OVARIAN AROMATASE P450 IN POLYCYSTIC OVARY SYNDROME



Cite item

Full Text

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription or Fee Access

Abstract

The objective of this study was the analysis the role of ovarian aromatase P450 in the pathogenesis of polycystic ovary syndrome. The study included 30 patients with normogonadotropic anovulation due to polycystic ovary syndrome (PCOS). The comparison group included 29 women with intact ovulatory menstrual cycle. The expression of aromatase р450 by ovarian granulose cells was determined using immunohistochemical method by 10-12 day menstrual cycle, and determination of total aromatase activity using letrozole probe on day 2-3 of the menstrual cycle. Deficiency of ovarian aromatase in the PCOS was associated with a significantly smaller maximal follicle, while the total aromatase activity does not exceed of physiological limits in healthy women. Results relate ovarian aromatase deficiency characteristic of PCOS with the regulatory disruption, including hyperproduction of luteinizing hormone by the pituitary gland

Full Text

Restricted Access

About the authors

V A Savina

Ott’s Institute of Obstetrics and Gynecology Russian Academy of Medical Sciences

Email: vasavina@mail.ru
St.-Petersburg, Russia

I M Kvetnoy

Ott’s Institute of Obstetrics and Gynecology Russian Academy of Medical Sciences

St.-Petersburg, Russia

M A Kleshchev

Ott’s Institute of Obstetrics and Gynecology Russian Academy of Medical Sciences

St.-Petersburg, Russia

V V Poteen

Ott’s Institute of Obstetrics and Gynecology Russian Academy of Medical Sciences

St.-Petersburg, Russia

V V Rulev

Ott’s Institute of Obstetrics and Gynecology Russian Academy of Medical Sciences

St.-Petersburg, Russia

M A Tarasova

Ott’s Institute of Obstetrics and Gynecology Russian Academy of Medical Sciences

St.-Petersburg, Russia

N N Tkachenko

Ott’s Institute of Obstetrics and Gynecology Russian Academy of Medical Sciences

St.-Petersburg, Russia

M I Jarmolinskaya

Ott’s Institute of Obstetrics and Gynecology Russian Academy of Medical Sciences

St.-Petersburg, Russia

References

  1. Knochenhauer E. S., Key T. J., Kahsar-Miller M. et al. Prevalence of the polycystic ovary syndrome in unselected black and white women of the southeastern United States: a prospective study // J. Clin. Endocrinol. Metab.- 1998.- Vol. 83, № 9.- P. 3078-3082.
  2. Michelmore K. F., Balen A. H., Dunger D. B. et al. Polycystic ovaries and associated clinical and biochemical features in young women // Clin. Endocrinol. (Oxf).- 1999.- Vol. 51, № 6.- P.779-786.
  3. Гинекология от пубертата до постменопаузы: практическое руководство для врачей / Э. К. Айламазян и др.- 2-е изд.- М.: МЕДпресс-информ, 2006.- C. 147-152.
  4. Назаренко Т. А., Дмитриев Д. В. Ингибиторы ароматазы в репродуктивной медицине // Проблемы репродукции.- 2007.- № 1.-С. 14-20.
  5. Simpson E. R. Biology of aromatase in the mammary gland // J. Mammary Gland Biol. Neoplasia.- 2000.- Vol. 5, № 3.- P. 251-258.
  6. Toda K., Simpson E. R., Mendelson C. R. et al. Expression of the gene encoding aromatase cytochrome P450 (CYP19) in fetal tissues // Mol. Endocrinol.- 1994.- Vol. 8, № 2.- P. 210-217.
  7. Вихляева Е. М. Синдром поликистозных яичников // Акуш. и гин.- 1973.- № 3.- C. 54-58.
  8. Erickson G. F., Hsueh A. J., Quigley M. E. et al. Functional studies of aromatase activity in human granulosa cells from normal and polycystic ovaries // J. Clin. Endocrinol. Metab.- 1979.- Vol. 49, № 4.- P. 514-519.
  9. Short R. V., London D. R. Defective Biosynthesis of Ovarian Steroids in the Stein-Leventhal Syndrome // Br. Med. J.- 1961.- Vol. 17, № 1.- P. 1724-1727.
  10. Deladoey J., Fluck C., Bex M. et al. Aromatase deficiency caused by a novel P450arom gene mutation: impact of absent estrogen production on serum gonadotropin concentration in a boy // J. Clin. Endocrinol. Metab.- 1999.- Vol. 84, № 11.- P. 4050-4054.
  11. Harada N., Ogawa H., Shozu M. et al. Biochemical and molecular genetic analyses on placental aromatase (P450arom) deficiency // J. Biol. Chem.- 1992.- Vol. 267, № 7.- P. 4781-4785.
  12. Ludwig M., Beck A., Wickert L. et al. Female pseudohermaphroditism associated with a novel homozygous G-to-A (V370-to-M) substitution in the P-450 aromatase gene // J. Pediatr. Endocrinol. Metab.- 1998.- Vol. 11, № 5.- P. 657-664.
  13. Portrat-Doyen S., Forest M. G., Nicolino M. et al. Female pseudohermaphroditism (FHP) resulting from aromatase (P450arom) deficiency associated with a novel mutation (R457) in the CYP19 gene // Horm. Res.- 1996.- Vol. 46.- P. 14-20.
  14. Shozu M., Akasofu K., Harada T. et al. A new cause of female pseudohermaphroditism: placental aromatase deficiency // J. Clin. Endocrinol. Metab.- 1991.- Vol. 72, № 3.- P. 560-566.
  15. Gougeon A. Regulation of Ovarian Follicular Development in Primates: Facts and Hypotheses // End. Rew.- 1996.- Vol. 17, № 2.-P. 121-155.
  16. McNatty K. P., Hunter W. M., MacNeilly A. S. et al. Changes in the concentration of pituitary and steroid hormones in the follicular fluid of human graafian follicles throughout the menstrual cycle // J. Endocrinol.- 1975.- Vol. 64, № 3.- P. 555-571.
  17. Suzuki T., Sasano H., Sasaki H. et al. Quantitation of P450 aromatase immunoreactivity in human ovary during the menstrual cycle: relationship between the enzyme activity and immunointensity // J. Histochem. Cytochem.- 1994.- Vol. 42, № 12.- P. 1565-1573.
  18. Pigny P., Merlen E., Robert Y. et al. Elevated serum level of anti-mullerian hormone in patients with polycystic ovary syndrome: relationship to the ovarian follicle excess and to the follicular arrest // Clin. Endocrinol. Metab.- 2003.- Vol. 88, № 12.- P. 5957-5962.
  19. Морчиладзе А. З., Савина В. А., Ткаченко Н. Н. и др. Применение ингибитора ароматазы летрозола для индукции овуляции у женщин с синдромом поликистозных яичников // Журнал акушерства и женских болезней.- 2011.- Т. LX, вып. 2.- С. 52-58.
  20. Бебия З. Н., Орлов В. М. Возможная роль гиперпродукции яичниками антимюллеровского фактора в патогенезе хронической ановуляции при синдроме поликистозных яичников // Проблемы репродукции.- 2000.- № 6.- С. 12-15.
  21. Pellatt L., Rice S., Mason H. D. Anti-Mullerian hormone and polycystic ovary syndrome: a mountain too high? // Reproduction.- 2010.-Vol. 139, №5.- P. 825-833.
  22. Pellatt L., Rice S., Dilaver N. et al. Anti-Mullerian hormone reduces follicle sensitivity to follicle-stimulating hormone in human granulosa cells // Fertil. Steril.- 2011.- Vol. 96, № 5.- P. 1246-1251.
  23. Pache T. D., Wladimiroff J. W., de Jong F. H. et al. Growth Patterns of Nondominant Ovarian Follicles during the Normal Menstrual Cycle // Fertil. Steril.- 1990.- Vol. 54, № 4.- P. 638-642.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2012 Savina V.A., Kvetnoy I.M., Kleshchev M.A., Poteen V.V., Rulev V.V., Tarasova M.A., Tkachenko N.N., Jarmolinskaya M.I.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: серия ПИ № ФС 77 - 74760 от 29.12.2018 г.


This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies