High-throughput sequencing techniques to flax genetics and breeding

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Abstract


Flax (Linum usitatissimum L.) is an important oil and fiber crop. Using modern methods for flax breeding allows accelerating the introduction of some desired genes into the genotypes of future varieties. Today, an important condition for their creation is the development of research, that is based on next-generation sequencing (NGS). This review summarizes the results obtained using NGS in flax research. To date, a linkage map with a high marker density has been obtained for L. usitatissimum, which is already being used for a more efficient search for quantitative traits loci. Comparative studies of transcriptomes and miRNomes of flax under stress and in control conditions elucidated molecular-genetic mechanisms of abiotic and biotic stress responses. The very accurate model for genomic selection of flax resistant to pasmo was constructed. Based on NGS-sequencing also some details of the genus Linum evolution were clarified. The knowledge systematized in the review can be useful for researchers working in flax breeding and whereas fundamental interest for understanding the phylogenetic relationships within the genus Linum, the ontogenesis, and the mechanisms of the response of flax plants to various stress factors.


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About the authors

Alena O. Akhmetshina

Federal Research Center “N.I. Vavilov All-Russian Institute of Plant Genetic Resources”

Author for correspondence.
Email: akhmetshinaalena@gmail.com
ORCID iD: 0000-0002-2346-7258
SPIN-code: 3947-2772

Russian Federation, St. Petersburg

Junior Researcher, Postgenomic Research Department

Ksenia V. Strygina

Federal Research Center “N.I. Vavilov All-Russian Institute of Plant Genetic Resources”

Email: k.strygina@vir.nw.ru
ORCID iD: 0000-0001-6938-1348

Russian Federation, St. Petersburg

PhD, Senior Researcher, Postgenomic research department

Elena K. Khlestkina

Federal Research Center “N.I. Vavilov All-Russian Institute of Plant Genetic Resources”

Email: director@vir.nw.ru
ORCID iD: 0000-0002-8470-8254
SPIN-code: 3061-1429
Scopus Author ID: 6603368411
ResearcherId: T-2734-2017

Russian Federation, St. Petersburg

Prof., Doctor of Science, Director, Head of the Postgenomic Research Department

Elizaveta A. Porokhovinova

Federal Research Center “N.I. Vavilov All-Russian Institute of Plant Genetic Resources”

Email: e.porohovinova@vir.nw.ru
ORCID iD: 0000-0002-8328-9684
SPIN-code: 5033-3263
Scopus Author ID: 22986519000
ResearcherId: S-6756-2016

Russian Federation, St. Petersburg

PhD, Senior Researcher, Oil and Fibre Crops Department

Nina B. Brutch

Federal Research Center “N.I. Vavilov All-Russian Institute of Plant Genetic Resources”

Email: n.brutch@vir.nw.ru
ORCID iD: 0000-0003-2253-6263
SPIN-code: 1753-4382
Scopus Author ID: 26665888600

Russian Federation, St. Petersburg

Doctor of Science, Main Researcher, Oil and Fibre Crops Department

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Supplementary files

Supplementary Files Action
1.
The use of flax DNA markers in studies in different countries. Based on a search for publications in the Scopus database (www.scopus.com, accessed February 1, 2019) by the intersection of the keywords “Marker” and “Linum”

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2.
Fig. 2. Schematic representation of modern breeding approaches based on the use of a combination of approaches for marker-oriented and genomic selection. SNP - single nucleotide polymorphism, DArT - diversity arrays technology, GBS - Genotyping by Sequencing, AFLP - amplified fragment length polymorphism (amplified fragment length polymorphism), SSR - simple sequence repeat (single nucleotide polymorphism), RAPD - random amplification of polymorphic DNA (RFLP - restriction fragment length polymorphism (polymorphism of the length of restriction fragments), QTL - quantitative trait loci (locus of quantitative traits), GWAS - genome -wide association studies (genome-wide Liz associations)

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3.
Fig. 1. The main directions of research flax using DNA markers. The number of publications (numbers in brackets) related to the use of DNA markers in the main areas of flax selection, based on publications in the Scopus database (www.scopus.com, accessed September 13, 2019) at the intersection of the Marker keyword with Fiber Flax ”(flax used for fiber production) /“ Linseed ”(oil flax) and with“ Resistance ”(resistance to biotic stress) /“ Tolerance ”(resistance to abiotic stress) /“ Fiber content ”(fiber content) /“ Seed yield "(seed productivity) /" Fatty acid "(fatty acids). * Not in all articles it is possible to determine which type of flax is involved

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Copyright (c) 2020 Akhmetshina A.O., Strygina K.V., Khlestkina E.K., Porokhovinova E.A., Brutch N.B.

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