Email this article MORPHOLOGICAL FEATURES OF BIODEGRADATION AND BIOCOMPATIBILITY OF COMBINED POLYMER MATRICES BASED ON CHITOSAN AND HYALURONIC ACID
- Authors: Zhuravskii S.G.1, Yukina G.Y.2, Sukhorukova E.G.2, Naumenko M.Y.3, Morozkina S.N.4,5, Snetkov P.P.4,5
-
Affiliations:
- Pavlov First St. Petersburg State Medical University
- Academician I.P. Pavlov First Saint Petersburg State Medical University
- Academician I.P. Pavlov First St. Petersburg State Medical University
- ITMO University
- Kabardino-Balkarian State University named after H.M. Berbekov.
- Section: Original research
- Published: 15.12.2024
- URL: https://journals.eco-vector.com/MAJ/article/view/642129
- DOI: https://doi.org/10.17816/MAJ642129
- ID: 642129
Cite item
Full Text
Abstract
Background. In the development of bioengineered materials used in reconstructive surgery, tissue response to the implanted product is of key importance. Modern possibilities to change synthesis conditions allow to obtain materials with different biodegradation and biocompatibility properties from one polymer.
Objective. To evaluate the character of biodegradation of combined chitosan-containing films
obtained with the addition of hyaluronic acid and under the temperature regime of synthesis.
Materials and Methods. Subcutaneous implantation of four samples two-component
polysaccharide films obtained by irrigating solutions of chitosan with molecular mass 500 and 900 kDa and hyaluronic acid with MM 1300 kDa with temperature treatment (100°C for 5 min) was performed on 30 Wistar rats (weight 200-220 g). The pattern of biodegradation and biocompatibility were evaluated at 60 days by histologic analysis.
Results. The postimplantation period showed the absence of acute toxicity, septic and allergic
inflammation, as well as gross deforming scarring of the surrounding tissues. It was noted that biodegradation of polyelectrolyte complex of chitosan with hyaluronic acid passes through the stage of simultaneous swelling and self-decomposition of the matrix. The result of implantation is reactive inflammation of aseptic character, without the presence of giant multinucleated cells of foreign bodies and intensive growth of connective tissue: replacement of the whole thickness of the degrading matrix and formation of peri-implantation capsule.
Conclusion.
Changing the technological mode is a way to obtain polymer films with a new character of biodegradation, adapted to the clinical tasks of implantation. Molecular mass and selection of technological conditions for obtaining films based on polyelectrolyte complex of chitosan with hyaluronic acid become factors that regulate the rate of biodegradation of polymer matrix in the recipient tissue, which allows solving specific clinical problems on reconstruction of tissue and organ defects replacement zones.
Full Text
About the authors
Sergei G. Zhuravskii
Pavlov First St. Petersburg State Medical University
Author for correspondence.
Email: s.jour@mail.ru
SPIN-code: 5294-2096
доктор медицинских наук, ведущий научный сотрудник лаборатории слуха и речи НИЦ
ORCID: 0000-0002-5960-068Х
Russian FederationGalina Yu. Yukina
Academician I.P. Pavlov First Saint Petersburg State Medical University
Email: pipson@inbox.ru
ORCID iD: 0000-0001-8888-4135
SPIN-code: 2533-2084
Assistant Professor
Russian Federation, Saint PetersburgElena G. Sukhorukova
Academician I.P. Pavlov First Saint Petersburg State Medical University
Email: len48@inbox.ru
ORCID iD: 0000-0001-5521-7248
SPIN-code: 2115-9041
Russian Federation, Saint Petersburg
Maria Yu. Naumenko
Academician I.P. Pavlov First St. Petersburg State Medical University
Email: naumenkomyu@gmail.com
ORCID iD: 0009-0003-8053-6381
Russian Federation, Saint Petersburg
Svetlana N. Morozkina
ITMO University; Kabardino-Balkarian State University named after H.M. Berbekov.
Email: i_norik@mail.ru
ORCID iD: 0000-0003-0122-0251
SPIN-code: 3215-0328
PhD, Associate Professor, Engineer
Russian Federation, Kronverkskiy pr. 49, bldg. A, St. Petersburg, 197101 Russia; St. Chernyshevsky, 173, 360004 Nalchik, Russia.Petr P. Snetkov
ITMO University; Kabardino-Balkarian State University named after H.M. Berbekov.
Email: ppsnetkov@itmo.ru
ORCID iD: 0000-0001-9949-5709
SPIN-code: 2951-3791
Scopus Author ID: 57205168040
Cand. Sci. (Technology)
Russian Federation, Kronverkskiy pr. 49, bldg. A, St. Petersburg, 197101 Russia; St. Chernyshevsky, 173, 360004 Nalchik, Russia.References
- Valachová K., Šoltés L. Versatile use of chitosan and hyaluronan in medicine. Molecules. 2021; 26 (4): 1195. https://doi.org/10.3390/molecules26041195.
- Correia C.R., Moreira-Teixeira L.S., Moroni L., Reis R.L., van Blitterswijk C.A., Karperien M., Mano J.F. Chitosan scaffolds containing hyaluronic acid for cartilage tissue engineering. Tissue engineering. Part C, Methods. 2011; 17 (7): 717–730. https://doi.org/10.1089/ten.tec.2010.0467.
- Collins M.N., Birkinshaw C. Hyaluronic acid based scaffolds for tissue engineering--a review. Carbohydrate polymers. 2013; 92(2): 1262–1279. https://doi.org/10.1016/j.carbpol.2012.10.028.
- Muzzarelli R.A., Greco F., Busilacchi A., Sollazzo V., Gigante A. Chitosan, hyaluronan and chondroitin sulfate in tissue engineering for cartilage regeneration: a review. Carbohydrate polymers. 2012; 89(3): 723–739. https://doi.org/10.1016/j.carbpol.2012.04.057.
- Yasin A., Ren Y., Li J., Sheng Y., Cao C., Zhang K. Advances in hyaluronic acid for biomedical applications. Front Bioeng Biotechnol. 2022; 10: 910290. https://doi.org/10.3389/fbioe.2022.910290.
- Di Mola A., Landi M.R., Massa A., D’Amora U., Guarino V. Hyaluronic acid in biomedical fields: new trends from chemistry to biomaterial applications. Int. J. Mol. Sci. 2022; 23 (22): 14372. https://doi.org/10.3390/ijms232214372.
- De Sousa V.R., da Cunha Santos A.M., de Sousa, B.V., de Araújo Neves G., de Lima Santana L.N., Menezes R.R. A review on chitosan’s uses as biomaterial: tissue engineering, drug delivery systems and cancer treatment. Materials. 2020; 13 (21): 4995. https://doi.org/10.3390/ma13214995.
- Shi C., Zhu Y., Ran X., Wang M., Su Y., Cheng T. Therapeutic potential of chitosan and its derivatives in regenerative medicine. J. Surg. Res. 2006; 133 (2): 185-192. https://doi.org/10.1016/j.jss.2005.12.013.
- Pratiwi A.R., Yuliati A., Soepribadi I., Ariani M.D. Application of chitosan scaffolds on vascular endothelial growth factor and fibroblast growth factor 2 expressions in tissue engineering principles. Dental Journal: Majalah Kedokteran Gigi. 2015; 48 (4): 213-216. https://doi.org/10.20473/j.djmkg.v48.i4.p213-216.
- Feng P., Luo Y., Ke C., Qiu H., Wang W., Zhu Y., Hou R., Xu L., Wu S. Chitosan-based functional materials for skin wound repair: mechanisms and applications. Front Bioeng Biotechnol. 2021; 9: 650598. https://doi.org/10.3389/fbioe.2021.650598.
- Fakhari A., Berkland C. Applications and emerging trends of hyaluronic acid in tissue engineering, as a dermal filler and in osteoarthritis treatment. Acta Biomater. 2013; 9 (7):7081-7092. https://doi.org/10.1016/j.actbio.2013.03.005.
- Treushnikov V.M., Viktorova E.A. Principles of manufacturing biocompatible and biostable polymer implants (review). Sovremennye tekhnologii v meditsine – Modern Technologies in Medicine. 2015; 7 (3): 149-171 (in Russ.). https://doi.org/10.17691 /stm2015.7.3.20.
- Voron'ko N.G., Derkach S.R., Sokolan N.I. Interaction of gelatin with chitosan: the influence of polysaccharide concentration. Vestnik MGTU – Herald of the Bauman Moscow State Technical University. 2015; 18 (1): 80-89 (in Russ.).
- Gribinichenko T.N., Uspenskaya M.V., Snetkov P.P., Olekhnovich R.O. Bi-Layered films based on sodium hyaluronate and chitosan as materials for ENT surgery. Proceedings of the 2022 IEEE-EMBS Conference on Biomedical Engineering and Sciences (IECBES); 2022 Dec 7-9; Kuala Lumpur, Malaysia; 338–343 pp.
- Iaconisi G.N., Lunetti P., Gallo N., Cappello A.R., Fiermonte G., Dolce V., Capobianco L. Hyaluronic acid: a powerful biomolecule with wide-ranging applications – a comprehensive review. Int. J. Mol. Sci. 2023; 24 (12): 10296. https://doi.org/10.3390/ijms241210296].
- Thakhiew W., Devahastin S., Soponronnarit S. Physical and mechanical properties of chitosan films as affected by drying methods and addition of antimicrobial agent. J. Food Eng. 2013; 119 (1): 140-149. https://doi.org/10.1016/j.jfoodeng.2013.05.020.
- Xu J., Liu K., Chang W., Chiou B.-S., Chen M., Liu F. Regulating the physicochemical properties of chitosan films through concentration and neutralization. Foods. 2022; 11 (11): 1657. https://doi.org/10.3390/foods11111657.
- Popryadukhin P.V., Yukina G.Yu., Dobrovol'skaya I.P., Ivan'kova E.M., Yudin V.E. Cell bases of bioresorption of porous 3D-matrix based on chitosan. Tsitologiya - Tsitologiya. 2019; 61 (7): 556-563 (in Russ.).
Supplementary files
СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).