Evolution and epidemiology of global populations of nursery-associated Agrobacterium
- Авторы: Weisberg A.1, Davis II E.1, Tabima J.1, Putnam M.1, Miller M.1, Belcher M.1, Grünwald N.1,2, Ream W.3, Lai E.3, Kuo C.3, Loper J.1,2, Chang J.1
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Учреждения:
- Oregon State University
- Agricultural Research Service
- Academia Sinica
- Выпуск: Том 20 (2022): Спецвыпуск
- Страницы: 26-27
- Раздел: «ГМО: ИСТОРИЯ, ДОСТИЖЕНИЯ, СОЦИАЛЬНЫЕ И ЭКОЛОГИЧЕСКИЕ РИСКИ»
- Статья получена: 04.11.2022
- Статья одобрена: 04.11.2022
- Статья опубликована: 08.12.2022
- URL: https://journals.eco-vector.com/ecolgenet/article/view/112374
- DOI: https://doi.org/10.17816/ecogen112374
- ID: 112374
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Аннотация
Agrobacterium tumefaciens is unique in that it can facilitate the interkingdom transfer of DNA and genetically modify its plant host. While Agrobacterium has been coopted for use in the genetic modification of plants, it is also a major pathogen, causing crown gall disease in the nursery, orchard, and vineyard industries. Pathogenicity in Agrobacterium is the result of two components. First is the Ti plasmid, which carries virulence genes and the transferred T-DNA region. The second component is the chromosome of Agrobacterium, which comprises diverse bacterial lineages and multiple species-level groups. The Ti plasmid can be transferred from strain to strain, diversifying the pathogen and complicating efforts to understand its epidemiology. This system provides an opportunity to study transmission of plasmids and their impact on disease persistence and spread. However, the movement of plasmids, and diversity of chromosomal lineages, means that conventional methods of using whole genome SNPs to track outbreaks are not sufficient, and new techniques must be developed. Additionally, Ti plasmids, like Agrobacterium, are genetically diverse and represent multiple plasmid types. Using a framework of >200 sequenced Agrobacterium genomes isolated from around the world, and a previously developed model of Ti plasmid types, we modelled their epidemiology. Key to this study was that we first separately analyzed plasmids and strain. Combining results revealed links between nurseries, potential horizontal transfer of the plasmid between strains within nurseries, global spread of plasmids, and long-term persistence of plasmids in the agricultural system. Agricultural practices have the potential to promote the diversification of pathogens and the emergence of new pathogen lineages.
Полный текст
Agrobacterium tumefaciens is unique in that it can facilitate the interkingdom transfer of DNA and genetically modify its plant host. While Agrobacterium has been coopted for use in the genetic modification of plants, it is also a major pathogen, causing crown gall disease in the nursery, orchard, and vineyard industries. Pathogenicity in Agrobacterium is the result of two components. First is the Ti plasmid, which carries virulence genes and the transferred T-DNA region. The second component is the chromosome of Agrobacterium, which comprises diverse bacterial lineages and multiple species-level groups. The Ti plasmid can be transferred from strain to strain, diversifying the pathogen and complicating efforts to understand its epidemiology. This system provides an opportunity to study transmission of plasmids and their impact on disease persistence and spread. However, the movement of plasmids, and diversity of chromosomal lineages, means that conventional methods of using whole genome SNPs to track outbreaks are not sufficient, and new techniques must be developed. Additionally, Ti plasmids, like Agrobacterium, are genetically diverse and represent multiple plasmid types. Using a framework of >200 sequenced Agrobacterium genomes isolated from around the world, and a previously developed model of Ti plasmid types, we modelled their epidemiology. Key to this study was that we first separately analyzed plasmids and strain. Combining results revealed links between nurseries, potential horizontal transfer of the plasmid between strains within nurseries, global spread of plasmids, and long-term persistence of plasmids in the agricultural system. Agricultural practices have the potential to promote the diversification of pathogens and the emergence of new pathogen lineages.
Об авторах
Alexandra Weisberg
Oregon State University
Email: weisbeal@oregonstate.edu
Scopus Author ID: 5636735170
Department of Botany and Plant Pathology
США, Corvallis, OR, 97331Edward Davis II
Oregon State University
Email: davised@oregonstate.edu
Scopus Author ID: 55328495700
Department of Botany and Plant Pathology
США, Corvallis, OR, 97331Javier Tabima
Oregon State University
Email: tabimaj@oregonstate.edu
Scopus Author ID: 37262084000
Department of Botany and Plant Pathology
США, Corvallis, OR, 97331Melodie Putnam
Oregon State University
Email: melodie.putnam@oregonstate.edu
Scopus Author ID: 7005638450
Department of Botany and Plant Pathology
США, Corvallis, OR, 97331Marilyn Miller
Oregon State University
Email: millmari28@gmail.com
Scopus Author ID: 57223801124
Department of Botany and Plant Pathology
США, Corvallis, OR, 97331Michael Belcher
Oregon State University
Email: belcher_michael@berkeley.edu
Scopus Author ID: 57200333901
Department of Botany and Plant Pathology
США, Corvallis, OR, 97331Niklaus Grünwald
Oregon State University; Agricultural Research Service
Email: niklaus.grunwald@oregonstate.edu
Scopus Author ID: 57581518400
Department of Botany and Plant Pathology, Horticultural Crops Research Laboratory, US Department of Agriculture
США, Corvallis, OR, 97331; Corvallis, OR 97331Walt Ream
Academia Sinica
Email: reamw@science.oregonstate.edu
Scopus Author ID: 6603918074
Department of Microbiology
Китайская республика, Taiwan, Taipei 11529Erh-Min Lai
Academia Sinica
Email: emlai@gate.sinica.edu.tw
Scopus Author ID: 36110147800
Institute of Plant and Microbial Biology
Китайская республика, Taiwan, Taipei 11529Chih-Horng Kuo
Academia Sinica
Email: chk@gate.sinica.edu.tw
Scopus Author ID: 35076199500
Institute of Plant and Microbial Biology
Китайская республика, Taiwan, Taipei 11529Joyce Loper
Oregon State University; Agricultural Research Service
Email: joyce.loper@oregonstate.edu
Scopus Author ID: 7006458001
Department of Botany and Plant Pathology, Horticultural Crops Research Laboratory, US Department of Agriculture
США, Corvallis, OR, 97331; Corvallis, OR 97331Jeff Chang
Oregon State University
Автор, ответственный за переписку.
Email: jeff.chang@oregonstate.edu
Scopus Author ID: 7601552801
Department of Botany and Plant Pathology
США, Corvallis, OR, 97331Список литературы
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