Molecular screening of squash and patisson squash collection samples using markers of the Pm-0 gene, which controls resistance to powdery mildew

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Abstract

Powdery mildew (PM) is one of the most harmful diseases of cucurbits. Modern commercial varieties of squash Cucurbita pepo L. var. giraumonas Duch and patisson C. pepo var. melopepo L. received powdery mildew resistance genes from wild species. The Pm-0 resistance gene belongs to the linkage group 10; two CAPS markers were developed for its mapping [15]. The main intragenic marker NBS_S9_1495924/HaeIII is localized in the NBS-LRR region, the additional marker S9_1539675/MspI shows complete co-segregation with resistance to PM [15]. In the present study, these markers were used for molecular screening of an experimental set of squash and patisson samples (differ in resistance to powdery mildew) from the VIR gene bank collection and perspective breeding lines of the Krymsk Experiment Breeding Station – branch of VIR. In total, 80 samples were investigated. Samples, carrying fragments of both CAPS markers (17) and fragments of only one marker (31) were found. Presence of two markers of the Pm-0 gene (NBS_S9_1495924/HaeIII и S9_1539675/MspI) has the strong correlation with resistance to PM (r = 0.837).

For three powdery mildew-resistant samples, which has demonstrated presence of both markers of the Pm-0 gene, additional analysis of individual plants was done. Resistant plants with Pm-0 gene markers were self-pollinated to create resistant lines. Thus, molecular screening allowed to preserve the valuable trait of resistance during maintaining of the squash collection samples.

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About the authors

Fedor A. Berensen

N.I. Vavilov All-Russian Institute of Plant Genetic Resources

Author for correspondence.
Email: fberensen@gmail.com
ORCID iD: 0000-0002-0492-2024
SPIN-code: 1497-7502

research associate

Russian Federation, Saint Petersburg

Tatiana M. Piskunova

N.I. Vavilov All-Russian Institute of Plant Genetic Resources

Email: tmpiskunova@yandex.ru
ORCID iD: 0000-0002-9267-6619
SPIN-code: 6122-0333

Cand. Sci. (Agriculture), leading research associate

Russian Federation, Saint Petersburg

Semyon V. Kuzmin

Krymsk Experiment Breeding Station — N.I. Vavilov All-Russian Institute of Plant Genetic Resources

Email: sem290@mail.ru
SPIN-code: 9137-2253

Cand. Sci. (Agriculture), senior research associate

Russian Federation, Krymsk

Andrey F. Moskalu

N.I. Vavilov All-Russian Institute of Plant Genetic Resources

Email: andromos2015@yandex.ru

junior research associate

Russian Federation, Saint Petersburg

Olga Y. Antonova

N.I. Vavilov All-Russian Institute of Plant Genetic Resources

Email: olgaant326@mail.ru
ORCID iD: 0000-0001-8334-8069
SPIN-code: 9255-6449
Scopus Author ID: 23391684100

Cand. Sci. (Biol.)

Russian Federation, Saint Petersburg

Anna M. Artemyeva

N.I. Vavilov All-Russian Institute of Plant Genetic Resources

Email: akme11@yandex.ru
ORCID iD: 0000-0002-6551-5203
SPIN-code: 8776-7367
Scopus Author ID: 14014607500

Cand. Sci. (Agriculture), leading research associate

Russian Federation, Saint Petersburg

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2. Fig. 1. Molecular screening of squash and patisson squash samples (3–4 individual plants per sample) for Pm-0 resistance gene markers: А — marker NBS_S9_1495924; Б — marker S9_1539675. The arrows indicate diagnostic fragments associated with resistance. 1 — negative control (water); 2–4 — Lena F1; 5–8 — Nevira F1; 9–12 — Ardendo 174 F1; 13–16 — Gribovsky 37; 17–20 — Sukha F1; 21–24 — Casanova F1; 25–28 — Aral F1; 29–32 — line Ds 4; M — molecular weight marker “100+ bp DNA Ladder”, Eurogen (Russia)

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