Novel series os pea symbiotic mutants induced in the SGE line

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription or Fee Access

Abstract


 Using ethylmethansulphonate the chemical mutagenesis of the pea laboratory line SGE was performed. During analysis of 425 families (2069 plants) of М2 progeny 45 putative mutants were selected, among them 30 mutants forming ineffective nodules (Fix– phenotype), 13 mutantsunable to form nodules (Nod– phenotype), and 2 mutants forming a few nodules (Nod+/– phenotype). For 1 Nod– and 5 Fix– mutants monogenic inheritance and recessive phenotype manifestation were demonstrated. For Fix– mutant SGEFix––9 an additional mutation leading to Nod+/– phenotype was shown. Complementation analysis showed that the mutant phenotype of the SGEFix- - 5 line is caused by a mutation in the sym33 gene, of theSGEFix––6 linein the sym40 gene, of the SGEFix––7 line in the sym27 gene, and of the SGEFix––8 linein the sym25 gene.

Keywords

About the authors

Viktor E Tsyganov

All-Russia Research Institute for Agricultural Microbiology, Saint-Petersburg, RF

Email: viktor_tsyganov@arriam.spb.ru

Vera A Voroshilova

Contract Drug Development Company “PSI”, St. Petersburg, RF

Email: vera.voroshilova@psi-cro.com

Sergey M Rozov

Institute of Cytology and Genetics SB RAS, Novosibirsk, Novosibirsk Oblast, RF

Email: rozov@bionet.nsc.ru

Aleksey U Borisov

All-Russia Research Institute for Agricultural Microbiology, Saint-Petersburg, RF

Email: ayborisov@yandex.ru

Igor A Tikhonovich

All-Russia Research Institute for Agricultural Microbiology, Saint-Petersburg, RF

Email: arriam@arriam.spb.ru. contact@arriam.spb.ru Podbelskiy Ch., 3, Saint-Petersburg, Pushkin-8

References

  1. Борисов А. Ю., Розов С. М., Цыганов В. Е. и др., 1994. Выявление симбиотических генов гороха (Pisum sativum L.) с использованием экспериментального мутагенеза//Генетика. Т 30. C. 1484-1494.
  2. Борисов А. Ю., Штарк О. Ю., Жуков А. В. и др., 2011. Взаимодействие бобовых с полезными почвенными микроорганизмами: от генов растений к сортам//С/х биология. № 3. С. 41-47.
  3. Борисов А. Ю., Проворов Н. А., Тихонович И. А., Цыганов В. Е., 1998. Генетический контроль взаимодействия бобовых растений с клубеньковыми бактериями//Генетика симбиотической азотфиксации с основами селекции/Под ред. Тихоновича И. А. и Проворова Н. А. Санкт-Петербург: Наука. C. 8-62.
  4. Barker D., Bianchi S., Blondon F. et al., 1990. Medicago truncatula, a model plant for studying the molecular genetics of the Rhizobium-legume symbiosis//Plant. Mol. Biol. Rep. Vol. 8 P. 40-49.
  5. Bhatia C. R., Nichterlein K., Maluszynski M., 2001. Mutations affecting nodulation in grain legumes and their potential in sustainable cropping system//Euphytica. Vol. 120. P. 415-432.
  6. Borisov A. Y., Morzhina E. V., Kulikova O. A. et al., 1992. New symbiotic mutants of pea (Pisum sativum L.) affecting either nodule initiation or symbiosome development//Symbiosis. Vol. 14. P. 297-313.
  7. Borisov A. Y., Danilova T. N., Koroleva T. A. et al., 2004. Pea (Pisum sativum L.) regulatory genes controlling development of nitrogen-fixing nodule and arbuscular mycorrhiza: fundamentals and application Biologia. Vol. 59/Suppl. P. 137-144.
  8. Duc G., Messager A., 1989. Mutagenesis of pea (Pisum sativum L.) and the isolation of mutants for nodulation and nitrogen fixation//Plant Sci. Vol. 60. P. 207-213.
  9. Engvild K. J., 1987. Nodulation and nitrogen fixation mutants of pea (Pisum sativum)//Theor. Appl. Genet. Vol. 74. P. 711-713.
  10. Handberg K., Stougaard J., 1992. Lotus japonicus, an autogamous, diploid legume species for classical and molecular genetics//Plant J. Vol. 2. P. 487-496.
  11. Jacobsen E., 1984. Modification of symbiotic interaction of pea (Pisum sativum L.) and Rhizobium leguminosarum by induced mutations//Plant Soil. Vol. 82. P. 427-438.
  12. Kneen B. E., LaRue T. A., 1988. Induced symbiosis mutants of pea (Pisum sativum) and sweetclover (Melilotus alba annua)//Plant Sci. Vol. 58. P. 177-182.
  13. Kneen B. E., LaRue T. A., Hirsch A. M., et al., 1990. sym-13 -A gene conditioning ineffective nodulation in Pisum sativum.//Plant Physiol. Vol. 94. P. 899-905.
  14. Kosterin O. E., Rozov S. M., 1993. Mapping of the new mutation blb and the problem of integrity of linkage group I//Pisum Genet. Vol. 25. P. 27-31.
  15. Kouchi H., Imaizumi-Anraku H., Hayashi M., et al., 2010. How many peas in pod? Legume genes responsible for mutualistic symbioses underground//Plant Cell Physiol. Vol. 51. P. 1381-1397.
  16. Novák K., 2003. Allelic relationships of pea nodulation mutants//J. Hered. Vol. 94. P. 191-193.
  17. Oldroyd G. E. D., Downie J. A., 2004. Calcium, kinases and nodulation signalling in legumes//Mol. Cell Biol. Vol. 5. P. 566-576.
  18. Safronova V. I., Novikova N. I., 1996. Comparison of two methods for root nodule bacteria preservation: Lyophilization and liquid nitrogen freezing//J. Microbiol. Methods. Vol. 24. P. 231-237.
  19. Sagan M., Duc G., 1996. Sym28 and Sym29, two new genes involved in regulation of nodulation in pea (Pisum sativum L.)//Symbiosis. Vol. 20. P. 229-245.
  20. Sagan M., Huguet T., Barker D., Duc G., 1993. Characterization of two classes of non-fixing mutants of pea (Pisum sativum L.)//Plant Sci. Vol. 95. P. 55-66.
  21. Sagan M., Huguet T., Duc G., 1994. Phenotypic characterization and classification of nodulation mutants of pea (Pisum sativum L.). Plant Sci. Vol. 100. P. 59-70.
  22. Schauser L., Roussis A., Stiller J., Stougaard J., 1999. A plant regulator controlling development of symbiotic root nodules//Nature. Vol. 402. P. 191-195.
  23. Stacey G., Libault M., Brechenmacher L. et al., 2006. Genetics and functional genomics of legume nodulation//Curr. Opin. Plant Biol. Vol. 9. P. 110-121.
  24. Stougaard J., 2001. Genetics and genomics of root symbiosis//Curr. Opin. Plant Biol. Vol. 4. P. 328-335.
  25. Szczyglowski K., Shaw R. S., Wopereis J. et al., 1998. Nodule organogenesis and symbiotic mutants of the model legume Lotus japonicus//Mol. Plant-Microbe Interact. Vol. 11. P. 684-697.
  26. Tsyganov V. E., Borisov A. Y., Rozov S. M., Tikhonovich I. A., 1994. New symbiotic mutants of pea obtained after mutagenesis of laboratory line SGE//Pisum Genetics. Vol. 26. P. 36-37.
  27. Tsyganov V. E., Morzhina E. V., Stefanov S. Y., et al., 1998. New pea (Pisum sativum L.) genes sym33 and sym40 control infection thread formation and root nodule function//Mol. Gen. Genet. Vol. 256. P. 491-503.
  28. Tsyganov, V. E., Borisov A. Y., Tikhonovich I. A., 2001. Fix-mutants RisFixA and RisFixV carry mutations in newly identified pea (Pisum sativum L.) genes sym41 and sym42, respectively//Pisum Genet. Vol. 33. P. 36.
  29. Udvardi M. K., 2001. Legume models strut their stuff//Mol. Plant-Microbe Inter. Vol. 14. P. 6-9.

Statistics

Views

Abstract - 443

PDF (Russian) - 332

Cited-By


Article Metrics

Metrics Loading ...

PlumX

Dimensions


Copyright (c) 2012 Tsyganov V.E., Voroshilova V.A., Rozov S.M., Borisov A.U., Tikhonovich I.A.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies