Genetic differentiation of two phenotypes of Plantago media L. In South Timan

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Abstract


Background. The investigation of the genetic nature of plant phenotypic variability is of great importance for understanding biological diversity, distribution and adaptation of plants to environmental conditions.

The aim of our work was to study the genetic differentiation of two phenotypes of Plantago media in South Timan.

Materials and methods. The genetic differentiation of light and shadow phenotypes of Plantago media plants was evaluated using intersimple sequence repeats (ISSR) markers.

Results. The population-genetic analysis of 210 loci revealed two clusters, which boundaries coincided with the boundaries between plants of light and shadow phenotypes. The results of the discriminatory analysis of the main components and AMOVA (FST = 0.07, p = 0.001) confirmed that there are statistically significant genetic differences between these phenotypes even though they possess a high genetic similarity.

Conclusion. Light and shadow Plantago media phenotypes adapted to different ecological conditions are genetically differentiated. The population genetic analysis using ISSR markers is a sensitive tool for identifying the genetic diversity of phenotypic plant variations formed under the influence of environmental factors.


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About the authors

Ilya G. Zakhozhiy

Institute of Biology of Komi Science Centre of the Ural Branch of the Russian Academy of Sciences

Author for correspondence.
Email: zakhozhiy@ib.komisc.ru
ORCID iD: 0000-0003-0918-745X
SPIN-code: 3615-9301
Scopus Author ID: 36613922200
ResearcherId: P-9869-2015
Mendeley Profile: ilya-zakhozhiy

Russian Federation, Syktyvkar

PhD, Researcher, Laboratory of Ecological Physiology of Plants

Dmitry M. Shadrin

Institute of Biology of Komi Science Centre of the Ural Branch of the Russian Academy of Sciences

Email: shdimas@yandex.ru
ORCID iD: 0000-0003-4365-0145
SPIN-code: 1453-3893
Scopus Author ID: 56644695300
ResearcherId: P-9808-2015

Russian Federation, Syktyvkar

PhD, Researcher, Center for Collective Use “Molecular Biology”

Yana I. Pylina

Institute of Biology of Komi Science Centre of the Ural Branch of the Russian Academy of Sciences

Email: yanapylina@yandex.ru
ORCID iD: 0000-0003-4981-8930
SPIN-code: 5636-5693
Scopus Author ID: 56644812300
ResearcherId: P-9565-2015

Russian Federation, Syktyvkar

Junior Research Scientist, Center for Collective Use “Molecular Biology”

Ivan F. Chadin

Institute of Biology of Komi Science Centre of the Ural Branch of the Russian Academy of Sciences

Email: chadin@ib.komisc.ru
ORCID iD: 0000-0001-6299-2285
SPIN-code: 9787-2449
Scopus Author ID: 7801456952
ResearcherId: P-4940-2015

Russian Federation, Syktyvkar

PhD, Head of Moleculal Biology Core Facility

Tamara K. Golovko

Institute of Biology of Komi Science Centre of the Ural Branch of the Russian Academy of Sciences

Email: golovko@ib.komisc.ru
ORCID iD: 0000-0002-7993-9541
SPIN-code: 4344-7144
Scopus Author ID: 7004365574
ResearcherId: P-4922-2015

Russian Federation, Syktyvkar

Doctor of Science, Main Researcher, Laboratory of Ecological Physiology of Plants

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Supplementary files

Supplementary Files Action
1.
Fig. 1. Daily changes in illumination (a), ultraviolet radiation intensity (b) and air temperature (c) in Plantago media habitats: 1 - on an open slope, 2 - in the forest. The measurements were performed in the first decade of July 2014 on a clear sunny day. PAR - photosynthetically active radiation

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2.
Fig. 2. Clustering results of plant samples of P. media and P. major at 210 ISSR loci in the STRUCTURE program [13]. Horizontal: each individual column corresponds to one sample; 1 and 2 - shadow and light plants P. media; 3 - plants of P. major. Vertical - the probability of assigning each sample (individual) to one of the clusters. Variants of the number of clusters are indicated above the graphs: K = 2 - the most probable number of clusters, determined by the Evanno method [15]; K = 3 - the number of clusters corresponding to the number of studied plant groups

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3.
Fig. 3. Scatter plot based on the discriminatory analysis of principal components (DAPC) of a binary matrix containing 210 ISSR loci of plants P. media and P. major using 5 major components. 1 and 2 - P. media plants from the forest ecotope and on the open slope, respectively; 3 - P. major plants. Axis 1 - values of the discriminatory function 1, axis 2 - values of the discriminatory function 2. The relative values of the eigenvalues DAPC are presented in the lower right corner of the graph

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Copyright (c) 2020 Zakhozhiy I.G., Shadrin D.M., Pylina Y.I., Chadin I.F., Golovko T.K.

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