Examination of the working environment genotoxic effects on the workers of a coal-fired power plant using the micronucleus test in blood lymphocytes

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Abstract

BACKGROUND: The micronucleus test of peripheral blood lymphocytes provides a qualitative assessment of the state of the genome. A study was carried out aimed at studying the influence of factors of the working environment of coal-fired power plants on the state of the DNA of working data of enterprises.

MATERIALS AND METHODS: Using a micronucleus test with a cytochalasin block on human blood lymphocytes in vitro, 116 men were examined: 45 employees of the Novo-Kemerovo coal-fired power plant and 71 healthy residents of the Kemerovo region.

RESULTS: An increase in the frequency of occurrence of cells with micronuclei, bridges and protrusions in the blood lymphocytes of workers of a coal-fired power plant in comparison with healthy residents of the same area was found.

CONCLUSION: The data confirm the existing idea of the potential danger of combustion products for human health.

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About the authors

Vladislav I. Fedoseev

Federal Research Center of Coal and Coal Chemistry of Siberian Branch of the Russian Academy of Sciences

Author for correspondence.
Email: fedoseev.vlig@gmail.com
ORCID iD: 0000-0002-5359-3845
SPIN-code: 1473-4062

An engineer-technologist of the cytogenetics laboratory

Russian Federation, 18 Sovetski av., 650000 Kemerovo

Danil D. Stepanov

Kemerovo State University

Email: StepanovDanil98@yandex.ru
SPIN-code: 3649-1860

student 

Russian Federation, Kemerovo

Varvara I. Minina

Federal Research Center of Coal and Coal Chemistry of Siberian Branch of the Russian Academy of Sciences; Kemerovo State University

Email: vminina@mail.ru
ORCID iD: 0000-0003-3485-9123
SPIN-code: 5153-8594

Dr. Sci. (Biol.)

Russian Federation, 18 Sovetski av., 650000 Kemerovo; Kemerovo

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Supplementary files

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2. Fig. 1. Frequency of cells with protrusions recorded in groups of smoking and non-smoking workers at thermal power plants. *p = 0.002; hereinafter, the edges of the box indicate 1st and 3rd quartiles (the distance between the 1st and 3rd quartiles is the interquartile range), the line inside the box represents the median; “bars” indicate minimum and maximum values, and dots represent individual values

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3. Fig. 2. Frequency of 3- and 4-nuclear cells in the groups included in the study. * p = 0.001; ** p = 0.0001

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4. Fig. 3. Replication index recorded in the groups included in the study. * p = 0.0001

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5. Fig. 4. Frequency of cells recorded at the mitosis stage for groups included in the study. * p = 0.0001

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6. Fig. 5. Frequency of 1- and 2-nuclear cells observed in individuals included in the control group at different ages. * p = 0.001; ** p = 0.0001

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7. Fig. 6. Frequency of 2-nuclear cells in smokers and non-smokers of the control group. * p = 0,01

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Copyright (c) 2021 Fedoseev V.I., Stepanov D.D., Minina V.I.


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