Genome destabilization under stress in cells of the prefrontal cortex, hippocampus and bone marrow of rats with contrast excitability of the nervous system

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Abstract

We studied changes in the stability of the genome in cells of two brain regions (prefrontal cortex and hippocampus), as well as in the bone marrow of rats with a hereditary high and low thresholds of excitability of the nervous system (strains HT and LT, respectively) after prolonged exposure with emotional-pain stressor. To study the reactivity of the brain cells genome, phosphorylated histone γ-H2AX (γ-H2AX phospho Ser139) was used. The level of mitotic disturbances in bone marrow cells was also assessed. Between the animals of the control groups, there were no interstrain differences in the studied parameters. Stress exposure increases the immunoreactivity to γ-H2AX phospho Ser139 of the prefrontal cortex cells and the level of chromosomal aberrations in bone marrow cells in animals of both strains. In cells of the dentate gyrus of the hippocampus, a specific increase in immunoreactivity to γ-H2AX phospho Ser139 was revealed in rats of the low-excitable HT strain. The relationship between the reaction of cells of this zone of hippocampus to the stressor exposure with the hereditary level of excitability of the nervous system of animals is discussed.

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About the authors

Marina B. Pavlova

Pavlov Institute of Physiology, Russian Academy of Sciences

Email: pavlova@infran.ru
SPIN-code: 6457-5630

PhD, Junior Researcher, Laboratory of Higher Nervous Activity Genetics

Russian Federation, Saint Petersburg

Alexander I. Vaido

Pavlov Institute of Physiology, Russian Academy of Sciences

Email: vaidoai@infran.ru
ORCID iD: 0000-0002-6209-9902
SPIN-code: 1323-5153

Dr Sci., Main Researcher, Laboratory of Higher Nervous Activity Genetics

Russian Federation, Saint Petersburg

Diana A.-A. Khlebaeva

Pavlov Institute of Physiology, Russian Academy of Sciences

Email: dibair@yandex.ru

Junior Researcher, Laboratory of Higher Nervous Activity Genetics

Russian Federation, Saint Petersburg

Eugene V. Daev

Saint Petersburg State University

Email: mouse_gene@mail.ru
ORCID iD: 0000-0003-2036-6790

Dr. Sci. (Biol.), Professor

Russian Federation, Saint Petersburg

Natalia A. Dyuzhikova

Pavlov Institute of Physiology, Russian Academy of Sciences

Author for correspondence.
Email: dyuzhikova@infran.ru
ORCID iD: 0000-0002-7550-118X
Scopus Author ID: 6603486439
ResearcherId: J-7202-2018

Dr. Sci. (Biol.), Head of the Laboratory of Higher Nervous Activity Genetics

Russian Federation, Saint Petersburg

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2. Figure: 1. Immunoreactivity to γ-H2AX phospho Ser139 of cells of the medial prefrontal cortex of rats of EP and NP lines 2 h after prolonged emotional pain stress (DEBS) exposure. The graphs show the mean, median, maximum and minimum values, interquartile ranges; * - significant difference from the corresponding control (Mann-Whitney U-test, p <0.05)

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3. Figure: 2. Immunoreactivity to γ-H2AX phospho Ser139 of cells of the dentate gyrus of the hippocampus of EP and NP rats 2 h after prolonged emotional pain stress (DEBS) exposure. The graphs show the mean, median, maximum and minimum values, interquartile ranges; * - significant difference from the corresponding control (Mann-Whitney U-test, p <0.05)

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4. Figure: 3. Frequency of chromosomal aberrations in bone marrow cells of rats of the CAP and NP lines 24 h after prolonged emotional pain stress (DEBS) exposure. The graphs show the mean, median, maximum and minimum values, interquartile ranges; * - the difference from the corresponding control is reliable (Fisher's exact test, p <0.0001)

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5. Figure: 4. Frequency of cells with different types of chromosomal abnormalities in bone marrow cells of rats of the CAP and NP lines 24 h after prolonged emotional painful stress exposure. The graphs show the mean, median, maximum and minimum values, interquartile ranges; the lines indicate reliably different values. The significance of differences between the variants was assessed using Fisher's exact test or χ2 test adjusted for Yates continuity

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Copyright (c) 2021 Pavlova С., Vaido A.I., Khlebaeva K., Daev E.V., Dyuzhikova N.A.

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