Myeloperoxidase/high-density lipoprotein cholesterol ratio in patients with arterial hypertension and chronic coronary heart disease
- Authors: Churashova I.A.1, Sokolov A.V.1,2, Kostevich V.A.1, Gorbunov N.P.1, Runova O.L.1, Firova E.M.1, Vasilyev V.B.1,2
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Affiliations:
- Institute of Experimental Medicine
- Saint Petersburg State University
- Issue: Vol 21, No 2 (2021)
- Pages: 75-86
- Section: Original research
- Published: 24.09.2021
- URL: https://journals.eco-vector.com/MAJ/article/view/71486
- DOI: https://doi.org/10.17816/MAJ71486
- ID: 71486
Cite item
Abstract
BACKGROUND: Myeloperoxidase (MPO), the enzyme of leukocytes, catalyzes the production of reactive halogen species, which can modify the structure of lipoproteins. Chlorination and nitration of tyrosine residues in apolipoprotein A-1 lead to the formation of dysfunctional high-density lipoproteins (HDL-p), thus blocking the reverse cholesterol transport. Low level of high-density lipoprotein cholesterol (HDL-C) is associated with exacerbation of coronary heart disease, but the prognostic value of this index is not fully assessed.
AIM: The aim of this study was to examine a possible contribution of MPO to the atherosclerotic plaque development (the stable growth or the erosion and rupture) via the modification of HDL-p. That is to say we investigated the diagnostic values of measuring the total MPO (MPO-T), the active MPO (MPO-A) and the MPO/HDL-С relation in patients with hypertension and various forms of chronic coronary heart disease.
MATERIALS AND METHODS: The cohort under study included 44 patients with arterial hypertension and chronic coronary heart disease. All patients were divided into three groups according to the diagnosis: arterial hypertension without coronary heart disease (Group I, n = 20); arterial hypertension and the initially stable chronic coronary heart disease without acute complications in the anamnesis (Group II, n = 14); arterial hypertension and myocardial infarction (acute coronary syndrome) in the anamnesis (Group III, n = 10). The enzyme-linked immunosorbent assay (ELISA) for MPO-T and specific immuno-extraction followed by enzymatic detection (SIEFED) by fluorogenic substrate for MPO-A were applied. After that the ratio MPO-T/HDL-C or MPO-A/HDL-C was calculated.
RESULTS: The MPO-A and MPO-A/HDL-C ratio were significantly increased in the group III of patients with old myocardial infarction as compared with the patients of group II who had the initially stable coronary heart disease (p = 0.009 and p = 0.003, respectively). Besides, the level of HDL-C in the group III was significantly reduced (p = 0.013). Our measurements revealed the negative correlation between MPO-A and HDL-C concentrations (r = –0.31; p < 0.05), which is in line with the presumption of the study accomplished. Surprisingly, the correlation between MPO-T/HDL-C ratio and that MPO-A/HDL-C was stronger (r = 0.72; p < 0.05), than between MPO-T and MPO-A (r = 0.36; p < 0.05).
CONCLUSIONS: Our study demonstrates the importance of assessing MPO-T and MPO-A plasma concentrations and of calculating the ratio MPO/HDL-C as promising biomarkers in the complicated cases of chronic coronary heart disease. MPO-A and MPO-A/HDL-C values were elevated in the patients with old myocardial infarction, while the concentration of HDL-C remained decreased upon the transition from the acute to chronic phase of the disease.
Full Text
About the authors
Irina A. Churashova
Institute of Experimental Medicine
Email: churashova@iemspb.ru
ORCID iD: 0000-0001-8064-6861
SPIN-code: 5916-3140
Senior Researcher of the Department of Molecular Genetics
Russian Federation, Saint-PetersburgAlexey V. Sokolov
Institute of Experimental Medicine; Saint Petersburg State University
Author for correspondence.
Email: biochemsokolov@gmail.com
ORCID iD: 0000-0001-9033-0537
SPIN-code: 7427-7395
Doctor of Biological Sciences, Head of the Laboratory of Biochemical Genetics of the Department of Molecular Genetics, Professor of Chair of Fundamental Problems of Medicine and Medical Technology
Russian Federation, Saint-PetersburgValeria A. Kostevich
Institute of Experimental Medicine
Email: hfa-2005@yandex.ru
ORCID iD: 0000-0002-1405-1322
SPIN-code: 2726-2921
PhD (Biology), Senior Researcher of the Department of Molecular Genetics
Russian Federation, Saint-PetersburgNikolay P. Gorbunov
Institute of Experimental Medicine
Email: niko_laygo@mail.ru
ORCID iD: 0000-0003-4636-0565
SPIN-code: 6289-7281
Research fellow of the Department of Molecular Genetics
Russian Federation, Saint-PetersburgOlga L. Runova
Institute of Experimental Medicine
Email: o.runowa@yandex.ru
Candidate of Biological Sciences, Junior Researcher of the Department of Molecular Genetics
Russian Federation, Saint-PetersburgElvira M. Firova
Institute of Experimental Medicine
Email: Firova@yandex.ru
Candidate of Medical Sciences, Head of the Department of Cardiology
Russian Federation, Saint-PetersburgVadim B. Vasilyev
Institute of Experimental Medicine; Saint Petersburg State University
Email: vadim@biokemis.ru
ORCID iD: 0000-0002-9707-262X
SPIN-code: 6699-6350
Doctor of Medical Sciences, Head of the Department of Molecular Genetics, Professor of Chair of Fundamental Problems of Medicine and Medical Technology
Russian Federation, Saint-PetersburgReferences
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