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Assembly of genetic constructs for analysis of three promoters in plants
- Authors: Gancheva M.S.1, Safronova D.D.2, Valitova E.V.2, Dulesov M.A.2, Chebykina A.E.2, Solovyov Y.E.2, Semenova T.V.2, Mamonova K.M.2, Volchkov A.M.2, Fomina D.M.2, Korneva S.S.2, Lutova L.A.1
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Affiliations:
- Saint Petersburg State University
- Sirius University
- Issue: Vol 20 (2022): Supplement
- Pages: 22-23
- Section: Genetically modified organism. The Нistory, Achivements, Social and Environmental Riscs
- URL: https://journals.eco-vector.com/ecolgenet/article/view/112335
- DOI: https://doi.org/10.17816/ecogen112335
- ID: 112335
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Abstract
To study the processes of plant development, it is often necessary to find out in which tissues and cells the gene is expressed. To do this, genetic constructs with promoters and fluorescence reporters are used.
The aim of our work is to create a construct for the simultaneous analysis of the activity of three promoters: auxin-responsive DR5 promoter, cytokinin sensor TCS, and any other promoter necessary for research purposes. We have selected fluorescent proteins with different spectral characteristics that make it easy to distinguish them from each other: red (mCherry), blue (TagBFP), green (mNeonGreen). Hygromycin B resistance gene we use to select transgenic plants. For cloning, we used the MoClo vector system. So far, we have created 5 plasmids containing hygromycin resistance gene, mCherry, mTagBFP, and DR5 and TCS promoters. The next step is to create a single vector that will contain all components.
This work was supported by the Ministry of Science and Higher Education of the Russian Federation in accordance with agreement No. 075-15-2022-322 date 22.04.2022 on providing a grant in the form of subsidies from the Federal budget of Russian Federation. The grant was provided for state support for the creation and development of a World-class Scientific Center “Agrotechnologies for the Future”.
Full Text
To study the processes of plant development, it is often necessary to find out in which tissues and cells the gene is expressed. To do this, genetic constructs with promoters and fluorescence reporters are used.
The aim of our work is to create a construct for the simultaneous analysis of the activity of three promoters: auxin-responsive DR5 promoter, cytokinin sensor TCS, and any other promoter necessary for research purposes. We have selected fluorescent proteins with different spectral characteristics that make it easy to distinguish them from each other: red (mCherry), blue (TagBFP), green (mNeonGreen). Hygromycin B resistance gene we use to select transgenic plants. For cloning, we used the MoClo vector system. So far, we have created 5 plasmids containing hygromycin resistance gene, mCherry, mTagBFP, and DR5 and TCS promoters. The next step is to create a single vector that will contain all components.
This work was supported by the Ministry of Science and Higher Education of the Russian Federation in accordance with agreement No. 075-15-2022-322 date 22.04.2022 on providing a grant in the form of subsidies from the Federal budget of Russian Federation. The grant was provided for state support for the creation and development of a World-class Scientific Center “Agrotechnologies for the Future”.
About the authors
Maria S. Gancheva
Saint Petersburg State University
Email: ganchovai@gmail.com
ORCID iD: 0000-0002-9631-6143
Scopus Author ID: 56336247600
PhD
Russian Federation, Saint PetersburgDaria D. Safronova
Sirius University
Email: vaiagan@mail.ru
Student
Russian Federation, SochiEkaterina V. Valitova
Sirius University
Email: vaiagan@mail.ru
Student
Russian Federation, SochiMichail A. Dulesov
Sirius University
Email: vaiagan@mail.ru
Student
Russian Federation, SochiArina E. Chebykina
Sirius University
Email: vaiagan@mail.ru
Student
Russian Federation, SochiYaroslav E. Solovyov
Sirius University
Email: vaiagan@mail.ru
Student
Russian Federation, SochiTatiana V. Semenova
Sirius University
Email: vaiagan@mail.ru
Student
Russian Federation, SochiKseniya M. Mamonova
Sirius University
Email: vaiagan@mail.ru
Student
Russian Federation, SochiAndrey M. Volchkov
Sirius University
Email: vaiagan@mail.ru
Student
Russian Federation, SochiDarina M. Fomina
Sirius University
Email: vaiagan@mail.ru
Student
Russian Federation, SochiSofia S. Korneva
Sirius University
Email: vaiagan@mail.ru
Student
Russian Federation, SochiLudmila A. Lutova
Saint Petersburg State University
Author for correspondence.
Email: la.lutova@gmail.com
Doctor of Science, Professor, Department of Genetics and Biotechnology
Russian Federation, Saint PetersburgReferences
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