Changes in the liver of Djungarian hamsters under conditions of a three-month supply of water-soluble silicon of various concentrations

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Abstract

BACKGROUND: Silicon enters the human body through drinking water, air and food. Silicon nanoparticles used in the cosmetic, pharmaceutical and food industries are known to have biological activity. Taking into account the widespread prevalence of silicon compounds, the issue of the safety of its use is becoming more urgent.

AIM: To study the effect of water-soluble silicon on the morphological structure of the liver of Djungarian hamsters for three months.

MATERIALS AND METHODS: The experiment was carried out on Djungarian hamsters kept in normal vivarium conditions under natural light. The animals were divided into three groups: control, which received bottled drinking water; the first experimental group, which received the same water, but with the addition of sodium metasilicate nine-hydrate at a concentration of 10 mg/l in terms of silicon; the second experimental group, which also received the same water, but with the concentration of sodium metasilicate nine-hydrate doubled (up to 20 mg/l). After three months, the animals were removed from the experiment. Sections were processed by general histological (hematoxylin and eosin, Van Gieson method, toluidine blue), histochemical (monoamine oxidase-positive cells) methods.

RESULTS: In the liver of hamsters from the experimental groups, changes in the micromorphological structure were revealed, such as an increase in the nuclear area, nuclear-cytoplasmic ratio of hepatocytes, and the diameter of sinusoidal capillaries. Moreover, more pronounced changes were observed in the liver of hamsters of the second experimental group, such as polymorphic cell infiltration of the portal tracts, an increase in the number of eosinophils, deformation of hepatocyte nuclei and the appearance of apoptotic bodies. A decrease in the area of mast cells and an increase in their number, as well as the number of monoamine oxidase-positive cells in the liver of hamsters of both experimental groups were recorded.

CONCLUSIONS: An increase in the concentration of silicon supplied with drinking water in both cases is reflected in the micromorphological structure of the liver of hamsters. Moreover these changes are more pronounced in the liver of hamsters of the second experimental group.

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About the authors

Evgeniia A. Grigoreva

I.N. Ulyanov Chuvash State University

Email: shgrev@yandex.ru
ORCID iD: 0000-0003-3626-2750
SPIN-code: 9971-5435

Assistant of the Department of Medical Biology with a course of Microbiology and Virology

Russian Federation, 15 Moskovsky Ave., Cheboksary, 428015, Chuvash Republic

Valentina S. Gordova

Immanuel Kant Baltic Federal University

Email: crataegi@rambler.ru
ORCID iD: 0000-0001-5109-9862
SPIN-code: 2527-1634

MD, Cand. Sci. (Medicine), Assistante Professor, Department of Fundamental Medicine of the Higher School of Medicine of Institute of Medicine and Life Sciences

Russian Federation, 236041, Kaliningrad, st. A. Nevsky, 14

Valentina E. Sergeeva

I.N. Ulyanov Chuvash State University

Email: kafedra-biology@yandex.ru
ORCID iD: 0000-0003-3471-5226
SPIN-code: 9827-3454

Dr. Sci. (Biology), Professor, Department of Medical Biology with a course of Microbiology and Virology

Russian Federation, 15 Moskovsky Ave., Cheboksary, 428015, Chuvash Republic

Roman D. Mikheikin

I.N. Ulyanov Chuvash State University

Email: mikheykin2002@mail.ru
ORCID iD: 0009-0002-6731-7224

4rd year Student, Medicine Faculty

Russian Federation, 15 Moskovsky Ave., Cheboksary, 428015, Chuvash Republic

Valeriia S. Dedikina

I.N. Ulyanov Chuvash State University

Email: valary0d@gmail.com
ORCID iD: 0009-0005-5045-4291

6th year Student, Medicine Faculty

Russian Federation, 15 Moskovsky Ave., Cheboksary, 428015, Chuvash Republic

Darya A. Braun

Immanuel Kant Baltic Federal University

Author for correspondence.
Email: dashabraun1612@gmail.com
ORCID iD: 0009-0001-1809-1161

5th year Student, Graduate School of Medicine

Russian Federation, 236041, Kaliningrad, st. A. Nevsky, 14

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2. Fig. 1. Liver of hamsters of the control (a, d), first experimental (b, e) and second experimental (c, f) groups. Hematoxylin-eosin staining: a, b, c — ×10, d, e, f — ×1000. White arrow indicate Councilman’s bodies, black arrows indicate eosinophils

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3. Fig. 2. Liver of hamsters of the control (a), first experimental (b) and second (c) experimental groups. Staining with Van Gieson, ×400

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