INVESTIGAION OF PRODUCTION OF A NON-TOXIC DIPHTHERIA TOXIN VARIANT CRM197 IN ESCHERICHIA COLI CELLS
- Authors: Dukhovlinov IV1, Bogomolova EG1, Dobrovolskaya OV1, Ishuk C.A1, Fedorova E.A1, Klimov NA1, Simbirtsev AS1
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Affiliations:
- FSUE «State Research Institute for Highly Pure Biopreparations» FMBA of the Russian Federation
- Issue: Vol 18, No 1 (2018)
- Pages: 64-70
- Section: Articles
- Published: 15.03.2018
- URL: https://journals.eco-vector.com/MAJ/article/view/9383
- DOI: https://doi.org/10.17816/MAJ18164-70
- ID: 9383
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Abstract
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About the authors
I V Dukhovlinov
FSUE «State Research Institute for Highly Pure Biopreparations» FMBA of the Russian Federation
E G Bogomolova
FSUE «State Research Institute for Highly Pure Biopreparations» FMBA of the Russian Federation
O V Dobrovolskaya
FSUE «State Research Institute for Highly Pure Biopreparations» FMBA of the Russian Federation
CA A Ishuk
FSUE «State Research Institute for Highly Pure Biopreparations» FMBA of the Russian Federation
EA A Fedorova
FSUE «State Research Institute for Highly Pure Biopreparations» FMBA of the Russian Federation
N A Klimov
FSUE «State Research Institute for Highly Pure Biopreparations» FMBA of the Russian Federation
A S Simbirtsev
FSUE «State Research Institute for Highly Pure Biopreparations» FMBA of the Russian Federationcorresponding member of the Russian Academy of Sciences
References
- Lory S., Carroll S.F., Collier R.J. Ligand interactions of diphtheria toxin. II. Relationships between the NAD site and the P site // J. Biol. Chem. 1980. Vol. 255, No 24. P. 12016-12019.
- Uchida T, Pappenheimer A.M., Greany R. Diphtheria toxin and related proteins. I. Isolation and properties of mutant proteins serologically related to diphtheria toxin // J. Biol. Chem. 1973. Vol. 248, No 11. P. 3838-3844.
- Durando P, Faust S.N., Fletche M, Krizova P, Torres A., Welte T. Experience with pneumococcal polysaccharide conjugate vaccine (conjugated to CRM197 carrier protein) in children and adults // Clin. Microbiol. Infect. 2013. Vol. 19 (suppl. 1). P. 1$9.
- Avci F.Y, Li X., Tsuji M, Kasper D.L. A mechanism for glycoconjugate vaccine activation of the adaptive immune system and its implications for vaccine design // Nat. Med. 2011. Vol. 17, No 12. P. 1602-1609.
- Burtnick M.N., Shaffer T.L., Ross B.N., Muruato L.A., Sbrana E, DeShazer D, Torres A.G., Brett P.J. Development of Subunit Vaccines that Provide High Level Protection and Sterilizing Immunity Against Acute Inhalational Melioidosis // Infect. Immun. 2017. Nov. 6. pii: IAI.00724-17. doi: 10.1128/ IAI.00724-17.
- Ilyina N, Kharit S., Namazova-Baranova L., Asatryan A., Benashvili M., Tkhostova E., Bhusal C., Arora A.K. Safety and immunogenicity of meningococcal ACWYCRM197-conjugate vaccine in children, adolescents and adults in Russia // Hum. Vaccin. Immunother. 2014. Vol. 10, No 8. P. 2471-2481.
- Allison E.B., Turner J.E., Gerson H.Y, So, D.J., Krasznai A.J., Hilaire S, Gerson D.F. Novel polysaccharide-protein conjugates provide an immunogenic 13-valent pneumococcal conjugate vaccine for S. pneumoniae // Synth. Syst. Biotechnol. 2017. Vol. 2, No 1. P. 49$58.
- Dateoka S., Ohnishi Y., Kakudo K. Effects of CRM197, a specific inhibitor of HB-EGF, in oral cancer // Med. Mol. Morphol. 2012. Vol. 45, No 2. P. 91-97.
- Dateoka S., Kakudo K. CRM197, a specific inhibitor of HB-EGF // Med. Mol. Morphol. 2013. Vol. 415, No 1. P. 83-87.
- Schenk G.J., Haasnoot P.C., Centlivre M., Legrand N., Rip J., de Boer A.G., Berkhout B. Efficient CRM197-mediated drug targeting to monocytes // J. Control. Release 2012. Vol. 158, No 1. P. 139-147.
- Rappuoli R. Isolation and characterization of Corynebacterium diphtheria nontandem double lysogens hyperproducing CRM197 // Appl. Environ. Microbiol. 1983. Vol. 46, No 3. P. 560-564.
- Zhou J., Petracca R. Secretory expression of recombinant diphtheria toxin mutants in B. Subtilis // J. Tongji Med. Univ. Tong Ji Yi Ke Xue Xue Bao. 1999. Vol. 19, No 4. P. 253-256.
- Retallack D.M., Jin H., Chew L. Reliable protein production in a Pseudomonas fluorescens expression system // Protein Expr. Purif. 2012. Vol. 81, No 2. P. 157-165.
- Majumder K. Ligation-free gene synthesis by PCR: synthesis and mutagenesis at multiple loci of a chimeric gene encoding OmpA signal peptide and hirudin // Gene. 1992. Vol. 110, No 1. P. 89-94.
- Studier FW. Stable expression clones and auto-induction for protein production in E. coli // Methods Mol Biol. 2014. Vol. 1091, No 1. P. 17-32.
- Laemmly U.K. Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 // Nature. 1970. No 227. P. 680-685.
- Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J. // J. Biol. Chem. 1951. Vol. 193, No 1. P. 265 -275.
- Phadtare S., Severinov K. RNA remodeling and gene regulation by cold shock proteins // RNA Biol. 2010. Vol. 7, No 6. P. 788-795.
- Takara Cold Shock Expression System pCold™ DNA. Product Manual. http://www.takara-bio.com.
- Chang M.P., Baldwin R.L., Bruce C., Wisnieski B.J. Second cytotoxic pathway of diphtheria toxin suggested by nuclease activity // Science. 1989. Vol. 246, No 4934. P. 1165-1168.
- Bruce C., Baldwin R.L., Lessnick S.L., Wisnieski B.J. Diphtheria toxin and its ADP-ribosyltransfer-ase-defective homologue CRM197 possess deoxyribonuclease activity // Proc. Nati. Acad. Sci. USA. 1990. Vol. 87, No 8. P. 2995-2998.
- Jia B., Co J. High-throughput recombinant protein expression in Escherichia coli: current status and future perspectives // Open Biol.2016. Vol. 6, No 8. pii: 160196. doi: 10.1098/rsob.160196.
- Srnrensen H.P, Mortensen K.K. Advanced genetic strategies for recombinant protein expression in Escherichia coli // J. Biotechnol. 2005. Vol. 115, No 2. Р. 113-128.
- Peleg Y, Unger T. Application of high-throughput methodologies to the expression of recombinant proteins in E. coli // Methods Mol. Biol. 2008. Vol. 426. P. 197-208.