The correlation of morphometric and biochemical parameters in regenerating tissues of skin burn wounds in rats at assession the reparative properties of 2-ethyl-6-methyl-3-hydroxypyridinium N-acetyl-6-aminohexanoate

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Abstract

BACKGROUND: Aminohexanoic acid and its derivatives are potential reparants. The study is dedicated to the assessment of the aminohexanoic acid derivative on the healing of experimental thermal skin burns.

AIM: To study the relationship between morphometric and biochemical parameters during an experiment to evaluate the effectiveness of 2% ointment with 2-ethyl-6-methyl-3-hydroxypyridinium N-acetyl-6-aminohexanoate for the treatment of skin burns in rats.

MATERIALS AND METHODS: The thermal skin burns of 25 mm2 area was modeled in 63 white non-linear sexually mature female rats under general anesthesia with Zoletil 100. The rats were divided into three groups depending on the local effect on the burn area: the control 1 group of rats — with imitation of application procedure without applying substances; the control 2 group with application of an ointment base (polyethylene glycol); the experimental group with application of 2% ointment with 2-ethyl-6-methyl-3-hydroxypyridinium N-acetyl-6-aminohexanoate. Applications of ointment, ointment base and their imitation were carried out starting from the second day until the end of the experiment. Morphometric and biochemical parameters were studied in homogenates of regenerating tissues of skin wounds on 7, 14 and 21 experiment days.

RESULTS: In experimental group rats during the inflammation phase, the efficiency of regeneration, confirmed by the dynamics of the area wounds, was largely correlated with the width of the leukocyte shaft and the thickness of granulation tissue, which, in turn, depended on the levels of Tumor Necrosis Factor alfa and Vascular Endothelial Growth Factor, respectively. In the proliferation phase, the area of wounds was significantly correlated with the length of the epithelial wedge and the fibroblasts number in the field of view, which, respectively, depended on the levels of Transforming Growth Factor beta and basic Fibroblast Growth Factor.

CONCLUSIONS: Results of the correlation analysis have testified that the reparative effect of local exposure with 2% ointment with 2-ethyl-6-methyl-3-hydroxypyridinium N-acetyl-6-aminohexanoate is ensured by activation of the synthesis of growth factors that stimulate the proliferation of capillaries and fibroblasts — components of granulation tissue, the active formation of which induces epidermis repair, which confirmed by planimetric data.

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About the authors

Marina A. Petrovskaya

Tver State Medical University

Author for correspondence.
Email: solm1990@mail.ru
ORCID iD: 0000-0003-1193-1778
SPIN-code: 5512-7253

postgraduate student, assistant of the Department of Biology

Russian Federation, Tver

Margarita B. Petrova

Tver State Medical University

Email: pmargo-2612@mail.ru
ORCID iD: 0009-0004-7620-5958
SPIN-code: 4310-3839

Dr. Sci. (Biology), Professor, Head of the Department of Biology

Russian Federation, Tver

Elena N. Egorova

Tver State Medical University

Email: enegor@mail.ru
ORCID iD: 0000-0002-4323-5286
SPIN-code: 5805-8780

MD, Dr. Sci. (Medicine), Assistant Professor, Head of the Department of Biochemistry with a course of clinical laboratory diagnostics

Russian Federation, Tver

Elena V. Andrianova

Tver State Medical University

Email: andrianovaalenav@mail.ru
ORCID iD: 0009-0000-5825-7317
SPIN-code: 3946-9969

Cand. Sci. (Biology), Assistant of the Department of Biochemistry with a course of clinical laboratory diagnostics

Russian Federation, Tver

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Supplementary files

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2. Fig. 1. Dynamics of reduction of the skin defects area in rats during the regeneration of thermal burns. * The difference is statistically significant (р < 0.05) between the indicator in animals of the experimental group and control groups 1 and 2

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3. Fig. 2. Morphological picture of granulation tissue in rats of the control 1 (a) and experimental groups (b) on day 7 wounds. H&E staining. ×400

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4. Fig. 3. Morphological picture of lesion area in rats of control 1 group (a) and experimental group (b) on day 14 wounds. H&E staining. ×400

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