PVYNTN-CP coat protein gene mediated virus resistance of transgenic potato plants



Cite item

Full Text

Abstract

PVYNTN-CP coat protein gene from a necrotic strain of potato virus Y (pvyntnhas been transferred into two potato Solanum tuberosum L. cultivars Mindenes and Somogyi kifli via Agrobacterium tumefaciens transformation. Expression of integrated PVYNTN-CP gene were confirmed for 33 (89 %) of 37 and 3 (75 %) of 4 kanamycin-resistant regenerants of potato cultivars Mindenes and Somogyi kifli respectively. The level of virus resistance against two virus strains (PVY°, PVYNTNof independent lines of transgenic potatoes varied between extreme resistance to susceptibility. The three independent lines of transgenic potatoes proved to be extreme resistant against both PVY strains.

About the authors

Zenon Stasevski

Tatar Research Institute of Agriculture, Kazan, Tatarstan Republic, RF

Email: zenons@bk.ru

Olga N Ilinskaya

Kazan University, Kazan, Tatarstan Republic, RF

Email: Olga.Ilinskaya@ksu.ru

References

  1. Дьяков Ю. Т., Озерецковская О. Л., Джавахия В. Г., Багирова C. Ф., 2001. Общая и молекулярная фитопатология. М.: Общество фитопатологов, 302 с.
  2. Pocc Х., 1989. Селекция картофеля. Проблемы и перспективы. М.: Агропромиздат, 182 с.
  3. Шуберт Й., Pубенштайн Ф., Хрцановска М., Шпаар Д., 2004. К проблеме диагностики штаммов Y-вируса картофеля (PVT)//Вестник защиты растений. Т. 3. С. 3-10.
  4. An G., Watson B. D., Chiang C. C., 1986. Transformation of Tabacco, Tomato, Potato, and Arabidopsis thaliana Using a Binary Ti Vector System//Plant Physiology. Vol. 81. P. 301-305.
  5. Anjum M. A., Ali H., 2004. Effect of Culture Medium on Direct Organogenesis from Different Explants of Various Potato Genotypes//Biotechnology. Vol. 3. P. 187-193.
  6. Angenent G. C., Van Den Ouweland J. M. W., Bol J. F., 1990. Susceptibility to virus infection of transgenic plants expressing structural and non-structural genes of tobacco rattle virus//Virology. Vol. 175. P. 191 -198.
  7. Beachy R. N., 1997. Mechanisms and applications of pathogen-derived resistance in transgenic plants//Current Opinion in Biotechnology. Vol. 8. P. 215-220.
  8. Beczner L., Horvath J., Romhanyi I., Fцrster H., 1984. Studies on the etiology of tuber necrotic ringspot disease in potato//Potato Res. Vol. 27. P. 339-352.
  9. Borque J. E., Miller J. C., Park W. D., 1987. Use of an in vitro tuberization system to study tuber protein gene expression//In Vitro Cellular and Development Biology Vol. 23. P. 381-386.
  10. Clark M. F., Adams A. N., 1977. Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses//J. Gen. Virol. Vol. 34. P. 475-483.
  11. Esna-Ashari M., Villiers T. A., 1998. Plant regeneration from tuber discs of potato (Solanum tuberosum L.) using 6-benzylaminopurine (BAP)//Potato Research. Vol. 41. P. 371-382.
  12. Feher A., Skryabin G. K., Balazs E. et al., 1992. Expression of PVX coat protein gene under the control of extensin-gene promoter confers virus resistance on transgenic potato plants//Plant Cell Report. Vol. 11. P. 48-52.
  13. Gonsalves D., Slightom J. L., 1993. Coat-protein-mediated protection: analysis of transgenic plants for resistance in a variety of crops//Semin. Virol. Vol. 4. P. 397-405.
  14. Hansen J., Nielsen B., Nielsen S. V. S., 1999. In vitro shoot regeneration of Solanum tuberosum cultivars: interactions of medium composition and leaf, leaflet, and explant position//Potato Research. Vol. 42. P. 141 -151.
  15. Harrison B. D., Robinson D. J., 2005. Another quarter century of great progress in understanding the biological properties of plant viruses//Annals of Applied Biology. Vol. 146. P. 15-37.
  16. Hassairi A., Masmoudi K., Albouy J. et al., 1998. Transformation of two potato cultivars Spunta' and Claustar' (Solanum tuberosum) with lettuce mosaic virus coat protein gene and heterologous immunity to potato virus Y//Limerick.: Plant Sci. Vol. 136. P 31-42.
  17. Heeres P., Schippers-Rozenboom M., Jacobsen E., Visser R. G. F., 2002. Transformation of a large number of potato varieties: genotype-dependent variation in efficiency and somaclonal variability//Euphytica. Vol. 124. P. 13-22.
  18. Horvath S., Wolf I., 1999. Virological problems of potato production in Hungary//Abstracts of the 14th Conference of the European Association of Potato Research P. 383-384.
  19. Ishida B. K., Snyder G. W., Belknap W. R., 1989. The use of in vitro grown microtuber disks in Agrobacterium-mediated transformation of Russet Burbank and Lehni Russet potatoes//Plant Cell Report. Vol. 8. P. 325-328.
  20. Jozsa R., Stasevski Z., Balazs E., 2002. High level of field resistance of transgenic tobaccos induced by integrated potato virus Y coat protein gene//Acta Phytopathologica et Entomologica Hungarica. Vol. 37 (4). P. 311-316.
  21. Kollar A., Thole V., Dalmay T., Salamon, P., Balazs E., 1993. Efficient pathogen-derived resistance induced by integrated potato virus Y coat protein gene in tobacco//Biochimie. Vol. 75. P. 623-629.
  22. Lawson C., Kaniewski W., Haley L. et al., 1990. Engineering resistance to mixed virus infection in a commercial potato cultivar: Resistance to potato virus X and potato virus Y in transgenic Russet Burbank//BioTechnology. Vol. 8. P. 127-134.
  23. Miller J. H., 1972. Experiments in molecular genetics. New York.: Cold Spring Harbor Laboratory Press. P. 184-193.
  24. Murashige T., Skoog F., 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures//Physiol Plant. Vol. 15. P. 473-497.
  25. Okamoto D., Nielsen S. V. S., Albrechtsen M., BorkHardt B., 1996. General resistance against potato virus Y introduced into a commercial potato cultivar by genetic transformation with PVYN coat protein gene//Potato Res. Vol. 39. P. 271-282.
  26. Powell-Abel P., Nelson R. S., De B., et al., 1986. Delay of disease development in transgenic plants that express the tobacco mosaic virus coat protein gene//Science. Vol. 232. P. 738-743.
  27. Romano E., Ferreira A. T., Dusi A. N. et al., 2001. Extreme resistance to two Brazilian strains of Potato virus Y (PVY) in transgenic potato, cv. Achat, expressing the PVYO coat protein//Horticultura Brasileira. Vol. 19. P. 118-122.
  28. Rubino L., Carrington J. C., Russo M., 1992. Biologically active cymbidium ringspot virus satellite RNA in transgenic plants suppresses accumulation of DI RNA//Virology. Vol. 188. P. 429-437.
  29. Sambrook J., Frisch E. F., Maniatis T., 1989. Molecular cloning. A laboratory manual. Second edition. New York: Cold Spring Harbor Laboratory Press.
  30. Wittner A., Palkovics L., Balazs E., 1998. Nicotiana benthamiana plants transformed with the plum pox virus helicase gene are resistant to virus infection//Virus Research, Vol. 53. P. 97-103.
  31. Solomon-Blackburn R. M., Barker H., 2001-a. A review of host major-gene resistance to potato viruses X, Y, A and V in potato, genes, genetics and mapped locations//Heredity, Vol. 86. P. 8-16.
  32. Solomon-Blackburn R.M., Barker H., 2001-b. Breeding virus-resistant potatoes (Solanum tuberosum): a review of traditional and molecular approaches//Heredity. Vol. 86. P. 17-35.
  33. Thole V., Dalmay T., Burgyan J., Balazs E., 1993. Cloning and sequencing of potato virus Y (Hungarian isolate) genomic RNA//Gene. Vol. 123. P. 149-156.
  34. Weidemann, H.-L., 1988. Importance and control of potato virus YN (PVYN) in seed potato production//Potato Research. Vol. 31. P. 85-94.
  35. Zambryski P., Joos H., Genetello C. et al., 1983. Ti plasmid vector for the introduction of DNA into plant cells without alteration of their normal regeneration capacity//EMBO J. Vol. 2. P. 2143-2150.

Supplementary files

Supplementary Files
Action
1. JATS XML

Copyright (c) 2009 Stasevski Z., Ilinskaya O.N.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: серия ПИ № ФС 77 - 65617 от 04.05.2016.


This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies