Assessment of variability of egg production traits based on analysis of SNP markers and search for traces of selection in the genome of Russian white chickens

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Abstract


Objective. To assess the variability of egg production traits for nine SNPs, to search for traces of selection in the genome of Russian white chickens based on ROH patterns.

Methods. The material for the study was DNA isolated from the blood of Russian white chickens (n = 141). Nine SNPs associated with egg production at p < 5.16 · 10–5 according to GWAS data were selected for analysis. The frequencies of alleles and genotypes, the relationship between genotypes and characteristics of egg production were calculated, and ROH patterns were identified.

Results. Significant differences between genotypes were found in terms of age of laying the first egg (p < 0.005) and egg weight (p < 0.05). The genomic regions surrounding the target SNPs were analyzed according to the distribution of homozygous regions in them.

Conclusions. The substitutions rs317565390 and rs16625488 located in the 4.8–10.2 Mb region on chromosome 8 showed polymorphism, despite the fact that homozygous loci in this region of the genome are found in 58% of animals. For most SNPs, the prevalence of the frequency of one of the alleles was observed. As a cluster of increased selection pressure, a chick genome region in the 4.8–10.2 Mb region on chromosome 8 was identified.


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About the authors

Olga V. Mitrofanova

Russian Research Institute of Farm Animal Genetics and Breeding –
Branch of the L.K. Ernst Federal Science Center for Animal Husbandry

Author for correspondence.
Email: mo1969@mail.ru
ORCID iD: 0000-0003-4702-2736
SPIN-code: 4378-9500
Scopus Author ID: 57188701229
ResearcherId: S-5336-2018

Russian Federation, Pushkin, Saint Petersburg

candidate of biological sciences, scientific secretary

Natalia V. Dementieva

Russian Research Institute of Farm Animal Genetics and Breeding - Branch of the L.K. Ernst Federal Science Center for Animal Husbandry

Email: dementevan@mail.ru
ORCID iD: 0000-0003-0210-9344
SPIN-code: 8768-8906
Scopus Author ID: 57189759592
ResearcherId: Т-4551-2018

Russian Federation, Pushkin, Saint Petersburg

PhD, Main Researcher, Laboratory of Molecular Genetics

Elena S. Fedorova

Russian Research Institute of Farm Animal Genetics and Breeding - Branch of the L.K. Ernst Federal Science Center for Animal Husbandry

Email: Fedorova816@mail.ru
ORCID iD: 0000-0002-1618-6271
SPIN-code: 9226-6133

Russian Federation, Пушкин, Санкт-Петербург

PhD, Senior Researcher, Department of Genetics, Breeding and Conservation of Poultry Genetic Resources

Marina V. Pozovnikova

Russian Research Institute of Farm Animal Genetics and Breeding - Branch of the L.K. Ernst Federal Science Center for Animal Husbandry

Email: pozovnikova@gmail.com
ORCID iD: 0000-0002-8658-2026
SPIN-code: 5441-6996
Scopus Author ID: 57200383317

Russian Federation, Пушкин, Санкт-Петербург

PhD, Senior Researcher, Laboratory of Molecular Genetics

Valentina I. Tyshchenko

Russian Research Institute of Farm Animal Genetics and Breeding - Branch of the L.K. Ernst Federal Science Center for Animal Husbandry

Email: tinatvi@mail.ru
ORCID iD: 0000-0003-4964-9938
SPIN-code: 6294-2400
Scopus Author ID: 35749336500
ResearcherId: K-9523-2017

Russian Federation, Pushkin, Saint Petersburg

PhD, Senior Researcher, Laboratory of Molecular Genetics

Yuriy S. Shcherbakov

Russian Research Institute of Farm Animal Genetics and Breeding - Branch of the L.K. Ernst Federal Science Center for Animal Husbandry

Email: yura.10.08.94.94@mail.ru
ORCID iD: 0000-0001-6434-6287
SPIN-code: 3547-1009
ResearcherId: AAR-5595-2020

Russian Federation, Pushkin, Saint Petersburg

Junior Researcher, Laboratory of Molecular Genetics

Kirill V. Plemyashov

Russian Research Institute of Farm Animal Genetics and Breeding - Branch of the L.K. Ernst Federal Science Center for Animal Husbandry

Email: kirill060674@mail.ru
ORCID iD: 0000-0002-3658-5886
SPIN-code: 4609-8783
Scopus Author ID: 56784261300
ResearcherId: ААО-9837-2020

Russian Federation, Pushkin, Saint Petersburg

Dr Sci., Main Researcher, Reproduction Department

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Copyright (c) 2021 Mitrofanova O., Dementieva N., Fedorova E.S., Pozovnikova M., Tyshchenko V., Scherbakov Y., Plemyashov K.

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