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The transformation and genome editing of Pisum sativum: protocols and their modifications
- Авторы: Tvorogova V.E.1, Potsenkovskaia E.A.2, Efremova E.P.1, Kozlov N.V.1, Simonova V.Y.2, Krasnoperova E.Y.1, Konstantinov Z.S.2, Yakovleva D.V.1, Artemiuk A.M.1, Lutova L.A.1
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Учреждения:
- Saint Petersburg State University
- Sirius University
- Выпуск: Том 21 (2023): Спецвыпуск
- Страницы: 32-33
- Раздел: «ГМО: ИСТОРИЯ, ДОСТИЖЕНИЯ, СОЦИАЛЬНЫЕ И ЭКОЛОГИЧЕСКИЕ РИСКИ»
- Статья получена: 15.08.2023
- Статья одобрена: 31.08.2023
- Статья опубликована: 04.12.2023
- URL: https://journals.eco-vector.com/ecolgenet/article/view/567891
- DOI: https://doi.org/10.17816/ecogen567891
- ID: 567891
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Аннотация
Pea (Pisum sativum) is an important agricultural crop and a model object in various fields of plant research. At the same time, the genetic modification of pea is still a difficult task, which, apparently, is associated with its low ability to regenerate. There are a lot of different pea transformation protocols, however, for most of them, the transformation efficiency, i.e. the number of transgenic plants per explant, is extremely low. In addition, none of the protocols known to us has demonstrated itself to be universal, i.e. suitable for all varieties and lines of peas. We searched for studies on the transformation and regeneration of peas and systematized the data obtained. The resulting database made it possible to identify the most effective protocols for the transformation and regeneration of P. sativum, as well as to analyze statistically the general features of the methods used, such as the source of the explant, the composition of the culture media, the duration of cultivation, and so on. We assume that our system for the analysis of publications devoted to in vitro cell cultures can also be used for similar data on other plant species.
This research was supported by the Sirius University of Science and Technology project: PBB-RND-2243.
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Полный текст
Pea (Pisum sativum) is an important agricultural crop and a model object in various fields of plant research. At the same time, the genetic modification of pea is still a difficult task, which, apparently, is associated with its low ability to regenerate. There are a lot of different pea transformation protocols, however, for most of them, the transformation efficiency, i.e. the number of transgenic plants per explant, is extremely low. In addition, none of the protocols known to us has demonstrated itself to be universal, i.e. suitable for all varieties and lines of peas. We searched for studies on the transformation and regeneration of peas and systematized the data obtained. The resulting database made it possible to identify the most effective protocols for the transformation and regeneration of P. sativum, as well as to analyze statistically the general features of the methods used, such as the source of the explant, the composition of the culture media, the duration of cultivation, and so on. We assume that our system for the analysis of publications devoted to in vitro cell cultures can also be used for similar data on other plant species.
This research was supported by the Sirius University of Science and Technology project: PBB-RND-2243.
Об авторах
Varvara Tvorogova
Saint Petersburg State University
Email: krubaza@mail.ru
ORCID iD: 0000-0002-0549-1457
phd, senior researcher, department of genetics and biotechnology
Россия, Saint PetersburgElina Potsenkovskaia
Sirius University
Email: epots556@gmail.com
ORCID iD: 0000-0002-5045-2641
specialist in plant biology and biotechnology
Россия, SochiElena Efremova
Saint Petersburg State University
Email: elenaefr95@gmail.com
ORCID iD: 0000-0002-2565-1155
student in biology, department of genetics and biotechnology
Россия, Saint PetersburgNikolai Kozlov
Saint Petersburg State University
Email: bionkbio@gmail.com
student, department of genetics and biotechnology
Россия, Saint PetersburgVeronika Simonova
Sirius University
Email: nikasimonova14@gmail.com
ORCID iD: 0000-0002-9037-4684
student
Россия, SochiElizaveta Krasnoperova
Saint Petersburg State University
Email: st064840@student.spbu.ru
student in biology, department of genetics and biotechnology
Россия, Saint PetersburgZakhar Konstantinov
Sirius University
Email: zakhar.konstantinov25@gmail.com
ORCID iD: 0000-0002-0825-9418
student
Россия, SochiDaria Yakovleva
Saint Petersburg State University
Email: enginequs@gmail.com
Россия, Saint Petersburg
Anastasiya Artemiuk
Saint Petersburg State University
Email: nastyaart2004@gmail.com
Россия, Saint Petersburg
Ludmila Lutova
Saint Petersburg State University
Автор, ответственный за переписку.
Email: la.lutova@gmail.com
ORCID iD: 0000-0001-6125-0757
dr. sci. (biol.), professor
Россия, Saint PetersburgСписок литературы
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