Using zebrafish (Danio rerio) to assess short-term memory: the habituation and the homebase tests
- Authors: Galstyan D.S.1,2,3, Kolesnikova T.O.4, Kositsyn Y.M.1, Zabegalov K.N.4, Gubaidullina M.A.4, Maslov G.O.4,5, Demin K.A.1,4,3, Kalueff A.V.1,2,4,3,5,6,7,8
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Affiliations:
- Saint Petersburg State University
- A.M. Granov Russian Research Center for Radiology and Surgical Technologies
- Almazov National Medical Research Centre
- Sirius University of Science and Technology
- Ural Federal University
- Novosibirsk State University
- Research Institute of Neuroscience and Medicine
- Moscow Institute of Physics and Technology
- Issue: Vol 20, No 2 (2022)
- Pages: 169-175
- Section: Original articles
- Submitted: 09.08.2022
- Accepted: 12.08.2022
- Published: 03.10.2022
- URL: https://journals.eco-vector.com/RCF/article/view/109706
- DOI: https://doi.org/10.17816/RCF202169-175
- ID: 109706
Cite item
Abstract
Environmental novelty is one of the most potent stressors in animals and is often used in behavioral neuroscience to study affective and cognitive impairments. However, in the process of studying an unfamiliar environment in experimental animals, there is a decrease in stress due to habituation (adaptation, habituation). In various behavioral tests in zebrafish, this manifests as swimming in areas that pose a potential danger to them: the upper part of the aquarium in the novel tank test and the central part in the open field test. When building an effective survival strategy, it is important to navigate in an unfamiliar environment from a home base — the safest area that serves as a starting point in exploring a novel arena. Both discussed here, habitation and establishing the home base, are important for assessing cognitive behavioral traits in zebrafish related to short-term spatial working memory.
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About the authors
David S. Galstyan
Saint Petersburg State University; A.M. Granov Russian Research Center for Radiology and Surgical Technologies; Almazov National Medical Research Centre
Email: david_sam@mail.ru
ORCID iD: 0000-0002-6213-5117
Research Associate
Russian Federation, Saint Petersburg; Saint Petersburg; Saint PetersburgTatyana O. Kolesnikova
Sirius University of Science and Technology
Email: philimontani@yandex.ru
ORCID iD: 0000-0002-5561-8583
SPIN-code: 8558-7887
Research Associate
Russian Federation, SochiYurii M. Kositsyn
Saint Petersburg State University
Email: ikosicin53@gmail.com
ORCID iD: 0000-0002-4266-808X
Research Associate
Russian Federation, Saint PetersburgKonstantin N. Zabegalov
Sirius University of Science and Technology
Email: hatokiri@mail.ru
ORCID iD: 0000-0002-9748-0324
SPIN-code: 5993-6315
Research Associate
Russian Federation, SochiMariya A. Gubaidullina
Sirius University of Science and Technology
Email: mariangub@gmail.com
Research Associate
Russian Federation, SochiGleb O. Maslov
Sirius University of Science and Technology; Ural Federal University
Email: maslovog6@gmail.com
Research Associate
Russian Federation, Sochi; YekaterinburgKonstantin A. Demin
Saint Petersburg State University; Sirius University of Science and Technology; Almazov National Medical Research Centre
Email: deminkasci@gmail.com
SPIN-code: 3830-1853
Cand. Sci. (Biol.), Senior Research Associate
Russian Federation, Saint Petersburg; Sochi; Saint PetersburgAllan V. Kalueff
Saint Petersburg State University; A.M. Granov Russian Research Center for Radiology and Surgical Technologies; Sirius University of Science and Technology; Almazov National Medical Research Centre; Ural Federal University; Novosibirsk State University; Research Institute of Neuroscience and Medicine; Moscow Institute of Physics and Technology
Author for correspondence.
Email: avkalueff@gmail.com
ORCID iD: 0000-0002-7525-1950
SPIN-code: 4134-0515
Dr. Sci. (Biol.), Professor
Russian Federation, Saint Petersburg; Saint Petersburg; Sochi; Saint Petersburg; Yekaterinburg; Novosibirsk; Novosibirsk; MoscowReferences
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