Novel series os pea symbiotic mutants induced in the SGE line



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Abstract

 Using ethylmethansulphonate the chemical mutagenesis of the pea laboratory line SGE was performed. During analysis of 425 families (2069 plants) of М2 progeny 45 putative mutants were selected, among them 30 mutants forming ineffective nodules (Fix– phenotype), 13 mutantsunable to form nodules (Nod– phenotype), and 2 mutants forming a few nodules (Nod+/– phenotype). For 1 Nod– and 5 Fix– mutants monogenic inheritance and recessive phenotype manifestation were demonstrated. For Fix– mutant SGEFix––9 an additional mutation leading to Nod+/– phenotype was shown. Complementation analysis showed that the mutant phenotype of the SGEFix- - 5 line is caused by a mutation in the sym33 gene, of theSGEFix––6 linein the sym40 gene, of the SGEFix––7 line in the sym27 gene, and of the SGEFix––8 linein the sym25 gene.

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About the authors

Viktor E Tsyganov

All-Russia Research Institute for Agricultural Microbiology, Saint-Petersburg, RF

Email: viktor_tsyganov@arriam.spb.ru

Vera A Voroshilova

Contract Drug Development Company “PSI”, St. Petersburg, RF

Email: vera.voroshilova@psi-cro.com

Sergey M Rozov

Institute of Cytology and Genetics SB RAS, Novosibirsk, Novosibirsk Oblast, RF

Email: rozov@bionet.nsc.ru

Aleksey U Borisov

All-Russia Research Institute for Agricultural Microbiology, Saint-Petersburg, RF

Email: ayborisov@yandex.ru

Igor A Tikhonovich

All-Russia Research Institute for Agricultural Microbiology, Saint-Petersburg, RF

Email: arriam@arriam.spb.ru. contact@arriam.spb.ru Podbelskiy Ch., 3, Saint-Petersburg, Pushkin-8

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Copyright (c) 2012 Tsyganov V.E., Voroshilova V.A., Rozov S.M., Borisov A.U., Tikhonovich I.A.

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