Influence of various light regimes on some circadian rhythms of transplantable melanoma B16.



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Abstract

Actuality. Today it is known that disturbance of the lighting regime, expressed both by lengthening of the light period and its reduction, can not only affect the regulation of circadian rhythms of the organism, but also contributes to the initiation of neoplasm growth.

The aim of the study was to investigate circadian rhythmicity of melatonin level, some micromorphometric indices ща tumor cells and expression of genes Bmal1, Clock and Per2 in them in mice with transplanted melanoma B16.

Materials and Methods. The study was conducted on 75 mice with subcutaneously transplanted melanoma B16, divided into 3 groups: control group, in which animals were kept under fixed light regime (light/darkness 10/14 hours with light on at 8:00 and off at 18:00), group under dark deprivation conditions, with animals kept under constant light 24 hours a day and group, in which animals were kept in constant darkness. The duration of the experiment was 2 weeks.

Results. It was shown that under conditions of fixed light there are reliable circadian rhythms (CR) for all studied parameters, except for the nuclear-cytoplasmic ratio, the CR of which was not revealed in any group. Constant darkness leads to rearrangement of all identified rhythms, and constant light causes destruction of all CRs except the Clock expression rhythm.

Conclusion. This study shows that light disturbances, whether constant light or constant darkness, lead to significant changes in the structure of the studied circadian rhythms.

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About the authors

David A Areshidze

AVTSYN RESEARCH INSTITUTE OF HUMAN MORPHOLOGY OF FEDERAL STATE BUDGETARY SCIENTIFIC INSTITUTION "PETROVSKY NATIONAL RESEARCH CENTRE OF SURGERY"

Author for correspondence.
Email: labcelpat@mail.ru
ORCID iD: 0000-0003-3006-6281

David A. Areshidze – Cand. Sci. (Biol.), Leading Research Associate, Head of Laboratory of Pathology of Cell of the Federal State Budgetary Institution «A.P. Avtsyn Research Institute of Human Morphology»,

Russian Federation

Maria A Kozlova

Avtsyn Research Institute of Human Morphology of Federal state budgetary scientific institution «Petrovsky National Research Centre of Surgery», Moscow, Russia.

Email: ma.kozlova2021@outlook.com
ORCID iD: 0000-0001-6251-2560
SPIN-code: 5647-1372

канд. биол. наук, старший научный сотрудник лаборатории патологии клетки,

Russian Federation, Russian Federation, 117418, Moscow, Tsyurupy st., 3

Denis V Mishchenko

Federal state budgetary scientific institution Federal Research Center of Problems of Chemical Physics and Medicinal Chemistry Russian Academy of Sciences, Chernogolovka, Russia.

Email: mdv@icp.ac.ru
ORCID iD: 0000-0003-3779-3211
SPIN-code: 4213-3318

Dr. Sci. (Biol.), Leading Researcher Experimental Tumor Chemotherapy Group

142432, Russian Federation, Moscow region, Chernogolovka, 1, Academician Semenov Ave.

Valery P Chernikov

Avtsyn Research Institute of Human Morphology of Federal state budgetary scientific institution «Petrovsky National Research Centre of Surgery», Moscow, Russia.

Email: 1200555@mail.ru
ORCID iD: 0000-0002-3253-6729
SPIN-code: 3125-7837

Dr. Sci. (Med.), Leading Researcher of laboratory of Cell Pathology

Russian Federation, Russian Federation, 117418, Moscow, Tsyurupy st., 3

Tatyana V Bezuglova

Avtsyn Research Institute of Human Morphology of Federal state budgetary scientific institution «Petrovsky National Research Centre of Surgery», Moscow, Russia.

Email: bezuglovat@mail.ru
ORCID iD: 0000-0001-7792-1594
SPIN-code: 3943-4400

Dr. Sci. (Biol.), Deputy Director for Scientific Work

Russian Federation, Russian Federation, 117418, Moscow, Tsyurupy st., 3

Maxim V Mnikhovich

Avtsyn Research Institute of Human Morphology of Federal state budgetary scientific institution «Petrovsky National Research Centre of Surgery», Moscow, Russia.

Email: mnichmaxim@yandex.ru
ORCID iD: 0000-0001-7147-7912
SPIN-code: 6975-6677

Dr. Sci. (Med.), Leading Researcher of the central pathology laboratory

Russian Federation, Russian Federation, 117418, Moscow, Tsyurupy st., 3

Zarina V Gioeva

Avtsyn Research Institute of Human Morphology of Federal state budgetary scientific institution «Petrovsky National Research Centre of Surgery», Moscow, Russia.

Email: bezuglovat@mail.ru
ORCID iD: 0000-0002-5456-8692
SPIN-code: 9210-9726

Dr. Sci. (Med.), head of the central pathology laboratory

Russian Federation, Russian Federation, 117418, Moscow, Tsyurupy st., 3

Ugulzhan Yuldashovna Allayarova

Federal state budgetary scientific institution Federal Research Center of Problems of Chemical Physics and Medicinal Chemistry Russian Academy of Sciences, Chernogolovka, Russia

Email: bezuglovat@mail.ru
ORCID iD: 0000-0002-8264-0997
SPIN-code: 4996-3473

junior researcher Experimental Tumor Chemotherapy Group

Russian Federation, 142432, Russian Federation, Moscow region, Chernogolovka, 1, Academician Semenov Ave.

Anna I Anurkina

Avtsyn Research Institute of Human Morphology of Federal state budgetary scientific institution «Petrovsky National Research Centre of Surgery», Moscow, Russia.

Email: anyaaai1925@gmail.com
ORCID iD: 0009-0003-0011-1114
SPIN-code: 9812-3412

research assistant of laboratory of Cell Pathology

Russian Federation, 117418, Moscow, Tsyurupy st., 3

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