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Diurnal dynamics of rat blood electrolytes at constant lighting and chronic alcohol intoxication
- Authors: Kirillov Y.A.1, Makartseva L.A.1, Kozlova M.A.1, Muratova M.V.1, Chernov I.A.2, Areshidze D.A.1
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Affiliations:
- Petrovsky National Research Centre of Surgery
- Tyumen State Medical University
- Issue: Vol 22, No 1 (2022)
- Pages: 51-59
- Section: Original research
- URL: https://journals.eco-vector.com/MAJ/article/view/105272
- DOI: https://doi.org/10.17816/MAJ105272
- ID: 105272
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Abstract
BACKGROUND: Violation of the lighting regime, as well as alcohol abuse, is significant factors in the disorganization of biorhythms in the modern world. Violation of rhythm leads to a mismatch of body functions, as a result to disadaptation, the occurrence of desynchronosis and the development of a number of socially significant diseases.
AIM: Study of influence of constant lighting, chronic alcohol intoxication and joint action of these factors on diurnal dynamics of several electrolytes in blood of rats.
MATERIALS AND METHODS: The study was conducted on 160 male of Wistar outbred stock at age of 6 months, with body weight of 300 ± 20 g, which were divided into 4 groups. Control group — animals, which were kept under conditions of fixed light regime (light:darkness = 10:14 hours), I group — animals, which were kept under conditions of fixed light regime, but received a 15% water solution of ethanol as a drink, II group — animals, which were kept under conditions of constant light regime, III group — animals, which were kept under conditions of constant light regime and received a 15% water solution of ethanol as a drink. In blood samples, taken at 9:00, 15:00, 21:00 and 3:00 o’clock, the concentration of total calcium, potassium, sodium, chlorine and phosphorus was measured. The reliability of circadian rhythmicity was determined through cosinor analysis.
RESULTS: The increase in the concentration of phosphorus in the blood plasma of animals of II and III experimental groups was found with the decrease in the concentration of total calcium in animals of I and III experimental groups. Constant lighting and chronic alcohol intoxication affect the amplitude-phase characteristics of the circadian rhythms of all studied ions, and the combined action of these factors causes the destruction of the circadian rhythms of the studied electrolytes.
CONCLUSIONS: It is established that the three-weeks-long influence of the studied chronodestructors leads to disruptions in metabolism of calcium and phosphorus, which are the most pronounced at joint action of darkness deprivation and chronic alcohol intoxication, and also to the significant disruptions in the structure of CRs of all of studied substances.
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About the authors
Yuri A. Kirillov
Petrovsky National Research Centre of Surgery
Author for correspondence.
Email: youri_kirillov@mail.ru
ORCID iD: 0000-0003-3555-0902
SPIN-code: 6514-5577
Scopus Author ID: 56531783200
ResearcherId: AAE-7630-2021
MD, Dr. Sci. (Med.), Professor, Leading Researcher of Laboratory of Clinical Morphology, Avtsyn Research Institute of Human Morphology
Russian Federation, MoscowLyudmila A. Makartseva
Petrovsky National Research Centre of Surgery
Email: la.makartseva@outlook.com
ORCID iD: 0000-0002-1882-8848
SPIN-code: 4254-1571
Scopus Author ID: 57201418859
ResearcherId: AAE-5136-2021
Junior Researcher of Laboratory of Pathology of Cell, Avtsyn Research Institute of Human Morphology
Russian Federation, MoscowMaria A. Kozlova
Petrovsky National Research Centre of Surgery
Email: ma.kozlova2021@outlook.com
ORCID iD: 0000-0001-6251-2560
SPIN-code: 5647-1372
Scopus Author ID: 55976515700
ResearcherId: AAE-5096-2021
Researcher of Laboratory of Pathology of Cell, Avtsyn Research Institute of Human Morphology
Russian Federation, MoscowMarina V. Muratova
Petrovsky National Research Centre of Surgery
Email: marymurvl@gmail.com
ORCID iD: 0000-0003-1183-1398
Resident, Avtsyn Research Institute of Human Morphology
Russian Federation, MoscowIgor A. Chernov
Tyumen State Medical University
Email: chernov@tyumsmu.ru
ORCID iD: 0000-0002-6475-5731
SPIN-code: 4674-8531
Scopus Author ID: 12778132200
ResearcherId: Z-5145-2019
MD, Cand. Sci. (Med.), Assistant Professor, Head of the Department of Pathological Anatomy and Forensic Medicine
Russian Federation, TyumenDavid A. Areshidze
Petrovsky National Research Centre of Surgery
Email: labcelpat@mail.ru
ORCID iD: 0000-0003-3006-6281
SPIN-code: 4348-6781
Scopus Author ID: 55929152900
ResearcherId: G-8387-2014
Cand. Sci. (Biol.), Leading Research Associate, Head of Laboratory of Pathology of Cell. Avtsyn Research Institute of Human Morphology
Russian Federation, MoscowReferences
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СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).