Antihypoxic activity and anti-ischemic effect of complex herbal remedy

Cover Page

Cite item

Full Text

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription or Fee Access


The present study was carried out to determine the antihypoxic and anti-ischemic effects of a polyherbal extract from Astragalus membranaceus, Scutellaria baicalensis and Phlojodicarpus sibiricus. The Wistar rats of both sexes were used in the study. The antihypoxic activity of the extract was studied using hemic and hypobaric models of hypoxia. The anti-ischemic effect of the extract was evaluated by the severity of neurological deficit and survival within 24 hours in model of bilateral occlusion of the common carotid arteries.

The course administration of the extract (100, 200 mg/kg) had an antihypoxic effect in the model of hemic hypoxia: the reserve time of life increased by 26 and 28%, respectively, compared with the control. The course introduction of the extract at doses of 50, 100 and 200 mg/kg increased the reserve time of life in hypobaric hypoxia by 12.3, 15.5 and 16.7%, respectively, compared with the control

The administration of the extract (100 and 200 mg/kg) in the model of cerebral ischemia had a cerebroprotective effect: the indicators of neurological deficit were, respectively, less than the control by 34 and 46%, and their survival was 62 and 64%, respectively, against 20% of the survival of rats in the control group. The results of the conducted studies suggest the prospects for further study of the plant extract as a cerebroprojector agent.

Full Text

Restricted Access

About the authors

Sergey M. Gulyaev

Institute of General and Experimental Biology of the Siberian Branch of the Russian Academy of Sciences

ORCID iD: 0000-0001-8412-5799

Cand. Sci. (Med.), Senior Research Associate of the Laboratory of Experimental Pharmacology

Russian Federation, 6, Cakhyanovoi st., Ulan-Ude, 670047

Yuriy V. Zhalsanov

Regional Clinical Veteran Hospital


Chief, Department of Neurology

Russian Federation, 6, Cakhyanovoi st., Ulan-Ude, 670047

Tatiana A. Turtueva

Institute of General and Experimental Biology of the Siberian Branch of the Russian Academy of Sciences


Post-graduate Student

Russian Federation, 6, Cakhyanovoi st., Ulan-Ude, 670047

Galina G. Nikolaeva

Institute of General and Experimental Biology of the Siberian Branch of the Russian Academy of Sciences

Author for correspondence.

Dr. Sci. (Pharm.), Leading Research Associate

Russian Federation, 6, Cakhyanovoi st., Ulan-Ude, 670047


  1. Zhen Y, Shan Li Y, et al. Exploring the potential mechanism of radix astragali against ischemic stroke based on network pharmacology and molecular docking. Phytomedicine Plus. 2022;2(2):100244. doi: 10.1016/j.phyplu.2022.100244
  2. Xu Q, Bauer R, Hendry BM, et al. The quest for modernisation of traditional Chinese medicine. MC Complement Altern Med. 2013;13:132 doi: 10.1186/1472-6882-13-132
  3. Shen J, Li P, Liu S, et al. Traditional uses, ten-years research progress on phytochemistry and pharmacology, and clinical studies of the genus Scutellaria. J Ethnopharmacol. 2021;265:113198. doi: 10.1016/j.jep.2020.113198
  4. Wang F, Zhao S, Li F, et al. Investigation of antioxidant interactions between Radix Astragali and Cimicifuga foetida and identification of synergistic antioxidant compounds. Plos One. 2014;9(1): e87221.
  5. Fan CL, Cai WY, Ye MN, et al. Qili Qiangxin, a compound herbal medicine formula, alleviates hypoxia-reoxygenation-induced apoptotic and autophagic cell death via suppression of ROS/AMPK/mTOR pathway in vitro. J Integr Med. 2022;20(4):365–375. doi: 10.1016/j.joim.2022.04.005
  6. Wang D, Liu Y, Zhong G, et al. Compatibility of Tanshinone IIA and Astragaloside IV in attenuating hypoxia-induced cardiomyocytes injury. J Ethnopharmacol. 2017;204:67–76. doi: 10.1016/j.jep.2017.03.053
  7. Mc Graw СP. Experimental cerebral infarction: effect of pentobarbital in Mongolian gerbils. Arch Neurol. 1997;34(6):334–336. doi: 10.1001/archneur.1977.00500180028006
  8. Khoshnam SE, Winlow W, Farzaneh M, et al. Pathogenic mechanisms following ischemic stroke. Neurol Sci. 2017;38(7):1167–1186. doi: 10.1007/s10072-017-2938-1
  9. Turtueva TA. Razrabotka sbora neiroprotektivnogo i ekstrakta sukhogo na ego osnove [Dissertation]. Ulan-Ude; 2015. 23 p.
  10. Shen N, Wang T, Gan Q. Plant flavonoids: Classification, distribution, biosynthesis, and antioxidant activity. Food Chem. 2022;383:132531. doi: 10.1016/j.foodchem.2022.132531
  11. Jia JY, Zang EH, Lv LJ. Flavonoids in myocardial ischemia-reperfusion injury: Therapeutic effects and mechanisms. Chin Herb Med. 2021;13(1):49–63. doi: 10.1016/j.chmed.2020.09.002
  12. Sowndhararajan K, Deepa P, Kim M, et al. Baicalein as a potent neuroprotective agent: A review. Biomed Pharmacother. 2017;95:1021–1032. doi: 10.1016/j.biopha.2017.08.135
  13. Liang W, Huang X, Chen W. The Effects of Baicalin and Baicalein on Cerebral Ischemia: A Review. Aging Dis. 2017;8(6):850–867. doi: 10.14336/AD.2017.0829
  14. Park DJ, Kang JB, Shah FA, et al. Quercetin attenuates decrease of thioredoxin expression following focal cerebral ischemia and glutamate-induced neuronal cell damage. Neuroscience. 2020;428: 38–49. doi: 10.1016/j.neuroscience.2019.11.043
  15. Qin X, Qin H, Li Z, et al. Luteolin alleviates ischemia/reperfusion injury-induced no-reflow by regulating Wnt/β-catenin signaling in rats. Microvasc Res. 2022;139:104266. doi: 10.1016/j.mvr.2021.104266
  16. Dongmoa AB, Azebaze AGB, Nguelefack TB, et al. Vasodilator effect of the extracts and some coumarins from the stem bark of Mammea africana (Guttiferae). J Ethnopharmacol. 2007;111(2): 329–334. doi: 10.1016/j.jep.2006.11.026

Copyright (c) 2022 ECO-vector LLC

СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: серия ПИ № ФС 77 - 65565 от 04.05.2016 г.

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies