Part 2. Environmental Factors And Individual Features In System Of Evaluation Of Human Genome Instability. Additional Capability Of The Test The Technique For Cytogenetic Analysis



Cite item

Full Text

Abstract

The publication is the 2-nd and the last part of the review analyzing modern trends of the researches in micronuclear test on human blood lymphocytes, cultivated with cytochalasin B. using data of literature and own results the opportunities of application of the test for study association between parameters of genome instability and genetic polymorphism, adaptive response to gamma-irradiation and emotional stress expression are considered. The analysis of additional capability of the test - frequencies of cells with nuclear buds and nucleoplasmic bridge are presents. Finally, results of the international researches on harmonization of the data of the test and guidelines for evaluation genotoxicity of chemical compounds in vitro are described.

About the authors

Faina I Ingel

A.N. Sysin Scientific Research Institute of Human Ecology and Hygien of Environment, Moscow, RF

Email: fainaingel@mail.ru

References

  1. Бондарчук И.А. Анализ роли репарации ДНК, регуляции клеточного цикла и апоптоза в радиоиндуцированном адаптивном ответе клеток млекопитающих Бондарчук И.А.//Радиационная биология и радиоэкология. -2003. -Т. 43, № 1. -С. 19-28.
  2. Ингель Ф.И. Показатели пролиферативной активности и их связь с генетическими повреждениями лимфоцитов крови при культивировании в условиях цитокинетического блока/Ингель Ф.И., Юрченко В.В., Гуськов А.С. [и др.]//Вестник РАМН. -2006. -№ 4. -С. 41-46.
  3. Ингель Ф.И. Качество жизни и индивидуальная чувствительность генома человека. Есть ли выход из порочного круга/Ингель Ф.И.//Экологическая генетика. -2005. -Т. 3, № 3. -С. 38-46.
  4. Ингель Ф.И. Оценка глубины стресса и ее использование при проведении генетико-токсикологических исследований на людях/Ингель Ф.И., Прихожан А.М., Цуцман Т.Е., Ревазова Ю.А.//Вестник Академии медицинских наук. -1997. -№ 7. -С. 24-28.
  5. Ингель Ф.И. Модификация эмоциональным стрессом мутагенных эффектов ксенобиотиков у животных и человека/Ингель Ф.И., Ревазова Ю.А.//Исследования по генетике. Вып. 12. -СПб.: Изд-во С.-Петерб. ун-та, 1999. -С. 86-103.
  6. Мельнов С.Б. Молекулярно-генетический статус подростков, живущих в условиях постоянного воздействия низких доз радиации/Мельнов С.Б., Лебедева Т.Б.//Радиационная биология и радиоэкология. -2004. -Т. 44, № 6. -С. 627-631.
  7. Пелевина И.И. Нестабильность генома после воздействия радиации в малых дозах (в 10-километровой зоне аварии на ЧАЭС и в лабораторных условиях/Пелевина И.И., Готлиб В.Я., Кудряшова О.В.//Радиационная биология и радиоэкология. -1996. -Т. 36, Вып. 4. -С. 546-559.
  8. Пелевина И.И. Радиоиндуцированный адаптивный ответ у детей в зависимости от факторов среды и состояния здоровья/Пелевина И.И., Афанасьев Г.Г., Алещенко А.В. [и др.]//Радиационная биология и радиоэкология. -1999. -Т. 39, № 1. -С. 106-112.
  9. Пелевина И.И. Повышение радиочувствительности после облучения лимфоцитов в адаптивной дозе Пелевина И.И., Алещенко А.В., Готлиб В.Я. [и др.]//Радиационная биология и радиоэкология. -2000. -Т. 40, № 5. -С. 544-548.
  10. Пелевина И.И. Спонтанные и радиоиндуцированные уровни цитогенетических повреждений в лимфоцитах крови детей в зависимости от возраста и качества жизни/Пелевина И.И., Алещенко А.В., Антощина М.М. [и др.]//Радиационная биология и радиоэкология. -2001. -Т. 41, № 5. -С. 573-579.
  11. Селье Г. На уровне целого организма/Селье Г. -Москва: Наука, 1972. -122 с.
  12. Спитковский Д.М. Концепция влияния низких доз ионизирующей радиации на клетки и ее применение для анализа медико-биологических последствий Чернобыльской катастрофы/Спитковский Д.М.//Радиационная биология и радиоэкология. -1992. -Т. 32, Вып. 3. -С. 382-400.
  13. Спитковский Д.М. Теоретический и экспериментальный подходы к проблеме функциональной активности клеток в условиях адаптирующих доз ионизирующего излучения/Спитковский Д.М., Кузьмина И.В.//Радиационная биология и радиоэкология. -2001. -Т. 41, № 5. -С. 599-605.
  14. Спитковский Д.М. Перемещение локусов интерфазных хромосом при действии малых доз радиации Спитковский Д.М., Кузьмина И.В., Макаренков А.С. [и др.]//Радиационная биология и радиоэкология. -2002. -Т. 42, № 6. -С. 604-607.
  15. Худолей В.В. Канцерогены: характеристики, закономерности, механизмы действия/Худолей В.В. -Санкт-Петербург, 1999. -419 с.
  16. Цховребова Л.Б. К механизму адаптивного ответа в клетках человека/Цховребова Л.Б., Македонов Г.П.//Радиационная биология и радиоэкология. -2004. -Т. 44, № 6. -С. 657-661.
  17. Эмоциональный стресс. Физиологические и психологические реакции. Медицинские, индустриальные и военные последствия стресса/Под ред. Л. Леви. -Ленинград: Медицина, 1970. -С. 327.
  18. Abou-Eisha A. Genotoxicity studies on the antimicrobial drug sulfamethoxazole in cultured human lymphocytes Abou-Eisha A., Marcos R., Creus A.//Mutat. Res. -2004. -Vol. 564, N 1. -P. 51-66.
  19. Akudugu J.M. Micronuclei and apoptosis in glioma and neuroblastoma cell lines and role of other lesions in the reconstruction of cellular radiosensitivity/Akudugu J.M., Bohm L.//Radiat Environ Biophys. -2001. -Vol. 40, N 4. -P. 295-300.
  20. Argentin G. Genotoxic and antiapoptotic effect of nicotine on human gingival fibroblasts/Argentin G., Cicchetti R.//Toxicol Sci. -2004. -Vol. 79, N 1. -P. 75-81.
  21. Augustowska K. Polichlorinated biphenyls (РСВ126 and РСВ 153) action on proliferation and progesterone secretion by cultured in vitro porcine luteal cells/Augustowska K., Wojtowicz A., Kajta M. [et al.]//Exp. Clin. Endocrinol. Diabetes. -2001. -Vol. 109, N 8. -P. 416-418.
  22. Barnes P.J. Reactive oxygen species and airway inflammation/Barnes P.J.//Free Rad Biol Med. -1990. -N 9. -P. 235-243.
  23. Barr P.J. Apoptosis and its role in human disease Barr P.J., Tomei L.D.//Biotechnology (N Y). -1994. -Vol. 12, N 5. -P. 487-493.
  24. Bosi A. Variability of the adaptive response to ionizing radiations in humans/Bosi A., Olivieri G.//Mutat. Res. -1989. -Vol. 211, N 1. -P. 13-17.
  25. Bosh K. Evaluation of the toxicological properties of ground-and surface-water samples from the Aral Sea Basin/Bosh K., Erdinger L., Ingel F. [et al.]//Science of the total Environment. -2007. -N 374. -P. 43-50.
  26. Cohen L. DNA repair capacity in healthy medical students during and after exam stress/Cohen L., Marshall G.D. Jr., Cheng L. [et al.]//J. Behav. Med. -2000. -Vol. 23, N 6. -P. 531-544.
  27. Coleman M.A. Low-dose irradiation alters the transcript profiles of human lymphoblastoid cells including genes associated with cytogenetic radioadaptive response Coleman M.A., Yin E., Peterson L.E. [et al.]//Radiat Res. -2005. -Vol. 164, N 4, Pt 1. -P. 369-382.
  28. Darroudi F. Detection of aneugenic and clastogenic potential of X rays, directly and indirectly acting chemicals in human hepatoma (Hep G2) and peripheral blood lymphocytes, using the micronucleus assay and fluorescent in situ hybridization with a DNA centromeric probe/Darroudi F., Meijers C.M., Hadjidekova V., Natarajan A.T.//Mutagenesis. -1996. -Vol. 11, N 5. -P. 425-433.
  29. Dimitroglou E. DNA damage in a human population affected by chronic psychogenic stress/Dimitroglou E., Zafiropoulou M., Messini-Nikolaki N. [et al.]//Int. J. Hyg. Environ. Health. -2003. -Vol. 206, N 1. -P. 39-44.
  30. Donbak L. The genotoxic risk of underground coal miners from Turkey/Donbak L., Rencuzogullari E., Yavuz A., Topaktas M.//Mutat. Res. -2005. -Vol. 588, N 2. -P. 82-87.
  31. Elhajouji A. Metabolic differences between whole blood and isolated lymphocyte cultures for micronucleus (MN) induction by cyclophosphamide and benzo[a]pyrene Elhajouji A., Santos A.P., Van Hummelen P., KirschVolders M.//Mutagenesis. -1994. -Vol. 9, N 4. -P. 307-313.
  32. El-Zein R.A. Cytokinesis blocked micronucleus assay as a novel biomarker for lung cancer risk/El-Zein R.A., Schabath M.B., Etzel C.J. [et al.]//Cancer Res. -2006.-Vol. 66, N 12. -P. 6449-6456.
  33. Erdinger L. The Aral Sea disaster -human biomonitoring of Hg, As, HCB, DDE, aans PCBs in children living in Aralsk and Akchi, Kazakhstan/Erdinger L., Eckl P., Ingel F. [et al.]//International Journal of Hygiene and Environmental Health.-2004.-Vol. 207, N 4.-P. 541-547.
  34. Fenech M. A mathematical model of the in vitro micronucleus assay predicts false negative results if micronuclei are not specifically scored in binucleated cells or in cells that have completed one nuclear division Fenech M.//Mutagenesis. -2000.-Vol.15, N 4.-P. 329-336.
  35. Fenech M. Micronuclei, nucleoplasmic bridges and nuclear buds induced in folic acid deficient human lymphocytes evidence for breakage fusion bridge cycles in the cytokinesis block micronucleus assay/Fenech M., Crott J.W.//Mutat. Res. -2002.-Vol. 504, N 12. -P. 131-136.
  36. Fenech M. Human MicroNucleus project. Intra-and inter-laboratory variation in the scoring of micronuclei and nucleoplasmic bridges in binucleated human lymphocytes. Results of an international slide-scoring exercise by the HUMN project/Fenech M., Bonassi S., Turner J. [et al.]//Mutat Res.-2003.-Vol. 534, N 1-2.-P. 45-64.
  37. Fenech M. Cytokinesis-block micronucleus assay evolves into a "cytome" assay of chromosomal instability, mitotic dysfunction and cell death/Fenech M.//Mut. Res. -2006.-Vol. 600, N 1-2. -P. 58-66.
  38. Fenech M. HUMN project: detailed description of the scoring criteria for the cytokinesis-block micronucleus assay using isolated human lymphocyte cultures Fenech M., Chang W.P., Kirsch-Volders M. [et al.[//Mut. Res.-2003.-Vol. 534.-P. 65-75.
  39. Feng S. Assessing the genotoxicity of imidacloprid and RH-5849 in human peripheral blood lymphocytes in vitro with comet assay and cytogenetic tests/Feng S., Kong Z., Wang X. [et al.]//Ecotoxicol Environ Saf. -2005. -Vol. 61, N 2. -P. 239-246.
  40. Garaj-Vrhovac V. Cytogenetic monitoring of croatian population occupationally exposed to a complex mixture of pesticides/Garaj-Vrhovac V., Zeljezic D.//Toxicology.-2001.-Vol.165, N 2-3.-P. 153-162.
  41. Garcia-Closas M. NAT2 slow acetylation, GSTM1 null genotype, and risk of bladder cancer: results from the Spanish Bladder Cancer Study and meta analyses Garcia-Closas M., Malats N., Silverman D.//Lancet. -2005. -Vol. 366, N 9486. -P. 610-612.
  42. Guo G.Z. Simultaneous evaluation of radiation induced apoptosis and micronuclei in five cell lines/Guo G.Z., Sasai K., Oya N. [et al.]//Int. J. Radiat. Biol. -1998.-Vol. 73, N 3. -P. 297-302.
  43. Guven G.S. Enhanced sensitivity to oxidant-induced micronucleus frequency in elderly individuals is not associated with glutathione S-transferase M1 (GSTM1) null genotype in lymphocytes/Guven G.S., Guven M., Onaran I. [et al.]//Gerontology. -2005. -Vol. 51, N 1. -P. 29-33.
  44. Hayes J.D. Potential contribution of the glutathioneS-transferase supergene family to resistance to oxidative stress/Hayes J.D., Strange R.C.//Free Rad. Res. Commun. -1995. -N 22. -P. 193-207.
  45. Hernandez A. Basal and induced micronucleus frequencies in human lymphocytes with different GST and NAT2 genetic backgrounds/Hernandez A., Xamena N., Gutierrez S. [et al.]//Mutat. Res. -2006. -Vol. 606, N 12. -P. 12-15.
  46. Joksic G. Lack of adaptive response of human lymphocytes exposed in vivo to low doses of ionizing radiation Joksic G., Petrovic S.//J. Environ Pathol. Toxicol. Oncol. -2004. -Vol. 23, N 3. -P. 195-206.
  47. Kalina I. Adaptive response to ionizing radiation in normal and aneuploid human lymphocytes/Kalina I., Konecna H., Nemethova G., Racekova N.//Folia Biol (Praha). -1994. -Vol. 40, N 3. -P. 119-123.
  48. Kirsch-Volders M. Report from the in vitro micronucleus assay working group/Kirsch-Volders M., Sofuni T., Aardema M. [et al.[//Mutat. Res. -2004. -Vol. 564, N 1. -P. 97-100.
  49. Koss L.G. Diagnostic Cytology and its Histopathologic Bases/Koss L.G. -Philadelphia-Toronto: J. B. Lippincott Co, 1979. -Ed 3, Vol. 1, 2.
  50. Leopardi P. Analysis of micronuclei in peripheral blood lymphocytes of traffic wardens: effects of exposure, metabolic genotypes, and inhibition of excision repair in vitro by ARA-C/Leopardi P., Zijno A., Marcon F. [et al.]//Environ Mol. Mutagen. -2003. -Vol. 41, N 2. -P. 126-130.
  51. Maluf S.W. Follow-up study of the genetic damage in lymphocytes of pharmacists and nurses handling antineoplastic drugs evaluated by cytokinesis-block micronuclei analysis and single cell gel electrophoresis assay/Maluf S.W., Erdtmann B.//Mutation Research. -2000. -Vol. 471. -P. 21-27.
  52. Maluf S.W. Assessment of DNA damage in Lymphocytes of Workers Exposed to X-radiation Using the Micronucleus Test and the Comet Assay/Maluf S.W., Ferreira Passos D., Bacelar A. [et al.]//Environmental and Molecular Mutagenesis. -2001. -N 38. -P. 311-315.
  53. Maluf S.W. Monitoring DNA damage following radiation exposure using cytokinesis-block micronucleus method and alkaline single-cell gel electrophoresis/Maluf S.W.//Clinica Chimica Acta. -2004. -N 347. -P. 15-24.
  54. McRobbie S.J. Effects of cytochalasin B on cell movements and chemoattractant elicited actin changes of Dictyostelium/McRobbie S.J., Newell P.C.//Exp. Cell Res. -1985. -Vol. 160, N 2. -P. 275-286.
  55. Migliore L. Preferential occurrence of chromosome 21 malsegregation in peripheral blood lymphocytes of Alzheimer disease patients/Migliore L., Botto N., Scarpato R. [et al.]//Cytogenet Cell Genet. -1999. -Vol. 87, N 1-2. -P. 41-66.
  56. Monsieurs M.A. Adaptive response in patients treated with 131I/Monsieurs M.A., Thierens H.M., Vral A.M. [et al.]//J. Nucl. Med. -2000. -Vol. 41, N 1. -P. 17-22.
  57. Moreno Ramiez E. Study of immunoglobulins, proinflammatory cytokines, lymphoproliferation and phagocytosis in peripheral blood of healthy young people exposed to different levels of atmospheric pollution Moreno Ramiez E., Hernandez Urzua M.A., Gonzalez Villegas A.C. [et al.]//Rev Alerg Mex. -2006. -Vol. 53, N 1. -P. 3-8.
  58. Olivieri G. Adaptive response of human lymphocytes to low concentrations of radioactive thymidine/Olivieri G., Bodycote J., Wolff S.//Science. -1984. -Vol. 223, N 4636. -P. 594-597.
  59. Ossina N.K. Interferon gamma modulates a p53 independent apoptotic pathway and apoptosis related gene expression/Ossina N.K., Cannas A., Powers V.C. [et al.]//J. Biol. Chem. -1997. -Vol. 272, N 6. -P. 351-357.
  60. Pitarque M. Sister chromatid exchanges and micronuclei in peripheral lymphocytes of shoe factory workers exposed to solvents/Pitarque M., Vaglenov A., Nosko M. [et al.]//Environ Health Perspect. -2002. -Vol. 110, N 4. -P. 399-404.
  61. Ponsa I. Non disjunction and chromosome loss in gamma irradiated human lymphocytes: a fluorescence in situ hybridization analysis using centromere specific probes/Ponsa I., Barquinero J.F., Miro R. [et al.]//Radiat. Res. -2001. -Vol. 155, N 3. -P. 424-426.
  62. Psimadas D. DNA damage and repair efficiency in lymphocytes from schizophrenic patients/Psimadas D., Messini-Nikolaki N., Zafiropoulou M. [et al.]//Cancer Lett. -2004. -Vol. 204, N 1. -P. 33-40.
  63. Ridner S. Psychological distress: concept analysis (Nursing theory and concept development or analysis) Ridner S.//J. Advanced Nursing. -2004. -Vol. 45, N 5. -P. 536-545.
  64. Rosefort C. Micronuclei induced by aneugens and clastogens in mononucleate and binucleate cells using the cytokinesis block assay/Rosefort C., Fauth E., Zankl H.//Mutagenesis. -2004. -Vol. 19, N 4. -P. 277-284.
  65. Sakami S. Psychological stress increases human T cell apoptosis in vitro/Sakami S., Nakata A., Yamamura T., Kawamura N.//Neuroimmunomodulation. -2002-2003. -Vol. 10, N 4. -P. 224-231.
  66. Sasaki M.S. DNA damage response pathway in radioadaptive response/Sasaki M.S., Ejima Y., Tachibana A. [et al.]//Mutat. Res. -2002. -Vol. 504, N 1-2. -P.101-118.
  67. Selye H. Thymus and adrenals in the response of the organism to injuries and intoxication/Selye H.//British Journal of Experimental Pathology. -1936. -N 17. -P. 234-248.
  68. Schuler M. Noscapine hydrochloride induced numerical aberrations in cultured human lymphocytes: a comparison of FISH-detection methods and multiple end points/Schuler M., Muehlbauer P., Guzzie P., Eastmond D.A.//Mutagenesis. -2003. -Vol.18, N 3. -P. 235-242.
  69. Silva Mdo C. GSTM1, GSTT1, and GSTP1 genotypes and the genotoxicity of hydroquinone in human lymphocytes/Silva Mdo C., Gaspar J., Duarte Silva I. [et al.]//Environ Mol Mutagen. -2004. -Vol. 43, N 4. -P. 258-264.
  70. Tomei L.D. Psychological stress and phorbol ester inhibition of radiation-induced apoptosis in human peripheral blood leukocytes/Tomei L.D., Kiecolt-laser J.K., Kennedy S., Glaser R.//Psychiatry Res. -1990. -Vol. 33, N 1. -P. 59-71.
  71. Tomei L.D. Aging and apoptosis control/Tomei L.D., Umansky S.R.//Neurol. Clin. -1998. -Vol.16, N 3. -P. 735-745.
  72. Vlastos D. Effects of cetirizine dihydrochloride on human lymphocytes in vitro: micronucleus induction. Evaluation of clastogenic and aneugenic potential using CREST and FISH assays/Vlastos D., Stephanou G.//Arch. Dermatol. Res. -1998. -Vol. 290, N 6. -P. 312-318.
  73. Vodicka P. Cytogenetic Markers, DNA Single-Strand Breaks, Urinary Metabolites, and DNA Repair Rates in Styrene-Exposed Lamination Workers/Vodicka P., Tuimala J., Stetina R. [et al.]//Environmental Health Perspectives. -2004. -Vol. 112, N 8. -P. 867-871.
  74. Wang X. A comparison of folic acid and 5methyltetrahydrofolate for prevention of DNA damage and cell death in human lymphocytes in vitro/Wang X., Fenech M.//Mutagenesis. -2003. -Vol. 18, N 1. -P. 81-86.
  75. Westphal G.A. Thimerosal induces micronuclei in the cytochalasin B block micronucleus test with human lymphocytes/Westphal G.A., Asgari S., Schulz T.G. [et al.]//Arch Toxicol. -2003. -Vol. 77, N 1. -P. 50-55.
  76. Wilkins R.C. Differential apoptotic response to ionizing radiation in subpopulations of human white blood cells Wilkins R.C., Wilkinson D., Maharaj H.P. [et al.]//Mutat Res. -2002. -Vol. 513, N 1-2. -P. 27-36.
  77. Wolff S. Human lymphocytes exposed to low doses of ionizing radiations become refractory to high doses of radiation as well as to chemical mutagens that induce double-strand breaks in DNA/Wolff S., Afzal V., Wiencke J.K. [et al.]//Int. J. Radiat. Biol. Relat. Stud. Phys. Chem Med. -1988. -Vol. 53, N 1. -P. 39-46.
  78. Zeljezic D. Chromosomal aberrations, micronuclei and nuclear buds induced in human lymphocytes by 2,4-dichlorophenoxyacetic acid pesticide formulation Zeljezic D., Garaj-Vrhovac V.//Toxicology. -2004. -N 200. -P. 39-47.
  79. Хусаинова Ш.Н. Оценка состояния здоровья детей Приаралья/Хусаинова Ш.Н., Ингель Ф.И., Петрова И.В. [и др.]//Гигиена и санитария. -2004. -№ 5. -С. 35-38.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2006 Ingel F.I.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: серия ПИ № ФС 77 - 65617 от 04.05.2016.


This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies