Changes in progesterone and gonadoliberin levels after introduction of exogenous kisspeptin in Danio rerio females

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Abstract

BACKGROUND: According to modern concepts, the kisspeptin signaling system is the upper (central) link in the regulation of reproductive function. The neuropeptide kisspeptin is considered an indicator of a number of obstetric pathologies in humans. Therefore, it should be considered important to search for new synthetic analogues of natural kisspeptins as pharmacological agents of reproduction regulation. On the other hand, it should also be considered important to search for new effective model organisms in which certain effects manifest themselves most prominently compared to traditional experimental animals.

AIM: To quantify the effect of kisspeptin-14 on the level of gonadoliberin and progesterone in two doses, at the time typical for the action of neuropeptides, on the model organism Danio rerio.

METHODS: In this study, an analogue of kisspeptin, conventionally designated kisspeptin-14, was used. All Danio rerio females had reached puberty by the time of the experiment (6–8 months). Kisspeptin was administered in doses of 1 and 4 ng. The groups were analyzed 1 and 4 hours after administration. Before administration of the preparations, the fish were subjected to lidocaine anesthesia at a concentration of 40 mg/l for 5–6 minutes, then the drug was administered intracerebroventrically. After waiting for 1 or 4 hours, the material was collected (gonads and midbrain). Gonad and midbrain samples were homogenized, homogenates were suspended, and enzyme immunoassay was performed to determine hormone concentrations.

RESULTS: Administration of kisspeptin-14 at a dose of 1 ng led to an increase in gonadoliberin levels 4 hours after administration. With a shorter time after administration, no statistically significant results were found. Administration of kisspeptin-14 at doses of 1 and 4 ng led to an increase in progesterone levels in the gonads of Danio rerio females 1 and 4 hours after administration. At the same time, statistically significant differences compared with the control group were noted at different sample collection dates.

CONCLUSION: Intracerebroventricular administration of the kisspeptin-14 at a dose of 1 ng after 4 hours causes an increase in gonadoliberin in the structures of the midbrain. Intracerebroventricular administration of kisspeptin-14 causes an increase in progesterone in the gonads of Danio rerio females. There was no unambiguous relationship between changes in the concentration of gonadoliberin in the structures of the midbrain and changes in the concentration of progesterone in the gonads of females.

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About the authors

Alexandra A. Blazhenko

Ott Research Institute of Obstetrics, Gynecology and Reproductology; Institute of Experimental Medicine

Email: alexandrablazhenko@gmail.com
ORCID iD: 0000-0002-8079-0991
SPIN-code: 8762-3604

Researcher; Junior Researcher at the Anichkov Department of Neuropharmacology

Russian Federation, Saint Petersburg; Saint Petersburg

Marina I. Kostina

Ott Research Institute of Obstetrics, Gynecology and Reproductology; Peter the Great Saint Petersburg Polytechnic University

Author for correspondence.
Email: koctena@list.ru
ORCID iD: 0009-0005-1060-5489

Student; Junior Researcher

Russian Federation, Saint Petersburg; Saint Petersburg

Alina A. Nuzhnova

Ott Research Institute of Obstetrics, Gynecology and Reproductology; Peter the Great Saint Petersburg Polytechnic University

Email: lin.panaiotis@yandex.ru
ORCID iD: 0009-0002-1607-1471

Student; Junior Researcher

Russian Federation, Saint Petersburg; Saint Petersburg

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2. Fig. 1. Progesterone concentration in the gonads of Danio rerio in pg/mcg of total protein after administration of kisspeptin-14: 1, control group 1 hour; 2, control group 4 hours; 3, 1 hour after administration of kisspeptin-14 at a dose of 1 ng; 4, 1 hour after administration of kisspeptin-14 at a dose of 4 ng; 5, 4 hours after administration of kisspeptin-14 with a dose of 1 ng; 6, 4 hours after administration of kisspeptin-14 with a dose of 4 ng; 7, combined control group; 8, combined group with a dose of 1 ng; 9, combined group with a dose of 4 ng; 10, combined experimental the group with a time of 1 hour; 11, is a combined experimental group with a time of 4 hours. The graph shows all the individual values and the average of these data. * p < 0,05; ** p < 0,01; *** p < 0,001; **** p < 0,0001.

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3. Fig. 2. The concentration of gonadoliberin in the middle brain of Danio rerio in pg/mcg of total protein after administration of kisspeptin-14: 1, control group 1 hour; 2, control group, 4 hours; 3, 1 hour after administration of the drug with a dose of 1 ng; 4, 1 hour after administration of kisspeptin-14 with a dose of 4 ng; 5, 4 hours after administration of kisspeptin-14 with a dose of 1 ng; 6, 4 hours after administration of kisspeptin-14 with a dose of 4 ng; 7, combined control group; 8, combined group with a dose of 1 ng; 9, combined group with a dose of 4 ng; 10, combined group with time 1 hour; 11, a combined group with a time of 4 hours. The graph shows all the individual values and the average ± standard deviation according to these data. ** p < 0,01.

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