1, Reznichenko N.A.2, Fedulichev P.N.2, Maylyan E.A.1, Kharaeva Z.F.3
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Affiliations:
- M. Gorky Donetsk State Medical University
- V.I. Vernadsky Crimean Federal University
- Kabardino-Balkarian State University named after Berbekov
- Issue: Vol 23, No 3 (2023)
- Pages: 31-40
- Section: Original research
- URL: https://journals.eco-vector.com/MAJ/article/view/375358
- DOI: https://doi.org/10.17816/MAJ375358
- ID: 375358
Cite item
Abstract
BACKGROUND: To date in the Russian Federation insufficient attention has been paid to the study of IL6 and COL1A1 gene polymorphisms role in the development of knee osteoarthritis. And the results of the single carried out to date studies, devoted to the research of polymorphic variants of the above genes influence on the osteoarthritis development, are insufficient for substantiated conclusions.
AIM: To study the frequency of alleles and genotypes of the IL6 gene rs1800795 polymorphism and COL1A1 gene rs1107946 and rs1800012 polymorphisms in postmenopausal women with knee osteoarthritis.
MATERIALS AND METHODS: The results of 157 postmenopausal women survey with knee osteoarthritis were selected and analyzed. The control group consisted of 326 women of the same age without signs of joint disease. The study of polymorphisms rs1800795, rs1107946 and rs1800012 was performed by real-time polymerase chain reaction.
RESULTS: The conducted studies showed that in the general group of examined women the frequency of all three studied polymorphisms genotypes registration corresponded to the Hardy-Weinberg law. An uneven (p = 0.043) distribution of rs1800795 polymorphism genotypes was found in the group of women with osteoarthritis and in the control group in the study of the IL6 gene polymorphic variants frequency detection. This difference was due to more frequent GG genotype registration of the above polymorphism (odds ratio = 1.75; 95% confidence interval: 1.12–2.72; p = 0.021) among women with knee osteoarthritis. Associations of rs1107946 and rs1800012 COL1A1 gene polymorphisms were not found (p > 0.05).
CONCLUSIONS: An association between GG genotype of the IL6 gene rs1800795 polymorphism and knee osteoarthritis in postmenopausal women has been established. Genotypes and alleles of COL1A1 gene rs1107946 and rs1800012 polymorphisms were not associated with joint disease.
Keywords
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About the authors
Grigory A. Ignatenko
M. Gorky Donetsk State Medical University
Email: prop-vnutr-medicina@dnmu.ru
ORCID iD: 0000-0003-3611-1186
SPIN-code: 3893-0662
MD, Dr. Sci. (Med.), Professor, Corresponding Member of the National Academy of Medical Sciences of Ukraine, Head of the Department of Propaedeutics of Internal Diseases
Russian Federation, DonetskNatalia A. Reznichenko
V.I. Vernadsky Crimean Federal University
Email: professorreznichenko@mail.ru
ORCID iD: 0000-0003-3396-1046
SPIN-code: 9740-2247
MD, Dr. Sci. (Med.), Professor, Professor of the Department of Obstetrics and Gynecology No. 1, Medical Academy named after S.I. Georgievsky
Russian Federation, SimferopolPavel N. Fedulichev
V.I. Vernadsky Crimean Federal University
Email: pfedulichev@yandex.ru
ORCID iD: 0000-0002-5492-0270
SPIN-code: 7364-3067
MD, Cand. Sci. (Med.), Assistant Professor, Assistant Professor of the Department of Topographic Anatomy, Medical Academy named after S.I. Georgievsky
Russian Federation, SimferopolEduard A. Maylyan
M. Gorky Donetsk State Medical University
Email: maylyan.ea@yandex.com
ORCID iD: 0000-0003-2845-7750
SPIN-code: 5420-2760
MD, Dr. Sci. (Med.), Professor, Head of the Department of Microbiology, Virology, Immunology and Allergology
Russian Federation, DonetskZaira F. Kharaeva
Kabardino-Balkarian State University named after Berbekov
Author for correspondence.
Email: irafe@yandex.ru
ORCID iD: 0000-0003-2302-2491
SPIN-code: 3563-7649
MD, Dr. Sci. (Med.), Professor, Head of the Department of Microbiology, Virology and Immunology
Russian Federation, NalchikReferences
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