Changes in the activity of amylase, alkaline phosphatase and lipase in placenta and umbilical cord blood in women in labor with gestational diabetes mellitus

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Abstract

BACKGROUND: The prevalence of gestational diabetes mellitus in the world is increasing every year. However, there is a small number of studies on changes in enzymes of various classes in blood serum in gestational diabetes mellitus, and there are conflicting results regarding the concentration of individual enzymes.

AIM: To study the activity of amylase, alkaline phosphatase and lipase in placenta and umbilical cord blood in women in labor with gestational diabetes mellitus in comparison with women without this pathology.

METHODS: Cord blood and placenta sections were obtained from women with (n = 20) and without (n = 62) gestational diabetes mellitus during labor in the presence of informed consent. Alkaline phosphatase and lipase activity was estimated by kinetic colorimetric method, amylase activity by amyloclastic method according to Caraway.

RESULTS: It has been shown that in pregnant women with gestational diabetes mellitus who are on diet therapy, significant differences in the activity of amylase and alkaline phosphatase are present in the homogenate of the placenta and umbilical cord blood compared with pregnant women without gestational diabetes mellitus. In pregnant women without gestational diabetes mellitus and women on insulin therapy, no significant differences were found in the studied fluids according to these enzymes. There were no significant differences in the lipolytic activity of umbilical cord blood and placental homogenate between the groups of women studied.

CONCLUSION: The study showed that gestational diabetes mellitus in women leads to significant changes in the activity of enzymes in the homogenate of the placenta and umbilical cord blood.

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About the authors

Elena V. Kolodkina

Saint Petersburg State Pediatric Medical University; Almazov National Medical Research Center

Email: 922-666-2045@mail.ru
ORCID iD: 0009-0001-6304-8680
SPIN-code: 9082-3341

MD, Cand. Sci. (Medicine), Associate Professor at the Department of Normal Physiology; Associate Professor at the Department of Pathological Physiology

Russian Federation, Saint Petersburg; Saint Petersburg

Sergey A. Lytaev

Saint Petersburg State Pediatric Medical University

Email: lytaev_sergei@mail.ru
ORCID iD: 0000-0002-1291-0381
SPIN-code: 5100-5552

MD, Dr. Sci. (Medicine), Professor, Head of the Department of Normal Physiology

Russian Federation, Saint Petersburg

Michael M. Galagudza

Almazov National Medical Research Center

Email: galagudza@almazovcentre.ru
ORCID iD: 0000-0001-5129-9944
SPIN-code: 2485-4176

MD, Dr. Sci. (Medicine), Professor, Corresponding Member of the RAS, Director of the Institute of Experimental Medicine, Head of the Department of Pathophysiology

Russian Federation, Saint Petersburg

Alexander B. Loginov

Professor Snegirev Maternity Hospital No. 6

Email: log15@yandex.ru

MD, Cand. Sci. (Medicine), Deputy Chief Medical Officer

Russian Federation, Saint Petersburg

Tatyana M. Pervunina

Almazov National Medical Research Center

Email: pervunina_tm@almazovcentre.ru
ORCID iD: 0000-0001-9948-7303
SPIN-code: 3288-4986

MD, Dr. Sci. (Medicine), Director of the Institute of Perinatology and Pediatrics, Head of the Department of Perinatology and Pediatrics of the Institute of Medical Education

Russian Federation, Saint Petersburg

Stanislav I. Sitkin

Almazov National Medical Research Center

Email: drsitkin@gmail.com
ORCID iD: 0000-0003-0331-0963
SPIN-code: 3961-8815

MD, Cand. Sci. (Medicine), Head of the Institute of Epigenetics and Metagenomics of the Institute of Perinatology and Pediatrics

Russian Federation, Saint Petersburg

Yuri Yu. Borschchev

Almazov National Medical Research Center; Petrov National Medical Research Center of Oncology

Email: niscon@mail.ru
ORCID iD: 0000-0003-3096-9747
SPIN-code: 3454-4113

Сand. Sci. (Biology), Head of the Toxicology Department of the Institute of Experimental Medicine; Researcher at the Laboratory of Cancer Chemoprophylaxis and Oncopharmacology

Russian Federation, Saint Petersburg; Saint Petersburg

Olga V. Borschcheva

Almazov National Medical Research Center

Author for correspondence.
Email: violga27@mail.ru
ORCID iD: 0009-0007-6131-3085
SPIN-code: 7532-5404

Researcher at the Toxicology Department of the Institute of Experimental Medicine

Russian Federation, Saint Petersburg

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Supplementary files

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2. Fig. 1. Amylase activity in umbilical cord blood serum and placental homogenate in pregnant women with and without gestational diabetes mellitus.

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3. Fig. 2. Alkaline phosphatase activity in umbilical cord blood serum and placental homogenate in pregnant women with and without gestational diabetes mellitus.

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4. Fig. 3. Lipase activity in umbilical cord blood serum and placental homogenate in pregnant women with and without gestational diabetes mellitus.

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5. Fig. 4. Enzyme activity in pregnant women with gestational diabetes mellitus undergoing diet therapy, in placental homogenate (A1), in cord blood serum (A2); in pregnant women without gestational diabetes, in placental homogenate (B1), in cord blood serum (B2).

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6. Fig. 5. Enzyme activity in pregnant women with gestational diabetes mellitus undergoing insulin therapy, in placental homogenate (A1), in cord blood serum (A2); in pregnant women without gestational diabetes, in placental homogenate (B1), in cord blood serum (B2).

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