Calpain expression associated with experimental allergic encephalomyelitis is increased in inflammatory cells and cells of CNS

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Abstract

Calpain is а system of Ca2+-activated neutral protease. Calpain is implicated in the pathophysiology of many disorders including multiply sclerosis and experimental allergic encephalomyelitis (ЕАЕ). At the present study, calpain expression at transcriptional levels was evaluated in splenic inflammatory cells and cells of spinal cord of Wistar rats with ЕАЕ prior to the onset of clinical symptoms. ln spleen of rats with ЕАЕ we established increasing m-and µ-calpain mRNA levels on the third day after induction of ЕАЕ comparison with normal control. Вy the 5th day we have found out the decrease calpain level in comparison with third day. This level was the same as control group. However by the 7th day calpain expression has been increased again. Calpain expression in spinal cords of animals with ЕАЕ was increased on day 3 and remained at а high level till 7th day. These studies suggest calpain may play а role in the inflammatory response associated with ЕАЕ in the spleen as well as in CNS.

About the authors

М. N. Karpenko

Research lnstitute of Experimental Medicine of the RAMS

Author for correspondence.
Email: medaj@eco-vector.com
Russian Federation, St. Petersburg

I. N. Abdurasulova

Research lnstitute of Experimental Medicine of the RAMS

Email: medaj@eco-vector.com
Russian Federation, St. Petersburg

Ur. L. Zjitnuchin

Research lnstitute of Experimental Medicine of the RAMS

Email: medaj@eco-vector.com
Russian Federation, St. Petersburg

V. М. Klimenko

Research lnstitute of Experimental Medicine of the RAMS

Email: medaj@eco-vector.com
Russian Federation, St. Petersburg

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Copyright (c) 2008 Karpenko М.N., Abdurasulova I.N., Zjitnuchin U.L., Klimenko V.М.


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