THE ROLE OF CHLAMIDIA PNEUMONIA AND TNF-α IN DESTABILIZATION OF ATHEROSCLEROTIC PLAQUE BY HUMAN

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Abstract


Research objective: The aim of this work was a comparative study of the contents of the Chlamydia pneumoniae and TNF-α in the unstable and stable atherosclerotic lesions in humans and their role in the destabilization of atherosclerotic plaques. Material and methods: Investigated 50 aortic tissue samples received from 61 ± 7 years men who died from coronary heart disease. Verification of unstable progressive atherosclerotic plaques was conducted using histological staining Oil Red O and hematoxylin Mayer. Chlamydia pneumoniae identified in two ways: by using monoclonal antibodies and by modified Romanowski-Giemsa. Determination of TNF-α was made highly sensitive two-stage biotin-streptavidin method. Analysis of the results was carried out using a light microscope Leica DM 2500 and computer program «Leica Application Suite Version 3.4.0». Results: In unstable atherosclerotic lesions in the same areas and cell types that make up the inflammatory infiltrates, as well as in cells smooth muscle cells of these plaques are detected simultaneously Chlamydia pneumoniae and powerful inflammation cytokine TNF-α. In stable lesions intra- and extracellular expression of this cytokine is not detected. Conclusion: On the formation of unstable plaques a large impact could have the combined effect of Chlamydia pneumoniae and TNF-α, contributing to the development of the immunoinflamatory process in the vascular wall. Potential mechanism for strengthening action on the severity of the disease may be associated with direct or indirect influence of infection, as damage to the vascular wall, and increased production of proinflammatory cytokines cells, which are involved in the destabilization of plaque.

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About the authors

P V Pigarevsky

Institute of Experimental Medicine


S V Maltseva

Institute of Experimental Medicine


V A Snegova

Institute of Experimental Medicine


N G Davydova

Institute of Experimental Medicine


O G Yakovleva

Institute of Experimental Medicine


R A Vorozhbit

Institute of Experimental Medicine


References

  1. Пигаревский П.В., Мальцева С.В., Селиверстова В.Г. Иммунокомпетентная система, атеросклероз и персистирующая инфекция // Вестник РАМН. 2005. № 2. С. 17-22 [Pigarevsky P.V., Maltseva S.V., Seliverstova V.G. Immunokompetentnaja system, arteriosclerosis and persistant infection, Vestnik RAMN. 2005. Nо 2. pp. 17-22].
  2. Нагорнев В., Анестиади В., Зота Е. Патоморфоз атеросклероза (иммуноаспекты). Санкт-Петербург, Кишинев, 2008. 318 с. [Nagornev V., Anestiady V., Zota E. Pathomorphosis of atherosclerosis (immunoaspects), St. Petersburg, Kishinev, 2008, 318 p.].
  3. Пигаревский П.В., Мальцева С.В., Снегова В.А., Давыдова Н.Г., Гусева В.А. Chlamidia pneumonia и иммуновоспалительные реакции в нестабильной атеросклеротической бляшке у человека // Бюллетень экспериментальной биологии и медицины. 2015. Т. 159, № 2. С. 244-248 [Pigarevsky P.V., Maltseva S. V., Snegova V.A., Davidova N.G., Guseva V.A. Chlamidia pneumonia and immune inflammatory reaction in unstable atherosclerotic plaque in humans, Bul. Experiment. Boil. Fnd Medicine. 2015. vol. 159. Nо 2. pp. 244-248].
  4. Rajtar R., Malczewska-Malec M., Kloch M., Kolasińska-Kloch W. Chlamydia pneumoniae and cardiovascular diseases // Przegl Lek. 2004. Vol. 61 (3). P. 165-169.
  5. Wick G., Knoflach M., Xu Q. Autoimmune and inflammatory mechanisms in atherosclerosis // Ann. Rev. Immunol. 2004. Vol. 22. P. 361-403.
  6. Карагодин В.П., Бобрышев Ю.В., Орехов А.Н. Воспаление, иммунокомпетентные клетки, цитокины - роль в атерогенезе // Патогенез. 2014. Т. 12, № 1. С. 21-35 [Karagodin V.P., Bobrishev Y.V., Orekhov A.N. Inflammation, immune cells, cytokines - role in atherogenesis, Pathogenesis. 2014. vol. 12. Nо 1. pp. 21-35.]
  7. Fragoso L.J.M., Sierra M.M., Vargas A.G., Barrios R.A., Ramírez B.J. Tumor necrosis factor alfa in cardiovascular diseases: molecular biology and genetics // Gac. Med. Mex. 2013. Vol. 149 (5). P. 521-530.
  8. Michowitz Y., Goldstein E., Roth A., Afek A., Abashidze A., Ben Gal Y., Keren G., George J. The involvement of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) in atherosclerosis // J. Am. Coll Cardiol. 2005. Vol. 45 (7). P. 1018-1024.
  9. Пигаревский П.В. Антигены и их роль в иммуновоспалительных реакциях при атерогенезе у человека // Мед. акад. журн. 2010. Т. 10, № 4. С. 210-217 [Pigarevsky P.V. Antigens and their role in immunoinflamation reactions in atherogenesis in humans, Med. Acad. Zhurn. 2010. vol. 10. No. 4. pp. 210-217.]
  10. Blake G.J, Ridker P.M. Novel clinical markers of vascular wall inflammation // Circ. Res. 2001. Vol. 89 (9). P. 763-771.
  11. Erbel C., Sato K., Meyer F.B., Kopecky S.L., Frye R.L., Goronzy J.J., Weyand C.M. Functional profile of activated dendritic cells in unstable atherosclerotic plaque // Basic Res. Cardiol. 2007. Vol. 102 (2). P. 123-132.

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Copyright (c) 2017 Pigarevsky P.V., Maltseva S.V., Snegova V.A., Davydova N.G., Yakovleva O.G., Vorozhbit R.A.

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