MORPHOLOGICAL FEATURES OF BIODEGRADATION AND BIOCOMPATIBILITY OF COMBINED POLYMER MATRICES BASED ON CHITOSAN AND HYALURONIC ACID



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Abstract

Background. In the development of bioengineered materials used in reconstructive surgery, tissue response to the implanted product is of key importance. Modern possibilities to change synthesis conditions allow to obtain materials with different biodegradation and biocompatibility properties from one polymer.

Objective. To evaluate the character of biodegradation of combined chitosan-containing films

obtained with the addition of hyaluronic acid and under the temperature regime of synthesis.

Materials and Methods. Subcutaneous implantation of four samples two-component

polysaccharide films obtained by irrigating solutions of chitosan with molecular mass 500 and 900 kDa and hyaluronic acid with  MM 1300 kDa with temperature treatment (100°C for 5 min) was performed on 30 Wistar rats (weight 200-220 g). The pattern of biodegradation and biocompatibility were evaluated at 60 days by histologic analysis.

Results. The postimplantation period showed the absence of acute toxicity, septic and allergic

inflammation, as well as gross deforming scarring of the surrounding tissues. It was noted that biodegradation of polyelectrolyte complex of chitosan with hyaluronic acid passes through the stage of simultaneous swelling and self-decomposition of the matrix. The result of implantation is reactive inflammation of aseptic character, without the presence of giant multinucleated cells of foreign bodies and intensive growth of connective tissue: replacement of the whole thickness of the degrading matrix and formation of peri-implantation capsule.

Conclusion.

Changing the technological mode is a way to obtain polymer films with a new character of biodegradation, adapted to the clinical tasks of implantation. Molecular mass and selection of technological conditions for obtaining films based on polyelectrolyte complex of chitosan with hyaluronic acid become factors that regulate the rate of biodegradation of polymer matrix in the recipient tissue, which allows solving specific clinical problems on reconstruction of tissue and organ defects replacement zones.

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About the authors

Sergei G. Zhuravskii

Pavlov First St. Petersburg State Medical University

Author for correspondence.
Email: s.jour@mail.ru
SPIN-code: 5294-2096

доктор медицинских наук, ведущий научный сотрудник лаборатории слуха и речи НИЦ 

ORCID: 0000-0002-5960-068Х

Russian Federation

Galina Yu. Yukina

Academician I.P. Pavlov First Saint Petersburg State Medical University

Email: pipson@inbox.ru
ORCID iD: 0000-0001-8888-4135
SPIN-code: 2533-2084

Assistant Professor

Russian Federation, Saint Petersburg

Elena G. Sukhorukova

Academician I.P. Pavlov First Saint Petersburg State Medical University

Email: len48@inbox.ru
ORCID iD: 0000-0001-5521-7248
SPIN-code: 2115-9041
Russian Federation, Saint Petersburg

Maria Yu. Naumenko

Academician I.P. Pavlov First St. Petersburg State Medical University

Email: naumenkomyu@gmail.com
ORCID iD: 0009-0003-8053-6381
Russian Federation, Saint Petersburg

Svetlana N. Morozkina

ITMO University; Kabardino-Balkarian State University named after H.M. Berbekov.

Email: i_norik@mail.ru
ORCID iD: 0000-0003-0122-0251
SPIN-code: 3215-0328

PhD, Associate Professor, Engineer

Russian Federation, Kronverkskiy pr. 49, bldg. A, St. Petersburg, 197101 Russia; St. Chernyshevsky, 173, 360004 Nalchik, Russia.

Petr P. Snetkov

ITMO University; Kabardino-Balkarian State University named after H.M. Berbekov.

Email: ppsnetkov@itmo.ru
ORCID iD: 0000-0001-9949-5709
SPIN-code: 2951-3791
Scopus Author ID: 57205168040

Cand. Sci. (Technology)

Russian Federation, Kronverkskiy pr. 49, bldg. A, St. Petersburg, 197101 Russia; St. Chernyshevsky, 173, 360004 Nalchik, Russia.

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