Universal 16s rRNA Primers BD1 For Soil Microbial Community Analysis



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Abstract

New universal 16S rRNa primers were constructed and tested. These primers allow identifying correct taxonomic position of bacterial isolates and were shown to be useful in microbial community studies. The primers enable to detect the vast majority of unique 16S rRNa gene sequences. In the study 160 restriction types were found in 16S rRNa clone library (190 clones).

About the authors

Elisaveta V Korostik

All-Russia Research Institute for Agricultural Microbiology, Saint-Petersburg, RF

Email: korostikliza@yahoo.com

Alexander G Pinaev

All-Russia Research Institute for Agricultural Microbiology, Saint-Petersburg, RF

Email: a_pinaev@yahoo.com

Gulnar A Akhtemova

All-Russia Research Institute for Agricultural Microbiology, Saint-Petersburg, RF

Evgeniy E Andronov

All-Russia Research Institute for Agricultural Microbiology, Saint-Petersburg, RF

Email: eeandr@gmail.com

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Copyright (c) 2006 Korostik E.V., Pinaev A.G., Akhtemova G.A., Andronov E.E.

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