Using zebrafish (Danio rerio) to assess short-term memory: the habituation and the homebase tests

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Abstract

Environmental novelty is one of the most potent stressors in animals and is often used in behavioral neuroscience to study affective and cognitive impairments. However, in the process of studying an unfamiliar environment in experimental animals, there is a decrease in stress due to habituation (adaptation, habituation). In various behavioral tests in zebrafish, this manifests as swimming in areas that pose a potential danger to them: the upper part of the aquarium in the novel tank test and the central part in the open field test. When building an effective survival strategy, it is important to navigate in an unfamiliar environment from a home base — the safest area that serves as a starting point in exploring a novel arena. Both discussed here, habitation and establishing the home base, are important for assessing cognitive behavioral traits in zebrafish related to short-term spatial working memory.

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About the authors

David S. Galstyan

Saint Petersburg State University; A.M. Granov Russian Research Center for Radiology and Surgical Technologies; Almazov National Medical Research Centre

Email: david_sam@mail.ru
ORCID iD: 0000-0002-6213-5117

Research Associate

Russian Federation, Saint Petersburg; Saint Petersburg; Saint Petersburg

Tatyana O. Kolesnikova

Sirius University of Science and Technology

Email: philimontani@yandex.ru
ORCID iD: 0000-0002-5561-8583
SPIN-code: 8558-7887

Research Associate

Russian Federation, Sochi

Yurii M. Kositsyn

Saint Petersburg State University

Email: ikosicin53@gmail.com
ORCID iD: 0000-0002-4266-808X

Research Associate

Russian Federation, Saint Petersburg

Konstantin N. Zabegalov

Sirius University of Science and Technology

Email: hatokiri@mail.ru
ORCID iD: 0000-0002-9748-0324
SPIN-code: 5993-6315

Research Associate

Russian Federation, Sochi

Mariya A. Gubaidullina

Sirius University of Science and Technology

Email: mariangub@gmail.com

Research Associate

Russian Federation, Sochi

Gleb O. Maslov

Sirius University of Science and Technology; Ural Federal University

Email: maslovog6@gmail.com

Research Associate

Russian Federation, Sochi; Yekaterinburg

Konstantin A. Demin

Saint Petersburg State University; Sirius University of Science and Technology; Almazov National Medical Research Centre

Email: deminkasci@gmail.com
SPIN-code: 3830-1853

Cand. Sci. (Biol.), Senior Research Associate

Russian Federation, Saint Petersburg; Sochi; Saint Petersburg

Allan V. Kalueff

Saint Petersburg State University; A.M. Granov Russian Research Center for Radiology and Surgical Technologies; Sirius University of Science and Technology; Almazov National Medical Research Centre; Ural Federal University; Novosibirsk State University; Research Institute of Neuroscience and Medicine; Moscow Institute of Physics and Technology

Author for correspondence.
Email: avkalueff@gmail.com
ORCID iD: 0000-0002-7525-1950
SPIN-code: 4134-0515

Dr. Sci. (Biol.), Professor

Russian Federation, Saint Petersburg; Saint Petersburg; Sochi; Saint Petersburg; Yekaterinburg; Novosibirsk; Novosibirsk; Moscow

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Copyright (c) 2022 Galstyan D.S., Kolesnikova T.O., Kositsyn Y.M., Zabegalov K.N., Gubaidullina M.A., Maslov G.O., Demin K.A., Kalueff A.V.

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