Studying the genotoxic effects of the working environment on the workers of a coal-fired power plant using a micronuclear test on blood lymphocytes

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Abstract


Background: The micronucleus test of peripheral blood lymphocytes provides a qualitative assessment of the state of the genome. A study was carried out aimed at studying the influence of factors of the working environment of coal-fired power plants on the state of the DNA of working data of enterprises.

Materials and methods: Using a micronucleus test with a cytochalasin block on human blood lymphocytes in vitro, 116 men were examined: 45 employees of the Novo-Kemerovo coal-fired power plant and 71 healthy residents of the Kemerovo region.

Results: An increase in the frequency of occurrence of cells with micronuclei, bridges and protrusions in the blood lymphocytes of workers of a coal-fired power plant in comparison with healthy residents of the same area was found.

Conclusion: The data confirm the existing idea of the potential danger of combustion products for human health.


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About the authors

Vladislav I. Fedoseev

Federal Research Center of Coal and Coal Chemistry of Siberian Branch of the Russian Academy of Sciences

Author for correspondence.
Email: fedoseev.vlig@gmail.com
ORCID iD: 0000-0002-5359-3845
SPIN-code: 1473-4062

Russian Federation, 18 Sovetski av., 650000 Kemerovo

An engineer-technologist of the cytogenetics laboratory

Danil D. Stepanov

Kemerovo State University

Email: StepanovDanil98@yandex.ru
SPIN-code: 3649-1860

Russian Federation, Kemerovo

student 

Varvara I. Minina

Federal Research Center of Coal and Coal Chemistry of Siberian Branch of the Russian Academy of Sciences; Kemerovo State University

Email: vminina@mail.ru
ORCID iD: 0000-0003-3485-9123
SPIN-code: 5153-8594

Russian Federation, 18 Sovetski av., 650000 Kemerovo; Kemerovo

Dr. Sci. (Biol.)

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Supplementary files

Supplementary Files Action
1.
Fig. 1. Frequency of cells with protrusions in groups of smokers and non-smokers at thermal power plants. * p = 0.002; hereinafter, the edges of the box are the 1st and 3rd quartiles (the distance between the 1st and 3rd quartiles is the interquartile time), the line inside the box is the median; "Mustache" - minimal and maximum values, dots are individual values

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2.
Fig. 2. Frequency of 3- and 4-nuclear cells in the studied groups. * p = 0.001; ** p = 0.0001

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3.
Fig. 3. Replication index in the studied groups. * p = 0.0001

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4.
Fig. 4. The frequency of cells at the stage of mitosis in the studied groups. * p = 0.0001

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5.
Fig. 5. Frequency of 1- and 2-nuclear cells in individuals of the control group of different ages. * p = 0.001; ** p = 0.0001

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6.
Fig. 6. Frequency of 2-nuclear cells in smokers and nonsmokers individuals of the control group. * p = 0.01

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