COMBINED ACTION OF ANTIMICROBIAL PEPTIDES AND NONIONIC SURFACTANTS TOWARDS BACTERIA

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Abstract

Antimicrobial peptides (AMPs) have certain resemblance with surfactants. Using checkerboard titrations and spectrophotometrical analysis of the membrane permeability for chromogenic markers, we found that nonionic surfactants “Tritons” significantly increase the speed of membranolytic action of AMPs, and enhance their antibacterial activity.

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M S Zharkova

Institute of Experimental Medicine, Saint Petersburg

D S Orlov

Institute of Experimental Medicine, Saint Petersburg

A A Kolobov

State Research Institute of Highly Pure Biopreparations, Saint Petersburg

O V Shamova

Institute of Experimental Medicine, Saint Petersburg

References

  1. Antibacterial peptide protocols / W. Shafer (ed.). Totowa, NJ: Humana Press Inc., 1998. 255 p.
  2. Orhan G, et al. Synergy Tests by E Test and Checkerboard Methods of Antimicrobial Combinations against Brucella melitensis. J. Clin. Microbiol. 2005;43(1):140-143.
  3. Lehrer R, et al. Concurrent assessment of inner and outer membrane permeabilization and bacteriolysis in E. coli by multiple-wavelength spectrophotometry. J. Immunol. Methods. 1988;108:153-158.

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Copyright (c) 2019 Zharkova M.S., Orlov D.S., Kolobov A.A., Shamova O.V.

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