Email this article Procoagulant platelets in thrombi from arteries and veins of the lower extremities.
- Authors: Obydennyi S.I.1,2, Kireev I.I.3, Panteleev M.A.1,2,3
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Affiliations:
- The Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology of the Ministry of Healthcare of the Russian Federation, Moscow
- Center for Theoretical Problems of Physical and Chemical Pharmacology of the Russian Academy of Sciences, Moscow
- Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia
- Section: Original research
- Published: 29.05.2025
- URL: https://journals.eco-vector.com/MAJ/article/view/677736
- DOI: https://doi.org/10.17816/MAJ677736
- ID: 677736
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Abstract
In this work, we investigate the presence of procoagulant platelets in thrombi extracted from arteries and veins of the lower extremities of patients with circulatory disorders.
The aim of the study was to select a technique suitable for determining the presence of procoagulant platelets in thrombi of veins and arteries of the lower extremities.
The ultrastructure of the extracted thrombi was studied using transmission electron microscopy.
The results showed that procoagulant platelets were absent in two arterial thrombi and were found only in one venous thrombus. This absence may be due to the destruction of platelets and changes in their morphology. It is important to note that platelet aggregates constituted only a small part of the venous thrombus, which does not exclude the possible contribution of procoagulant platelets at the early stages of thrombus formation. In addition, cellular debris with potential procoagulant activity were observed in the samples.
Conclusions: Transmission electron microscopy allows detection of procoagulant platelets in thrombi of arteries and veins of lower extremities. Further sampling is required to draw conclusions about the prevalence of the procoagulant subpopulation in the studied thrombi. In addition, when studying subpopulations in venous and arterial thrombi, platelet morphology visualization is necessary, since a large number of destructed cell fragments with potential procoagulant activity were found in them.
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About the authors
Sergei Ivanovich Obydennyi
The Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology of the Ministry of Healthcare of the Russian Federation, Moscow;Center for Theoretical Problems of Physical and Chemical Pharmacology of the Russian Academy of Sciences, Moscow
Author for correspondence.
Email: obydennyi@yandex.ru
ORCID iD: 0000-0002-2930-8768
SPIN-code: 4193-0858
Igor Igorevich Kireev
Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia
Email: iikireev@gmail.com
ORCID iD: 0000-0001-9252-6808
SPIN-code: 1724-6395
Mikhail Alexandrovich Panteleev
The Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology of the Ministry of Healthcare of the Russian Federation, Moscow;Center for Theoretical Problems of Physical and Chemical Pharmacology of the Russian Academy of Sciences, Moscow;
Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia
Email: mapanteleev@yandex.ru
ORCID iD: 0000-0002-8128-7757
SPIN-code: 4701-6344
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СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).