Investigating the effects of Inonotus rickii extracts on the muscle contraction intensity

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The aim of this study is to test the effect of aqueous, ethereal and alcoholic extracts of the fruit bodies of the wood-destroying fungus Inonotus rickii on locomotor activity resulting from contraction of both cross-striated and smooth muscles. The pharmacological activity of I. rickii raw materials was determined in vitro using the dose-response curve method (smooth muscles) and in experiments with oral intake of extracts (CNS-mediated effects on cross-lacing muscles). The aqueous extract of fungal material showed an increase in the motor activity of smooth muscles compared to standard caffeine, which indicates the ability of fungal extract to have a stimulating effect on the synapses. It was found that I. rickii extracts have an effect on smooth muscle contraction similar to the acetylcholine. It was shown that the greatest stimulating activity demonstrates an aqueous extract that may be a result of inhibitory effect of diethyl ether and ethanol on synapses. The described effects put on the agenda both the fractionation of active extracts and further experiments on the therapeutic applications of their described properties. As a field of possible application of this kind of substances can be considered the cardiovascular remodeling, the maintenance of smooth muscle tone during a number of surgical interventions, and the palliative cure of disseminated cancers.

About the authors

Kiran Ramchandra Ranadive

Department of Botany, P.D.E.A.’s Annasaheb Magar Mahavidyalaya, Mahadevnagar

Author for correspondence.
ORCID iD: 0000-0001-9673-7872
Scopus Author ID: 36629222300

M.Sc., Ph.D., Associate Professor, P.D.E.A.’s Annasaheb Magar Mahavidyalaya, Mahadevnagar

India, Hadapsar, Pune-411028

Neeta Vijayrao Jagtap

Department of Chemistry, P.D.E.A.’s Waghire College Saswad


M. Sci. and M. Phil., Assistant Professor

India, Taluka-Purandar, District-Pune, Pune-412301

Pradnya Nilesh Jagtap

Department of Pharmacology, P.D.E.A.’s SGRS College of Pharmacy, Saswad


M. Pharm., Assistant Professor, Department of Pharmacology, P.D.E.A.’s SGRS College of Pharmacy, Saswad, Taluka-Purandar

India, Taluka-Purandar, District-Pune, Pune-412301

Ivan V. Zmitrovich

Laboratory of Systematics and Geography of Fungi, Komarov Botanical Institute

ORCID iD: 0000-0002-3927-2527
SPIN-code: 4155-3190
ResearcherId: I-1523-2013

D.Sc. in Biology, Leading Researcher, Laboratory of Systematics and Geography of the Fungi, Komarov Botanical Institute RAS

Russian Federation, St. Petersburg

Vladimir V. Perelygin

Saint Petersburg State Chemical and Pharmaceutical University

ORCID iD: 0000-0002-0999-5644
SPIN-code: 3128-7451
ResearcherId: AAV-6556-2020

Doctor of Medicine (MD), Professor, Head of the Industrial Ecology Department, Saint Petersburg State Chemical Pharmaceutical University

Russian Federation, St. Petersburg


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Supplementary files

Supplementary Files
1. Fig. 1. Protocol for studying the range of contraction of the muscles of the chicken ileum (mm) under the action of a natural neurotransmitter (acetylcholine) and extracts of Inonotus rickii

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2. Fig. 2. Results of studying the CNS-mediated spontaneous locomotor activity under the influence of Inonotus rickii extracts: 1 – caffeine, 2 – diazepam, 3 – ether extract, 4 – ethanol extract, 5 – aqueous extract

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Copyright (c) 2021 Ranadive K.R., Jagtap N.V., Jagtap P.N., Zmitrovich I.V., Perelygin V.V.

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

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