SOME FEATURES OF THE STRESS REACTION IN RATS AFTER EXPOSURE TO STRESS AND ADMINISTRATION OF ANTIMICROBIAL PEPTIDE DEFENSIN RATNP-3
- Authors: Yankelevich IA1, Aleshina GM1, Kokryakov VN1
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Affiliations:
- Institute of Experimental Medicine of the North-West Branch of the Russian Academy of Medical Sciences
- Issue: Vol 14, No 4 (2014)
- Pages: 63-67
- Section: Articles
- URL: https://journals.eco-vector.com/MAJ/article/view/9815
- DOI: https://doi.org/10.17816/MAJ14463-67
- ID: 9815
Cite item
Abstract
Dynamics of corticosterone concentration and redistribution of leukocytes in the blood of rats under stressful impact and administration of the antimicrobial peptide defensin RatNP-3 have been studied. The experimental model of stress - swimming in cold (2-4° C) water in 2 min. Plasma corticosterone levels were evaluated by IFA kit for corticosterone. The number of leukocytes was counted in Goryaev chamber and a manual WBC differential was performed using blood smears. It have been shown that administration of defensin RatNP-3 immediately before stressful impact have normalized stress-induced changes in the number of neutrophils in the blood, and reduces stress-induced increase of corticosterone concentration in the blood of rats. The results of this study results suggest that defensin can act as an adaptogen during experimental stress in rats.
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About the authors
I A Yankelevich
Institute of Experimental Medicine of the North-West Branch of the Russian Academy of Medical Sciences
Email: irinkab@bk.ru
G M Aleshina
Institute of Experimental Medicine of the North-West Branch of the Russian Academy of Medical Sciences
Email: galina_aleshina@mail.ru
V N Kokryakov
Institute of Experimental Medicine of the North-West Branch of the Russian Academy of Medical Sciences
Email: kokryak@yandex.ru
References
- Кокряков В. Н. Очерки о врожденном иммунитете.- СПб.: Наука, 2006.- 262 с.
- Boman H. G. Peptide antibiotics and their role in innate immunity // Annu. Rev. Immunol.- 1995.- Vol. 13.- P. 61-92.
- Кокряков В. Н., Алешина Г. М., Шамова О. В. и др. Современная концепция об антимикробных пептидах как молекулярных факторах иммунитета // Медицинский академический журнал.- 2010.- Т. 10.- С. 149-160.
- Lehrer R. I. Evolution of antimicrobial peptides: a view from the cystine chapel // Antimicrobial Peptides and Innate Immunity, Progress in Inflammation Research / P. S. Hiemstra and S. A. J. Zaat (eds.) - Springer Basel, 2013.- P. 1-28.
- Pleskach V. A., Aleshina G. M., Artsybasheva I. V. et al. Cytotoxic and mitogenic effect of antimicrobial peptides from neutrophils on cultured cells // Цитология.- 2000.- Т. 42, № 3.- С. 228-234.
- Корнева Е. А., Шхинек Э. К. Гормоны и иммунная система.- Л.: Наука, 1988.- 251 с.
- Webster J. I., Tonelli L., Sternberg E. M. Neuroendocrine regulation of immunity // Annu. Rev. Immunol.- 2002.- Vol. 20.- P. 125-163.
- Khanfer R., Phillips A. C., Carroll D., Lord J. M. Altered human neutrophil function in response to acute psychological stress // Psychosomatic Medicine.- 2010.- Vol. 72.- P. 636-640.
- Шамова О. В., Лесникова М. П., Кокряков В. Н. и др. Действие дефенсинов на уровень кортикостерона в крови и иммунный ответ при стрессе // Бюлл. экспер. биол. и мед.- 1993.- Т. 115, № 6.- С. 646-649.
- Cervini L. A., Gray W. R., Kaiser R. et al. Rat corticostatin R4: synthesis, disulfide bridge assignment, and in vivo activity // Peptides.- 1995.- Vol. 16.- P. 837-842.
- Цветкова Е. В., Алешина Г. М., Шамова О. В. и др. -Дефенсины из лейкоцитов крови обезьяны Papio hamadryas // Биохимия.- 2006.- Т. 71, вып. 8.- С. 1083-1090.
- Орлов Д. С. Эффекты действия дефенсинов при стрессе: автореф. дис.. канд. биол. наук.- СПб., 1998.- 20 с.
- Горизонтов П. Д. Стресс. Система крови в механизме гомеостаза. Стресс и болезни // Гомеостаз / Под ред. П. Д. Горизонтова.- М.: Медицина, 1981.- C. 538-570.
- Davis J. M., Albert J. D., Tracy K. J. et al. Increased neutrophil mobilization and decreased chemotaxis during cortisol and epinephrine infusions // Journal of Trauma and Acute Care Surgery.- 1991.- Vol. 31.- P. 725-731.
- Dhabhar F. S., Malarkey W. B., Neri E., McEwen B. S. Stress-induced redistribution of immune cells - from barracks to boulevards to battlefields: a tale of three hormones // Psychoneuroendocrinology.- 2012.- Vol. 37.- P. 1345-1368.
- Parks K. R., Davis J. M. Epinephrine, cortisol, endotoxin, nutrition, and the neutrophil // Surg Infect (Larchmt).- 2012.- Vol. 13.- P. 300-306.
- Solomon S. Corticostatins // Trends Endocrinol Metab.- 1993.- Vol. 4.- P. 260-264.
- Terlau H., Olivera B. M. Conus venoms: a rich source of novel ion channel-targeted peptides // Physiol. Rev.- 2004.- Vol. 84.- P. 41-68.
- Плахова В. Б., Рогачевский И. В., Щееголев Б. Ф. и др. Дефенсин NP-4 уменьшает потенциалочувствительность медленных натриевых каналов сенсорных нейронов // Сенсорные системы.- 2005.- Т. 19, № 2.- С. 123-131.