SYSTEMIC EFFECTS OF CHRONIC INHALATION OF AN AIR MIXTURE WITH SINGLET OXYGEN IN RATS



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Abstract

Background. Inhalation of technologically generated singlet oxygen via the respiratory system is empirically applied in fitness, sports medicine and proposed to be used in the clinic. At the same time, the mechanisms of action and safety of this mode of action are not fully evident.

Objective. The aim of the study was to evaluate in vivo systemic effects of prolonged inhalation of air mixture enriched with singlet oxygen.

Materials and Methods. The study was conducted on 8 rats (200-220 g, female Wistar stock) kept in a barrier-type vivarium. The experimental group (singlet oxygen, n=4) received singlet oxygen-enriched air mixture for free breathing 3 times a day for 40 minutes daily for 4 months in an individually ventilated cage via SmartFlow ventilation system. Singlet oxygen was generated by the original photocatalytic method using a specially designed device. Intact rats (n=4) were used as controls. The weight of animals, hematologic parameters of blood, total antioxidant and prooxidant activities in plasma, level of malonic dialdehyde in tissues and histologic picture of organs were evaluated.

Results. No clinical signs of general toxicity (hair loss, inflammatory changes of visible mucous membranes, diarrhea), demonstration of behavioral signs of stress were observed in the singlet oxygen group. There were no differences in weight between the groups. Among hematologic parameters, an increase in hemoglobin concentration was noted (p=0.028). No differences in total antioxidant and pro-oxidant activities of plasma were found. At the same time, the intensity of peroxidative processes by malonic dialdehyde level in kidney tissues was significantly lower than the background values of the group of intact animals (p=0,029). The histologic picture of the ciliary apparatus of the nasal septum mesenteric epithelium, lung parenchyma, liver, kidney, heart, spleen, thymus did not differ from the norm.

Conclusion. In vivo safety of prolonged inhalation of air mixture with singlet oxygen has been demonstrated. The dynamics of lipid peroxidation indices with preserved histological picture allows us to speak about adaptation reactions in tissues and organs arising in response to breathing air enriched with singlet oxygen. We believe that the noted systemic changes occur according to the pathophysiological mechanism of remote preconditioning.

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About the authors

Sergei G. Zhuravskii

Almazov National Medical Research Centre
ITMO University

Author for correspondence.
Email: s.jour@mail.ru
SPIN-code: 5294-2096
Scopus Author ID: 8244733500

д.м.н., вед. н. с. – руководитель НИГр патологии сенсорных систем НЦМУ ФГБУ «НМИЦ им. В. А. Алмазова» Минздрава Российской Федерации; пр. Пархоменко, дом 15, лит. Б. Санкт-Петербург, Российская Федерация;

инженер, университет ИТМО. 197101, Кронверкский пр., д. 49, лит. А. Санкт-Петербург, Россия.

ORCID: 0000-0002-5960-068Х;

Russian Federation, 2 Akkuratova str., Saint- Petersburg, 197341; Kronverkskiy av., 49 A, St. Petersburg, 197101 Russia;

Igor K. Meshkovskii

ITMO University

Email: meshkovskyik@gmail.com
ORCID iD: 0000-0003-3470-1000
SPIN-code: 3122-7610
Scopus Author ID: 575174

профессор, д.т.н, директор, главный научный сотрудник научно-исследовательского центра световодной фотоники, профессор Института «Высшая инженерная школа» Университета ИТМО. 197101, Кронверкский пр., д. 49, лит. А. Санкт-Петербург, Россия.

Russian Federation, Kronverkskiy av., 49 A, St. Petersburg, 197101 Russia

Ekaterina Yu. Podyacheva

Almazov National Medical Research Centre

Email: ekaterinapodyachevaspb@gmail.com
ORCID iD: 0000-0002-0365-3301
SPIN-code: 5613-6297
Scopus Author ID: 1040642

младший научный сотрудник НИЛ биопротезирования и кардиопротекции ИЭМ ФГБУ «НМИЦ им. В. А. Алмазова» Минздрава России, пр. Пархоменко, дом 15, лит. Б. Санкт-Петербург, Российская Федерация;

Russian Federation, 2 Akkuratova str., Saint- Petersburg, 197341

Galina A. Shulmeyster

Almazov National Medical Research Centre.

Email: g.schulmeister@yandex.ru
ORCID iD: 0000-0002-8949-1605
SPIN-code: 1606-2565
Scopus Author ID: 1071616

младший научный сотрудник научно-исследовательской лаборатории нанотехнологий ЦЭБ ИЭМ ФГБУ «НМИЦ им. В. А. Алмазова» Минздрава Российской Федерации, Санкт-Петербург, пр. Пархоменко, дом 15, лит. Б.

Russian Federation, 2 Akkuratova str., Saint- Petersburg, 197341.

irina А. Zelinskaya

Almazov National Medical Research Centre.

Email: irina.selinskaja@gmail.com
ORCID iD: 0000-0002-1971-3444
SPIN-code: 1203-7167
Scopus Author ID: 890600

научный сотрудник НИЛ Биопротезирования и кардиопротекции ЦЭБ ИЭМ ФГБУ «НМИЦ им. В. А. Алмазова». Минздрава Российской Федерации;

Russian Federation, 2 Akkuratova str., Saint- Petersburg, 197341

Semen А. Plyastsov

ITMO University.

Email: s.plyastsov@gmail.com
ORCID iD: 0000-0002-5764-0960
SPIN-code: 3596-6642
Scopus Author ID: 761378

к.т.н., заведующий лабораторией научно-исследовательского центра световодной фотоники, начальник лаборатории технологических методов и устройств Университета ИТМО. 197101, Кронверкский пр., д. 49, лит. А. Санкт-Петербург, Россия.

Russian Federation, Kronverkskiy av., 49 A, St. Petersburg, 197101 Russia;

Larisa L. Khomutinnikova

ITMO University

Email: larahlesnyh@yandex.ru
ORCID iD: 0000-0003-0918-6350
SPIN-code: 8780-8255
Scopus Author ID: 1273282

инженер, университет ИТМО;

Russian Federation, Kronverkskiy av., 49 A, St. Petersburg, 197101 Russia;

Sergei K. Evstropiev

ITMO University

Email: evstropiev@bk.ru
ORCID iD: 0000-0002-0160-8443
SPIN-code: 7597-9308
Scopus Author ID: 6507317768

профессор, д.х.н., Санкт-Петербургский государственный технологический институт (Технический университет)

Russian Federation, Kronverkskiy av., 49 A, St. Petersburg, 197101 Russia;

Alexey G. Baindurashvili

H. Turner National Medical Research Center for Children's Orthopedics and Trauma Surgery,

Email: turner011@mail.ru
ORCID iD: 0000-0001-8123-6944
SPIN-code: 2153-9050
Scopus Author ID: 6603212551

академик АН РФ, доктор медицинских наук, профессор, директор Института детской хирургии, травматологии и ортопедии Санкт-Петербургского Государственного педиатрического медицинского университета.

Russian Federation, Parkovaya str. 64, Pushkin, Saint-Petersburg, 196603.

Vladimir N. Vasiliev

ITMO University

Email: vnvasilev@itmo.ru
SPIN-code: 2427-9016
Scopus Author ID: 8388135700
ResearcherId: B-6386-2015

 член.-кор. АН РФ, ректор, доктор технических наук, профессор.

ORCID: 0000-0001-5572-926X;

Russian Federation, Kronverkskiy av., 49 A, St. Petersburg, 197101 Russia;

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