Phylogenetic characteristic of nodul bacteria endemic for Southern Ural species of the genus Oxytropis (fabaceae)

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Abstract


Background. An analysis of the spatial distribution of some taxonomically and ecologically related legumes in the Ural showed a nontrivial spatial distribution of related species of the genus Oxytropis DC of the Orobia Bunge section within the Uchalinsky uplands. Despite the similarities in ecology, these species practically do not grow together. Explicit spatial segregation of closely related plants over a relatively small area allows this phenomenon to be used as a convenient model for studying the effect of segregation of closely related legume species on the genetic composition of their nodule bacteria.

Materials and methods. The genetic diversity of nodule bacteria entering into symbiosis with O. kungurensis, O. baschkiriensis, O. approximata and O. gmelinii plants was studied. In addition, the polymorphism of their symbiotic genes has also been analyzed.

Results. Phylogenetic characteristics of nodule bacteria endemic for the Southern Ural belonging to 4 species of leguminous plants of the genus Oxytropis of the section Orobia: O. kungurensis, O. baschkiriensis, O. approximata, O. gmelinii which are characterized by spatial separation of the growth sites, also called plant segregation, are given. It was shown that all of them belong to the genus Mesorhizobium despite certain phylogenetic differences of bacteria. Analysis of the symbiotic genes of the analyzed strains revealed a lack of congruence of their phylogeny with the core part of the genome. It was found that the microsymbionts of O. baschkiriensis plants differ in the phylogeny of nod-genes from nodule bacteria of other plants of the Oxytropis genus and are close to microsymbionts of plants of the Lupinaster genus growing in the Southern Urals.

Conclusion. Acquisition of the property to enter into symbiosis with nodule bacteria of plants of the genus Lupinaster may turn out to be an adaptive mechanism that arose as a result of segregation of O. baschkiriensis from other species of Oxytropis.


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About the authors

Andrei Kh. Baymiev

Institute of Biochemistry and Genetics – Subdivision of the Ufa Federal Research Centre of the Russian Academy of Sciences

Author for correspondence.
Email: baymiev@anrb.ru
ORCID iD: 0000-0001-6637-9365
SPIN-code: 1919-5236
ResearcherId: R-9219-2016

Russian Federation, Ufa

PhD, Leading Researcher, Laboratory of Plant and Microbial Bioengineering

Anastasiya A. Vladimirova

Institute of Biochemistry and Genetics – Subdivision of the Ufa Federal Research Centre of the Russian Academy of Sciences

Email: vladimirovaw@bk.ru
SPIN-code: 2059-9396

Russian Federation, Ufa

Graduate Student, Laboratory of Plant and Microbial Bioengineering

Ekaterina S. Akimova

Institute of Biochemistry and Genetics – Subdivision of the Ufa Federal Research Centre of the Russian Academy of Sciences

Email: iv.katerina-bio@yandex.ru

Russian Federation, Ufa

PhD, Researcher, Laboratory of Plant and Microbial Bioengineering

Roman S. Gumenko

Institute of Biochemistry and Genetics – Subdivision of the Ufa Federal Research Centre of the Russian Academy of Sciences

Email: r.gumenko@yandex.ru
SPIN-code: 4216-4301

Russian Federation, Ufa

Junior Researcher, Laboratory of Plant and Microbial Bioengineering

Albert A. Muldashev

Institute of Biochemistry and Genetics – Subdivision of the Ufa Federal Research Centre of the Russian Academy of Sciences

Email: muldashev_ural@mail.ru
SPIN-code: 1362-7915
Scopus Author ID: 6508160098
ResearcherId: S-3970-2017

Russian Federation, Ufa

PhD, Senior Researcher

Alexei V. Chemeris

Institute of Biochemistry and Genetics – Subdivision of the Ufa Federal Research Centre of the Russian Academy of Sciences

Email: chemeris@anrb.ru
SPIN-code: 1248-2582
Scopus Author ID: 7003329432

Russian Federation, Ufa

PhD, Laboratory of Plant and Microbial Bioengineering

Alexei Kh. Baymiev

Institute of Biochemistry and Genetics – Subdivision of the Ufa Federal Research Centre of the Russian Academy of Sciences

Email: baymiev@mail.ru
ORCID iD: 0000-0003-0606-6740
SPIN-code: 3771-4063
Scopus Author ID: 6507372280
ResearcherId: R-8393-2016

Russian Federation, Ufa

PhD, Head of Laboratory of Plant and Microbial Bioengineering

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Supplementary files

Supplementary Files Action
1.
Fig. 1. Phoregram of RAPD analysis of rhizobia DNA isolated from O. kungurensis nodules. The numbers indicate the numbers of genetically homogeneous groups. M - marker 100 bp

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2.
Fig. 2. Phylogenetic tree of nodule bacteria based on a comparative analysis of the 16S rRNA gene sequences. Strains of microorganisms studied in bold are marked in bold; strains isolated from nodules of L. pentaphyllus and L. albus are underlined

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3.
Fig. 3. The phylogenetic tree of nodule bacteria, based on a comparative analysis of the sequences of the recA gene. Strains of microorganisms studied in bold are marked in bold; strains isolated from nodules of L. pentaphyllus and L. albus are underlined

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4.
Fig. 4. Phylogenetic tree of nodule bacteria, based on a comparative analysis of nifH gene sequences. Strains of microorganisms studied in bold are marked in bold; strains isolated from nodules of L. pentaphyllus and L. albus are underlined

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5.
Fig. 5. Phylogenetic tree of nodule bacteria, based on a comparative analysis of nodC gene sequences. Strains of microorganisms studied in bold are marked in bold; strains isolated from nodules of L. pentaphyllus and L. albus are underlined

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Copyright (c) 2020 Baymiev A.K., Vladimirova A.A., Akimova E.S., Gumenko R.S., Muldashev A.A., Chemeris A.V., Baymiev A.K.

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