Polymorphism of TLR genes and the course of COVID-19 bilateral pneumonia
- Authors: Evdokimov A.V.1, Suslova T.A.1,2, Belyaeva S.V.1,2, Burmistrova A.L.1, Stashkevich D.S.1
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Affiliations:
- Chelyabinsk State University
- Chelyabinsk Regional Hemotransfusion Station
- Issue: Vol 21, No 4 (2021)
- Pages: 57-66
- Section: Original research
- URL: https://journals.eco-vector.com/MAJ/article/view/90324
- DOI: https://doi.org/10.17816/MAJ90324
Cite item
Abstract
BACKGROUND: COVID-19 is a disease which course depends on a number of factors, including genetic ones, among which the genes of the innate immune system receptors – TLR (toll-like receptors), which play a central role in the development of innate immunity reactions, are of particular interest. The SARS-CoV-2 virus structure includes, in addition to the nucleocapsid, a protein-lipid membrane envelope, which determines the recognition of virus components by different TLRs, including TLR2 subfamily receptors (TLR1, 6, 10), which genetic polymorphisms occur with different frequencies in different human populations and affect not only the functional activity of the innate immunity but also determine the quality of the adaptive immune response.
AIM: The study aimed to determine the association of polymorphisms of toll-like receptor genes TLR1, TLR6 and TLR10 with the severity of coronavirus infection (COVID-19) in the Russian population of the Chelyabinsk region.
MATERIALS AND METHODS: The study included 86 patients from COVID-departments of hospitals in Chelyabinsk with a diagnosis of bilateral pneumonia with a degree of severity: moderate (M-BLP, n = 36) or severe (S-BLP, n = 50). The control group consisted of 100 healthy individuals from the register of the Chelyabinsk regional hemotransfusion station (“Control”). All the individuals studied belonged to the Russian ethnic group. Polymorphisms 1805T>G of TLR1 gene, 745C>T of TLR6 gene and 721A>C of TLR10 gene were determined using polymerase chain reaction with restriction fragment length polymorphism. The analysis of the association between genotypes and the status of individuals was carried out using the correspondence analysis and the Monte Carlo method.
RESULTS: It was revealed that the differences between the studied groups are completely determined by TLR1 genotypes. The GG genotype with statistical significance is more often detected in the “Control” group compared to M-BLP and S-BLP (p < 0.001, OR = 12.94) and can be assessed as protective in relation to the development of bilateral pneumonia in COVID-19. The TT genotype can be considered as predisposing to the development of a severe form of bilateral pneumonia in COVID-19 (p = 0.022): the TT genotype is significantly less common (OR = 0.20) in the M-BLP group compared to S-BLP.
CONCLUSIONS: It can be assumed that the genetic variant 1805*G of the TLR1 gene, which provides a moderate pro-inflammatory response and predominates in European populations, gives an advantage to its owners, preventing the development of complicated conditions in COVID-19 infection.
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About the authors
Alexander V. Evdokimov
Chelyabinsk State University
Author for correspondence.
Email: avdax@yandex.ru
ORCID iD: 0000-0002-7011-368X
SPIN-code: 9092-4429
Scopus Author ID: 56946405800
ResearcherId: ABA-8628-2021
Cand. Sci. (Biol.), Assistant Professor at the Department of Microbiology, Immunology and General Biology of the Biology Faculty
Russian Federation, 129, Bratiev Kashirinykh Str., Chelyabinsk, 454001Tatyana A. Suslova
Chelyabinsk State University; Chelyabinsk Regional Hemotransfusion Station
Email: hla_chel@mail.ru
ORCID iD: 0000-0002-7028-6839
SPIN-code: 2869-1066
MD, Cand. Sci. (Med.), Assistant Professor, Head of the Laboratory of Immunological Research, Assistant Professor at the Department of Microbiology, Immunology and General Biology of the Biology Faculty
Russian Federation, 129, Bratiev Kashirinykh Str., Chelyabinsk, 454001; ChelyabinskSvetlana V. Belyaeva
Chelyabinsk State University; Chelyabinsk Regional Hemotransfusion Station
Email: shshvetlana@gmail.com
SPIN-code: 9485-3361
Cand. Sci. (Biol.), biologist of the Laboratory of Immunological Research, Assistant Professor at the Department of Microbiology, Immunology and General Biology of the Biology Faculty
Russian Federation, 129, Bratiev Kashirinykh Str., Chelyabinsk, 454001; ChelyabinskAlexandra L. Burmistrova
Chelyabinsk State University
Email: burmal@csu.ru
ORCID iD: 0000-0001-6462-9500
SPIN-code: 2374-7309
MD, Dr. Sci. (Med.), Professor, Head of the Department of Microbiology, Immunology and General Biology of the Biology Faculty
Russian Federation, 129, Bratiev Kashirinykh Str., Chelyabinsk, 454001Darya S. Stashkevich
Chelyabinsk State University
Email: stashkevich_dary@mail.ru
SPIN-code: 6592-1469
Cand. Sci. (Biol.), Assistant Professor, Dean of the Biology Faculty
Russian Federation, 129, Bratiev Kashirinykh Str., Chelyabinsk, 454001References
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