MORPHOLOGICAL ASSESSMENT OF THE OVARIANS AFTER SINGLE AND FRACTIONAL LOCAL ELECTRON IRADIATION
- Authors: Demyashkin G.1,2, Murtazalieva Z.1, Vadyukhin M.1, Bimurzaeva M.1, Lotyrov M.1
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Affiliations:
- Sechenov University
- National Research Center for Radiology
- Pages: 43-52
- Section: Original research
- URL: https://journals.eco-vector.com/MAJ/article/view/567961
- DOI: https://doi.org/10.17816/MAJ567961
- ID: 567961
Cite item
Abstract
When malignant neoplasms of the pelvic organs are irradiated, healthy ovarian tissues can get into the irradiation area. So, among all physico-chemical factors, ionizing radiation is the most common cause of ovarian failure, which has a negative impact on fertility. Conducting research in this area is especially important in connection with the active introduction of electron therapy in the protocols for the treatment of malignant neoplasms of the small pelvis with the need to find ways to prevent and treat post-radiation ovarian lesions. In addition, one of the main tasks of modern radiobiology is the creation of experimental animal models in order to reveal the mechanisms of radiation exposure with subsequent extrapolation of the results obtained to humans in order to level the side effects of radiation therapy and select optimal doses.
The aim of the study was a morphofunctional assessment of the ovaries after local electron irradiation in single and fractional modes.
Materials and methods. Wistar rats (n=30) were divided into three groups: I – control (n=10); II (n=10) – subjected to a single local irradiation with electrons at a dose of 2 Gy; III (n=10) – subjected to fractional local irradiation with electrons in a total dose of 20 Gy (in daily fractions of 5 Gy).
Results. After a single local irradiation with electrons at a dose of 2 Gy, multiple hemorrhages and a decrease in the number of growing follicles with a discontinuous theca layer, which were unevenly distributed over its volume, were noted in the ovary. A statistically significant difference in the number of follicles was revealed: a decrease in the number of primordial, primary, secondary and tertiary follicles and an increase in atretic follicles. The most pronounced difference in the number of follicles between the studied groups was found in the group of fractional electron irradiation at a dose of 20 Gy: the smallest number of primordial and the largest number of atretic follicles with signs of post-radiation fibrosis.
Conclusion. The most profound damage to the ovary develops after exposure to fractional electron irradiation at a total dose of 20 Gy compared with a single exposure to ionizing radiation at a dose of 2 Gy: a reduced number of follicles, a decrease in the area and thickness of the cortical substance, as well as the thickness of the ovarian tunica, in combination with the growth of the connective tissue.
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About the authors
Grigory Demyashkin
Sechenov University; National Research Center for Radiology
Author for correspondence.
Email: dr.grigdem@gmail.com
ORCID iD: 0000-0001-8447-2600
cand. med. sci., Head of the Laboratory of Histology and Immunohistochemistry, ITMiB, Sechenov University; Head of the Department of Pathomorphology, National Research Center for Radiology
Russian Federation, 119991, Moscow, st. Trubetskaya, d. 8, building 2; 125284, Moscow, 2nd Botkinsky pr., 3Zaira Murtazalieva
Sechenov University
Email: ZARIA.ALIEVA.90@BK.RU
ORCID iD: 0009-0000-2361-7618
post-graduate student
Russian Federation, 119991, Moscow, st. Trubetskaya, d. 8, building 2Matvey Vadyukhin
Sechenov University
Email: vma20@mail.ru
ORCID iD: 0000-0002-6235-1020
6th year student of the Institute of Clinical Medicine
Russian Federation, 119991, Moscow, st. Trubetskaya, d. 8, building 2Makka Bimurzaeva
Sechenov University
Email: bimakka@mail.ru
ORCID iD: 0000-0002-3065-0755
6th year student of the Institute of Clinical Medicine
Russian Federation, 119991, Moscow, st. Trubetskaya, d. 8, building 2Magomed Lotyrov
Sechenov University
Email: lotyrov_m_i@student.sechenov.ru
6th year student of the Institute of Clinical Medicine
Russian Federation, 119991, Moscow, st. Trubetskaya, d. 8, building 2References
- Gerbi BJ, Antolak JA, Deibel FC, Followill DS, Herman MG, Higgins PD, Huq MS, Mihailidis DN, Yorke ED, Hogstrom KR, et al. Recommendations for clinical electron beam dosimetry: Supplement to the recommendations of Task Group 25. Med. Phys. 2009;36:3239. https://doi.org/10.1118/1.3125820
- Reiser E, Bazzano MV, Solano ME, Haybaeck J, Schatz C, Mangesius J, Ganswindt U, Toth B. Unlaid Eggs: Ovarian Damage after Low-Dose Radiation. Cells. 2022;11(7):1219. https://doi.org/10.3390/cells11071219
- Immediata V, Ronchetti C, Spadaro D, Cirillo F, Levi-Setti PE. Oxidative Stress and Human Ovarian Response-From Somatic Ovarian Cells to Oocytes Damage: A Clinical Comprehensive Narrative Review. Antioxidants (Basel). 2022;11(7):1335. https://doi.org/10.3390/antiox11071335
- Radford IR. DNA lesion complexity and induction of apoptosis by ionizing radiation. Int J Radiat Biol. 2002;78(6):457-66. https://doi.org/10.1080/09553000110120337
- Lee CJ, Yoon Y. Gamma-radiation-induced follicular degeneration in the prepubertal mouse ovary. Mutat Res. 2005;578(2):247–255. https://doi.org/10.1016/j.mrfmmm.2005.05.019
- Reisz JA, Bansal N, Qian J, Zhao W, Furdui CM. Effects of ionizing radiation on biological molecules--mechanisms of damage and emerging methods of detection. Antioxid Redox Signal. 2014;21(2):260-92. https://doi.org/10.1089/ars.2013.5489
- Boots C, Jungheim E. Inflammation and human ovarian follicular dynamics. Semin Reprod Med. 2015;33(4):270–275. https://doi.org/10.1055/s-0035-1554928
- Grover AR, Kimler BF, Duncan FE. Use of a Small Animal Radiation Research Platform (SARRP) facilitates analysis of systemic versus targeted radiation effects in the mouse ovary. J Ovarian Res. 2018;11(1):72. https://doi.org/10.1186/s13048-018-0442-8
- England K, Cotter TG. Direct oxidative modifications of signalling proteins in mammalian cells and their effects on apoptosis. Redox Rep. 2005;10(5):237–245. https://doi.org/10.1179/135100005X70224
- Meirow D, Nugent D. The effects of radiotherapy and chemotherapy on female reproduction. Hum Reprod Update. 2001;7(6):535-43. https://doi.org/10.1093/humupd/7.6.535
- Oktem O, Kim SS, Selek U, Schatmann G, Urman B. Ovarian and Uterine Functions in Female Survivors of Childhood Cancers. Oncologist. 2018;23(2):214-224. https://doi.org/10.1634/theoncologist.2017-0201
- Gao W, Liang JX, Ma C, Dong JY, Yan Q. The Protective Effect of N-Acetylcysteine on Ionizing Radiation Induced Ovarian Failure and Loss of Ovarian Reserve in Female Mouse. Biomed Res Int. 2017;2017:4176170. https://doi.org/10.1155/2017/4176170
- Alesi LR, Nguyen QN, Stringer JM, Winship AL, Hutt KJ. The future of fertility preservation for women treated with chemotherapy. Reprod Fertil. 2023;4(2):e220123. https://doi.org/10.1530/RAF-22-0123
- Zhang S, Liu Q, Chang M, Pan Y, Yahaya BH, Liu Y, Lin J. Chemotherapy impairs ovarian function through excessive ROS-induced ferroptosis. Cell Death Dis. 2023;14(5):340. https://doi.org/10.1038/s41419-023-05859-0
- Meirow D, Biederman H, Anderson RA, Wallace WH. Toxicity of chemotherapy and radiation on female reproduction. Clin Obstet Gynecol. 2010;53:727–739. https://doi.org/10.1097/GRF.0b013e3181f96b54
- Taskin MI, Yay A, Adali E, Balcioglu E, Inceboz U. Protective effects of sildenafil citrate administration on cisplatin-induced ovarian damage in rats. Gynecol Endocrinol. 2015;31(4):272-7. https://doi.org/10.3109/09513590.2014.984679
- Land KL, Miller FG, Fugate AC, Hannon PR. The effects of endocrine-disrupting chemicals on ovarian- and ovulation-related fertility outcomes. Mol Reprod Dev. 2022;89(12):608-631. https://doi.org/10.1002/mrd.23652
- Kim S, Kim SW, Han SJ, Lee S, Park HT, Song JY, Kim T. Molecular Mechanism and Prevention Strategy of Chemotherapy- and Radiotherapy-Induced Ovarian Damage. Int J Mol Sci. 2021;22(14):7484. https://doi.org/10.3390/ijms22147484
- Devine PJ, Perreault SD, Luderer U. Roles of reactive oxygen species and antioxidants in ovarian toxicity. Biol Reprod. 2012;86(2):27. https://doi.org/10.1095/biolreprod.111.095224
- Wang W, Craig ZR, Basavarajappa MS, Gupta RK, Flaws JA. Di (2-ethylhexyl phthalate inhibits growth of mouse ovarian antral follicles through an oxidative stress pathway. Toxicology and Applied Pharmacology. 2012;258(2):288–295. https://doi.org/10.1016/j.taap.2011.11.008
- Yan F, Zhao Q, Li Y, Zheng Z, Kong X, Shu C, Liu Y, Shi Y. The role of oxidative stress in ovarian aging: a review. J Ovarian Res. 2022;15(1):100. https://doi.org/10.1186/s13048-022-01032-x
- Rudnicka E, Kunicki M, Calik-Ksepka A, Suchta K, Duszewska A, Smolarczyk K, Smolarczyk R. Anti-Müllerian Hormone in Pathogenesis, Diagnostic and Treatment of PCOS. Int J Mol Sci. 2021;22(22):12507. https://doi.org/10.3390/ijms222212507
- Chatziandreou E, Eustathiou A, Augoulea A, Armeni E, Mili N, Boutas I, Tsoltos N, Kapetanaki A, Kalantaridou S. Antimüllerian Hormone as a Tool to Predict the Age at Menopause. Geriatrics (Basel). 2023;8(3):57. https://doi.org/10.3390/geriatrics8030057
- Onder GO, Balcioglu E, Baran M, Ceyhan A, Cengiz O, Suna PA, Yıldız OG, Yay A. The different doses of radiation therapy-induced damage to the ovarian environment in rats. Int J Radiat Biol. 2021;97(3):367-375. https://doi.org/10.1080/09553002.2021.1864497