Analysis of methods for modeling deep defects of the skin and articular cartilage on laboratory animals in the experiment

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Abstract

The creation and implementation of new methods and means of local treatment of wounds occurs in stages, however, a number of difficulties arise at each stage. The article discusses one of the main problems that arise at the preclinical stage when modeling deep defects of the skin and articular cartilage – the difficulty of accurately reproducing full-layer defects of the skin and articular tissue. Various factors influencing the success of such modeling also investigated, including the type of animal, the size of the defect and its location.

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About the authors

Daulet D. Izbulatov

Military Innovative Technopolis “ERA”

Email: izbulatov98@mail.ru

senior operator of 3rd scientific company

Russian Federation, Anapa

Natalia V. Varlamova

Military Innovative Technopolis “ERA”

Email: varlamova@tpu.ru
ORCID iD: 0000-0002-6100-2427
SPIN-code: 9139-6019

Cand. Sci. (Technol.), Senior Research Associate

Russian Federation, Anapa

Vladimir E. Mikhailov

Military Innovative Technopolis “ERA”

Email: mikhaylov.ve@yandex.ru

senior operator of 3rd scientific company

Russian Federation, Anapa

Ilya V. Markin

Military Innovative Technopolis “ERA”

Email: ilya.markin.92@bk.ru
ORCID iD: 0000-0002-9334-910X
SPIN-code: 6021-7645

Cand. Sci. (Technol.), Senior Research Associate

Russian Federation, Anapa

Artur L. Erdniev

Military Innovative Technopolis “ERA”

Email: arti-erd@mail.ru

senior operator of 3rd scientific company

Russian Federation, Anapa

Petr K. Potapov

Military Innovative Technopolis “ERA”

Email: forwardspb@mail.ru
SPIN-code: 5979-4490

MD, Cand. Sci. (Med.), Deputy Head of Department of Biomedical Research

Russian Federation, Anapa

Yurii A. Utkin

Ural Research Institute of Composite Materials

Author for correspondence.
Email: uautkin@yandex.ru

Deputy General Director for Medical Direction

Russian Federation, Perm

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Supplementary files

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2. Fig. 1. Macroscopic characteristics of skin defects: a — infliction of a skin wound on a rat; b — rat skin wound model after surgery; с — skin wounds treated with prepared medicine after 2 days

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3. Fig. 2. Macroscopic characteristics of skin defects: а — skin wound model in diabetic mice; b — wound model in smooth-haired guinea pigs; c — full-thickness dorsal wound in diabetic rats; d — planar wound in rats

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4. Fig. 3. Defects in the surface of the joint: a — full-layer defect of the loaded surface of the knee joint of a sheep; b — defect of the right knee joint of a white rabbit; с — computed tomography of the intact rabbit knee joint; d — computed tomography of the joint after chondroplasty (2 round defects on the patellar surface of the femur are well visualized)

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5. Fig. 4. Defects in the surface of the joint: а — the area of surgical intervention after the formation of a full-thickness perforated defect on the patellar surface of the femoral condyle; b — the process of making cartilage defects; с — the process of making cartilage microfractures

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