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Experimental Autoimmune Encephalomyelitis (EAE) has been used for human autoimmune disease, Multiple Sclerosis (MS), which is characterized by inflammation in the Central Nerve System (CNS). In this review, we focus one of EAE variants, transfer EAE induced by adoptive transfer of encephalitogenic autoantigen specific T-cell clone. We describe about the model and introduce the application of this model for experimental research and therapeutic development. In addition, we introduce two-photon intravital imaging, which enable to observe and track fluorescently labeled encephalitogenic T-cells in living animal in real time. Further, by using activation sensors, which detect cellular activation during intravital imaging, we show migrating T-cells become activate upon contacting with local antigen presenting cells. The obtained results reveal that the transfer EAE model is useful to understand the mechanisms of initiating CNS inflammation.

Naoto Kawakami

Ludwig-Maximilians University; Max-Planck Institute of Neurobiology

Institute of Clinical Neuroimmunology

Hartmut Wekerle

Max-Planck Institute of Neurobiology

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