Komagataella phaffii yeast as a model organism in biotechnology and fundamental research
- Authors: Rumyantsev A.M.1, Sidorin A.V.1, Ianshina T.M.1, Petrova K.D.1, Ishtuganova V.V.1, Sambuk E.V.1, Padkina M.V.1
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Affiliations:
- Saint Petersburg State University
- Issue: Vol 20 (2022): Supplement
- Pages: 13-14
- Section: Genetically modified organism. The Нistory, Achivements, Social and Environmental Riscs
- Submitted: 03.11.2022
- Accepted: 09.11.2022
- Published: 08.12.2022
- URL: https://journals.eco-vector.com/ecolgenet/article/view/112353
- DOI: https://doi.org/10.17816/ecogen112353
- ID: 112353
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Abstract
The choice of a model organism in biology is based on various factors, such as practical significance and ease of manipulation. Saccharomyces cerevisiae yeast is one of the most widely used and well-studied eukaryotic models. However, developments in NGS, proteomics, metabolomics and gene editing methods allow other species to become the object of fundamental research. A good example of such emerging model organisms is the yeast Komagataella phaffii.
K. phaffii belongs to a unique group of eukaryotic methylotrophs that can use methanol as the sole source of carbon and energy. On the other hand, K. phaffii seems to be more characteristic of the common ancient yeast ancestors than the rapidly evolving S. cerevisiae. Comparative studies between S. cerevisiae and K. phaffii will shed light on the mechanisms of evolution of metabolic pathways and regulatory systems. Such studies are accelerated by the practical importance of K. phaffii as a common microbial production host in biotechnology.
In our studies, we demonstrate that some amino acids greatly affect gene expression in K. phaffii [1]. Transcriptome analysis revealed drastic changes in gene expression when proline was present in the media. About 18.9% of total protein-coding genes were differentially expressed, including genes involved in methanol utilization [2]. Our results show that the unique methanol metabolism pathway is regulated not only by methanol, but also by other carbon sources. Therefore, our findings suggest that the regulation of methanol metabolism pathway is integrated into other cellular regulatory networks. Methanol metabolism, acquired by K. phaffii during evolution, is tightly associated with nitrogen an amino acid metabolism.
Full Text
The choice of a model organism in biology is based on various factors, such as practical significance and ease of manipulation. Saccharomyces cerevisiae yeast is one of the most widely used and well-studied eukaryotic models. However, developments in NGS, proteomics, metabolomics and gene editing methods allow other species to become the object of fundamental research. A good example of such emerging model organisms is the yeast Komagataella phaffii.
K. phaffii belongs to a unique group of eukaryotic methylotrophs that can use methanol as the sole source of carbon and energy. On the other hand, K. phaffii seems to be more characteristic of the common ancient yeast ancestors than the rapidly evolving S. cerevisiae. Comparative studies between S. cerevisiae and K. phaffii will shed light on the mechanisms of evolution of metabolic pathways and regulatory systems. Such studies are accelerated by the practical importance of K. phaffii as a common microbial production host in biotechnology.
In our studies, we demonstrate that some amino acids greatly affect gene expression in K. phaffii [1]. Transcriptome analysis revealed drastic changes in gene expression when proline was present in the media. About 18.9% of total protein-coding genes were differentially expressed, including genes involved in methanol utilization [2]. Our results show that the unique methanol metabolism pathway is regulated not only by methanol, but also by other carbon sources. Therefore, our findings suggest that the regulation of methanol metabolism pathway is integrated into other cellular regulatory networks. Methanol metabolism, acquired by K. phaffii during evolution, is tightly associated with nitrogen an amino acid metabolism.
About the authors
Andrey M. Rumyantsev
Saint Petersburg State University
Email: rumyantsev-am@mai.ru
SPIN-code: 9335-1184
Anton V. Sidorin
Saint Petersburg State University
Email: spacerocketpilot@gmail.com
Master of Science, PhD Student, Department of Genetics and Biotechnology
Russian Federation, Saint PetersburgTatiana M. Ianshina
Saint Petersburg State University
Email: t.ianshina99@gmail.com
Bachelor, Student, Department of Genetics and Biotechnology
Russian Federation, Saint PetersburgKristina D. Petrova
Saint Petersburg State University
Email: seeu.chan.voca@gmail.com
Student, Department of Genetics and Biotechnology
Russian Federation, Saint PetersburgValeriia V. Ishtuganova
Saint Petersburg State University
Email: st086860@student.spbu.ru
Student, Department of Genetics and Biotechnology
Russian Federation, Saint PetersburgElena V. Sambuk
Saint Petersburg State University
Email: esambuk@mail.ru
SPIN-code: 8281-8020
Doctor of Science, Professor of the Department of Genetics and Biotechnology
Russian Federation, Saint PetersburgMarina V. Padkina
Saint Petersburg State University
Author for correspondence.
Email: mpadkina@mail.ru
SPIN-code: 7709-0449
Doctor of Science, Professor of the Department of Genetics and Biotechnology
Russian Federation, Saint PetersburgReferences
- Rumjantsev AM, Bondareva OV, Padkina MV, Sambuk EV. Effect of nitrogen source and inorganic phosphate concentration on methanol utilization and PEX genes expression in Pichia pastoris. Sci World J. 2014;2014:743615. doi: 10.1155/2014/743615
- Rumyantsev A, Sidorin A, Volkov A, et al. Transcriptome Analysis Unveils the Effects of Proline on Gene Expression in the Yeast Komagataella phaffii. Microorganisms. 2021;10(1):67. doi: 10.3390/microorganisms10010067