Procoagulant platelets in thrombi from arteries and veins of the lower extremities.



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Abstract

In this work, we investigate the presence of procoagulant platelets in thrombi extracted from arteries and veins of the lower extremities of patients with circulatory disorders.

The aim of the study was to select a technique suitable for determining the presence of procoagulant platelets in thrombi of veins and arteries of the lower extremities.

The ultrastructure of the extracted thrombi was studied using transmission electron microscopy.

The results showed that procoagulant platelets were absent in two arterial thrombi and were found only in one venous thrombus. This absence may be due to the destruction of platelets and changes in their morphology. It is important to note that platelet aggregates constituted only a small part of the venous thrombus, which does not exclude the possible contribution of procoagulant platelets at the early stages of thrombus formation. In addition, cellular debris with potential procoagulant activity were observed in the samples.

Conclusions: Transmission electron microscopy allows detection of procoagulant platelets in thrombi of arteries and veins of lower extremities. Further sampling is required to draw conclusions about the prevalence of the procoagulant subpopulation in the studied thrombi. In addition, when studying subpopulations in venous and arterial thrombi, platelet morphology visualization is necessary, since a large number of destructed cell fragments with potential procoagulant activity were found in them.

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About the authors

Sergei Ivanovich Obydennyi

The Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology of the Ministry of Healthcare of the Russian Federation, Moscow;
Center for Theoretical Problems of Physical and Chemical Pharmacology of the Russian Academy of Sciences, Moscow

Author for correspondence.
Email: obydennyi@yandex.ru
ORCID iD: 0000-0002-2930-8768
SPIN-code: 4193-0858

Igor Igorevich Kireev

Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia

Email: iikireev@gmail.com
ORCID iD: 0000-0001-9252-6808
SPIN-code: 1724-6395

Mikhail Alexandrovich Panteleev

The Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology of the Ministry of Healthcare of the Russian Federation, Moscow;
Center for Theoretical Problems of Physical and Chemical Pharmacology of the Russian Academy of Sciences, Moscow;
Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia

Email: mapanteleev@yandex.ru
ORCID iD: 0000-0002-8128-7757
SPIN-code: 4701-6344

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