Effects of S-adenosyl methionine and essential phospholipids on serum biochemical parameters in simulated acute liver disease in late pregnancy
- Authors: Petrosyan M.A.1,2, Zhestkova N.V.1, Tumasova Z.N.1, Polyanskikh L.S.1, Balashova N.N.1, Baziian E.V.1, Kuz’minykh T.U.3, Razygraev A.V.1,4,5
-
Affiliations:
- D.O. Ott Institute of Obstetrics, Gynecology and Reproductolog
- Almazov National Medical Research Centre
- Saint Petersburg State University
- St. Petersburg State Chemical-Pharmaceutical University
- Zoological Institute Russian Academy of Sciences
- Issue: Vol 19, No 1 (2021)
- Pages: 65-70
- Section: Original articles
- Submitted: 14.05.2021
- Accepted: 14.05.2021
- Published: 21.05.2021
- URL: https://journals.eco-vector.com/RCF/article/view/70621
- DOI: https://doi.org/10.17816/RCF19165-70
- ID: 70621
Cite item
Abstract
AIM: Liver diseases, developing in late pregnancy, remains a very important problem in obstetrics. In present study, the aim was to evaluate the effectiveness of monotherapy by “essential phospholipids” and by S-adenosyl methionine for simulated acute liver pathology during pregnancy using the biochemical parameters of blood serum.
STUDY DESIGN AND METHODS: Acute liver injury was simulated in rats from 15th to 18th day of pregnancy using tyloxapol (300 mg / kg daily). As drugs for monotherapy, Essentiale (Sanofi-Aventis, France) and Heptral (Abbott srl, Italy) were used separately and were administered to rats daily from the 16th to the 18th day of pregnancy (50 mg / kg and 35 mg / kg, respectively). On the 19th day of pregnancy, blood serum biochemical parameters were analyzed.
RESULTS: In the model, the concentrations of triglycerides and cholesterol were sharply increased, which can be caused not only by the inhibitory effect of tyloxapol on the lipoprotein lipase of the lumen of blood vessels, but also by damage to liver cells with the accumulation of lipids in them. The activities of aminotransferases (ALT, AST), glutamate dehydrogenase and lactate dehydrogenase were statistically significantly increased. In healthy pregnant rats, the activity of alkaline phosphatase (ALP) decreased from 15th to 19th day of gestation, but under the treatment by tyloxapol in some cases the increase of ALP activity was observed. After using essential phospholipids in the model of acute liver injury, normal values of ALT and ALP activities were observed, and a significant decrease in triglycerides was revealed, which did not reach the normal values. After using S-adenosyl methionine in this model, the significant changes in blood serum parameters were not observed. By themselves, the drugs change several biochemical parameters in the serum of healthy pregnant rats. Heptral increases urea levels and Essentiale appears to increase AST activity.
CONCLUSION: The use of essential phospholipids or S-adenosyl methionine as monotherapy in a model of acute liver damage in late pregnancy has demonstrated their low efficiency. It is possible that the combined use of drugs is necessary to increase the effectiveness. The use of S-adenosyl methionine should be carried out under the control of the urea level and glutamate dehydrogenase activity in serum. While using essential phospholipids, it is advisable to control the AST activity.
Full Text
About the authors
Mariya A. Petrosyan
D.O. Ott Institute of Obstetrics, Gynecology and Reproductolog; Almazov National Medical Research Centre
Email: marya@labpharm.spb.ru
ORCID iD: 0000-0001-7347-6104
SPIN-code: 5329-5420
PhD, Cand. Sci. (Biol.)
Russian Federation, 3 Mendeleevskaya line, Saint Petersburg, 199034; Saint PetersburgNatalia V. Zhestkova
D.O. Ott Institute of Obstetrics, Gynecology and Reproductolog
Email: zhestkova@me.com
ORCID iD: 0000-0001-8078-3524
SPIN-code: 6014-8153
PhD, Dr. Sci. (Med.)
Russian Federation, 3 Mendeleevskaya line, Saint Petersburg, 199034Zhanna N. Tumasova
D.O. Ott Institute of Obstetrics, Gynecology and Reproductolog
Email: j_toumassova@mail.ru
ORCID iD: 0000-0001-8585-2250
doctor of laboratory diagnostics
Russian Federation, 3 Mendeleevskaya line, Saint Petersburg, 199034Lyudmila S. Polyanskikh
D.O. Ott Institute of Obstetrics, Gynecology and Reproductolog
Email: polyanskikh-83@mail.ru
ORCID iD: 0000-0001-9994-8341
SPIN-code: 2501-8880
scientific researcher
Russian Federation, 3 Mendeleevskaya line, Saint Petersburg, 199034Natalia N. Balashova
D.O. Ott Institute of Obstetrics, Gynecology and Reproductolog
Email: nb-zok@bk.ru
ORCID iD: 0000-0002-2655-9481
SPIN-code: 1973-9953
junior researcher
Russian Federation, 3 Mendeleevskaya line, Saint Petersburg, 199034Elena V. Baziian
D.O. Ott Institute of Obstetrics, Gynecology and Reproductolog
Email: waz2107gen@yandex.ru
ORCID iD: 0000-0001-7837-3315
SPIN-code: 2232-9914
scientific researcher
Russian Federation, 3 Mendeleevskaya line, Saint Petersburg, 199034Tatiana U. Kuz’minykh
Saint Petersburg State University
Email: 9260@mail.ru
ORCID iD: 0000-0002-6136-5324
Dr. Sci. (Med.)
Russian Federation, Saint PetersburgAlexey V. Razygraev
D.O. Ott Institute of Obstetrics, Gynecology and Reproductolog; St. Petersburg State Chemical-Pharmaceutical University; Zoological Institute Russian Academy of Sciences
Author for correspondence.
Email: a.v.razygraev@gmail.com
ORCID iD: 0000-0002-0544-9398
SPIN-code: 8623-7923
PhD, Cand. Sci. (Med.)
Russian Federation, 3 Mendeleevskaya line, Saint Petersburg, 199034; Saint Petersburg; Saint PetersburgReferences
- Kulikov AV, Shifman EM, Spirin AV. The hepatic pathology in obstetrics. Russian Medical Journal. 2014;20(2):37–44. (In Russ.)
- Millar JS, Cromley DA, McCoy MG, et al. Determining hepatic triglyceride production in mice: comparison of poloxamer 407 with Triton WR-1339. J Lipid Res. 2005;46(9):2023–2028. doi: 10.1194/jlr.D500019-JLR200
- Ayo-Lawal AR, Osoniyi RO. Antihyperlipidaemic and Antioxidant Potential of Fermented Citrullus vulgaris Seeds (Thunb.) on Tyloxapol-induced Hyperlipidaemic Rats: A Comparison with Fluvastatin. Journal of Food Research. 2015;4(4):110–119. doi: 10.5539/jfr.v4n4p110
- Shen B, Zhao C, Wang Y, et al. Aucubin inhibited lipid accumulation and oxidative stress via Nrf2/HO-1 and AMPK signalling pathways. J Cell Mol Med. 2019;23(6):4063–4075. doi: 10.1111/jcmm.14293
- Patent RU2610536 / 13.02.2017. Byul. No. 8. Petrosyan MA, Zhestkova NV, Balashova NN, et al. Method for simulating acute liver disease in pregnant women. (In Russ.) Available from: https://patenton.ru/patent/RU2610536C1. Cited: 2021 Feb 24.
- Zil’fyan VN, Kumkumadzhyan VA. Novyj metod vzjatija krovi u melkih laboratornyh zhivotnyh. Zhurnal Eksperimental’noi i klinicheskoi meditsiny. 1970;10(4):12–14. (In Russ.)
- R Core Team. R: A Language and Environment for Statistical Computing. R Foundation for Statistical Computing. Vienna; 2017. Available from: https://www.R-project.org/
- Jaeschke H, McGill MR. Serum glutamate dehydrogenase — biomarker for liver cell death or mitochondrial dysfunction? Toxicol Sci. 2013;134(1):221–222. doi: 10.1093/toxsci/kft087
- Beigneux AP, Davies BS, Gin P, et al. Glycosylphosphatidylinositol-anchored high-density lipoprotein-binding protein 1 plays a critical role in the lipolytic processing of chylomicrons. Cell Metab. 2007;5(4):279–291. doi: 10.1016/j.cmet.2007.02.002
- Davies BS, Beigneux AP, Barnes RH, et al. GPIHBP1 is responsible for the entry of lipoprotein lipase into capillaries. Cell Metab. 2010;12(1):42–52. doi: 10.1016/j.cmet.2010.04.016
- Kamyshnikov VS. Clinical and laboratory diagnosis of liver diseases. Laboratory diagnostics. Eastern Europe. 2016;5(1):150–163. (In Russ.)
- Westbrook RH, Dusheiko G, Williamson C. Pregnancy and liver disease. J Hepatol. 2016;64(4):933–945. doi: 10.1016/j.jhep.2015.11.030