The MtWOX genes in the regulation of Medicago truncatula somatic embryogenesis
- Authors: Efremova E.P.1, Tvorogova V.E.2, Lutova L.A.1
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Affiliations:
- Saint Petersburg State University
- Saint Petersburg State university
- Issue: Vol 21 (2023): Спецвыпуск
- Pages: 11-11
- Section: Genetically modified organism. The Нistory, Achivements, Social and Environmental Riscs
- URL: https://journals.eco-vector.com/ecolgenet/article/view/568389
- DOI: https://doi.org/10.17816/ecogen568389
- ID: 568389
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Abstract
Plant somatic cells can be reprogrammed into totipotent embryonic cells that are able to form embryos in a process called somatic embryogenesis (SE). SE can occur naturally in some plant species and it is widely used for the plant’s genetic transformation and regeneration.
This process is regulated by hormone treatment and many proteins, among which WUSCHEL-related homeobox (WOX) transcription factors are believed to play crucial roles. Our previous studies have shown that MtWOX9-1 stimulates SE in Medicago truncatula. The aim of the present research was to search for new MtWOX genes regulating SE. In this study, using transcriptomic data and literature data, we had selected several genes with an increased expression level during SE or in the generative organs and examined the overexpression effect of these genes on the SE ability. It was found that explants of the M. truncatula embryogenic line, transformed with the construct for MtWOX6-like overexpression, develop more somatic embryos compared to the control.
Our findings could be a helpful point for searching and studying new morphogenic regulators controlling SE and could have a positive impact on plant biotechnology in improving the transformation and regeneration capacity for other legumes.
This work was supported by the Ministry of Science and Higher Education of the Russian Federation in accordance with agreement No. 075-15-2022-322 date 22.04.2022 on providing a grant in the form of subsidies from the Federal budget of Russian Federation. The grant was provided for state support for the creation and development of a World-class Scientific Center “Agrotechnologies for the Future”.
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Full Text
Plant somatic cells can be reprogrammed into totipotent embryonic cells that are able to form embryos in a process called somatic embryogenesis (SE). SE can occur naturally in some plant species and it is widely used for the plant’s genetic transformation and regeneration.
This process is regulated by hormone treatment and many proteins, among which WUSCHEL-related homeobox (WOX) transcription factors are believed to play crucial roles. Our previous studies have shown that MtWOX9-1 stimulates SE in Medicago truncatula. The aim of the present research was to search for new MtWOX genes regulating SE. In this study, using transcriptomic data and literature data, we had selected several genes with an increased expression level during SE or in the generative organs and examined the overexpression effect of these genes on the SE ability. It was found that explants of the M. truncatula embryogenic line, transformed with the construct for MtWOX6-like overexpression, develop more somatic embryos compared to the control.
Our findings could be a helpful point for searching and studying new morphogenic regulators controlling SE and could have a positive impact on plant biotechnology in improving the transformation and regeneration capacity for other legumes.
This work was supported by the Ministry of Science and Higher Education of the Russian Federation in accordance with agreement No. 075-15-2022-322 date 22.04.2022 on providing a grant in the form of subsidies from the Federal budget of Russian Federation. The grant was provided for state support for the creation and development of a World-class Scientific Center “Agrotechnologies for the Future”.
About the authors
Elena P. Efremova
Saint Petersburg State University
Email: elenaefr95@gmail.com
ORCID iD: 0000-0002-2565-1155
student in biology, department of genetics and biotechnology
Russian Federation, Saint PetersburgVarvara E. Tvorogova
Saint Petersburg State university
Email: v.tvorogova@spbu.ru
ORCID iD: 0000-0002-0549-1457
cand. sci (biol.), senior researcher, department of genetics and biotechnology
Russian Federation, Saint PetersburgLudmila A. Lutova
Saint Petersburg State University
Author for correspondence.
Email: la.lutova@gmail.com
dr. sci. (biol.), professor
Russian Federation, Saint Petersburg