Analysis of introgressive lines of inter-species pea hybrids by band composition of seed proteins

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Abstract


Background. The reproductive incompatibility of cultivated (Pisum sativum) and wild (P. fulvum) pea species determines the difficulties of obtaining hybrids as well as the transfer of valuable wild parent alleles into interspecific hybrids and their use in the breeding process. The aim of the research was a comparative study of protein spectra of pea interspecific hybrids BC2F5 P. sativum × P. fulvum obtained by the authors and their parents.

Materials and methods. The band composition of seed proteins in the interspecific hybrids of peas BC2F5, variety Stabil (P. sativum) × accession from VIR collection I-609881 (P. fulvum) has been studied. Effectiveness of parent gene transfer determining each polymorphic position of electrophoretic spectrum were evaluated.

Results. The ratio of the actual frequencies of the bands of the cultivated and wild parents in the introgression lines corresponded to the expected level in 73% positions of the electrophoretic spectrum. The introgression rate of individual seed protein bands from wild parent into interspecific pea hybrids in the absence of selection significantly exceeded the expected level, which may indicate the adaptive value of alleles encoding unique seed protein isoforms.

Conclusion. The possibility of introgressive transfer of wild-type alleles to the cultivated genotypes of pea, as well as the presence of identified cultivated isoforms of storage proteins in all studied lines of BC2F5 interspecific hybrids in 88.2% of the polymorphic positions of the electrophoretic spectrum, indicates the possibility of using the wild species P. fulvum in pea breeding.


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About the authors

Sergey V. Bobkov

Federal Scientific Center of Legumes and Groat Crops

Email: svbobkov@gmail.com
ORCID iD: 0000-0002-8146-0791

Russian Federation, Orel

Candidate of Agricultural Sciences, Head of Laboratory, Plant Physiology and Biochemistry Laboratory

Ivan A. Bychkov

Federal Scientific Center of Legumes and Groat Crops

Email: ivan.a.b@mail.ru

Russian Federation, Orel

Junior Scientist, Plant Physiology and Biochemistry Laboratory

Tatyana N. Selikhova

Federal Scientific Center of Legumes and Groat Crops

Email: tat.selihowa@yandex.ru
Orel

Candidate of Biological Sciences, Senior Scientist, Plant Physiology and Biochemistry Laboratory

Elena V. Semenova

Federal Research Center “N.I. Vavilov All-Russian Institute of Plant Genetic Resources”

Author for correspondence.
Email: e.semenova@vir.nw.ru
ORCID iD: 0000-0002-2637-1091

Russian Federation, Saint-Petersburg

Candidate of Biological Sciences, Lead Scientist, Department of Legumes Genetic Resources

Margarita A. Vishnyakova

Federal Research Center “N.I. Vavilov All-Russian Institute of Plant Genetic Resources”

Email: m.vishnyakova.vir@gmail.com
ORCID iD: 0000-0003-2808-7745

Russian Federation, Saint-Petersburg

Doctor of Biological Sciences, Head of Department, Department of Legumes Genetic Resources

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Supplementary files

Supplementary Files Action
1.
Fig. 1. Electrophoretic spectra of protein seeds of the parents of a hybrid combination of Stabil × i-609881 (P. fulvum) and seeds of introgressive pea lines. Introgressive lines: 1 - A1; 2 - A2; 4 - A3; 5 - A4; 6 - A5; 7 - A6; 8 - A7; 9 - A8; 10 - A9; 11 - A10; 12 - A11; 13 - A12; 14 - A13. Parents: 15, 16 - and-609881 (P. fulvum); 17, 18 - variety Stabil. Proteins of soybean seeds of the lanceolate variety are localized in the 3rd spectrum

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2.
Fig. 2. Electrophoretic spectra of protein proteins of the Stabil cultivar: 1–3 — spectra obtained in the presence of mercaptoethanol, 4–9 — spectra without mercaptoethanol, 6 — soy spectrum. In the absence of mercaptoethanol, legumin is localized mainly in the region of 60–65 kDa. In the presence of mercaptoethanol, the legumin molecule dissociates into 2 subunits with molecular weights of 35–46 and 21–23 kDa

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Copyright (c) 2020 Bobkov S.V., Bychkov I.A., Selikhova T.N., Semenova E.V., Vishnyakova M.A.

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