GENETIC AND MORPHOTYPIC HETEROGENEITY OF SWIMMING POOL BACTERIAL POPULATIONS



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Abstract

The populations of resident bacterial species of the swimming pool community such as Pseudomonas aeruginosa, Bacillus sp., Acinetobacter lwoffii and Pseudomonas alcaligenes were analyzed. All these species showed stable in time heterogeneity by spontaneous mutation frequency and biofilm forming ability. There was notably high occurrence of mutators in all investigated populations. Our results show high level of genetic plasticity and adaptivity under conditions of starvation and exposure to biocides. 

About the authors

Larisa A Magdanova

Institute of ecology and enetics of microorganisms UB RAS, Perm, Perm Krai, RF

Email: lakonic@rambler.ru

Nadezhda V Golyasnaya

Institute of ecology and enetics of microorganisms UB RAS, Perm, Perm Krai, RF

Email: goljasnaja@mail.ru

References

  1. Беркли Р. и др., 1997. Определитель бактерий Берд-жи. В 2 т. Т.1. Пер. с англ./Под ред. Дж. Хоулта и др. М.: Мир, 432 с.
  2. Беркли Р. и др., 1997. Определитель бактерий Берд-жи. В 2 т. Т. 2. Пер. с англ./Под ред. Дж. Хоулта и др. М.: Мир, 368 с.
  3. Герхардт Ф., 1984. Методы общей бактериологии. В 3 т. T. 1. Пер. с англ./Под ред. Ф. Герхардта и др. М.: Мир, 264 с.
  4. Маниатис Т. И., Фрич Э., Сэмбрук Дж., 1984. Методы генетической инженерии. Молекулярное клонирование/Пер. с англ. М.: Мир, 480 с.
  5. Atlas R. M., 2004. Handbook of Microbiological Media. 3nd ed./Eds. R. M. Atlas. London: CRC Press, 2051 p.
  6. Baquero M. R., Nilsson A. I., Turrientes M. C., 2004. Polymorphic mutation frequencies in Escherichia coli: emergence of weak mutators in clinical isolates//J. Bacteriol., Vol. 186. N 16. P. 5538-5542.
  7. Bjarnsholt T., Jensen P. Ø., Burmolle M. et al., 2005. Pseudomonas aeruginosa tolerance to tobramycin, hydrogen peroxide and polymorphonuclear leukocytes is quorum-sensing dependent//Microbiology. Vol. 151. P. 373-383.
  8. Bjarnsholt T., Jensen P. Ø., Jakobsen T. H. et al., 2010. Quorum sensing and virulence of Pseudomonas aeruginosa during lung infection of cystic fibrosis patients//PLoS One. Vol. 5. N 4. e10115.
  9. Boles B. R., Thoendel M., Singh P. K. Self-generated diversity produces "insurance effects" in biofilm communities//Appl. Environ. Microbiol. Vol. 71. N 3. P. 1163-1168.
  10. Caiazza N. C., Shanks R. M. Q., O'Toole G. A., 2005. Rhamnolipids Modulate Swarming Motility Patterns of Pseudomonas aeruginosa//J. Bacteriol., Vol. 187. N 21. P. 7351-7361.
  11. Cornelis P., 2008. Pseudomonas: genomics and molecular biology. Horizon Scientific Press. 244 p.
  12. Garrity G. M., 2005. Bergey's Manual of Systematic Bacteriology. 2nd ed. Vol. 2./Eds. D. J. Brenner et al. New York: Springer, 1388 p.
  13. Kenna D. T., Doherty C. J., Foweraker J. et al., 2007. Hypermutability in environmental Pseudomonas aeruginosa and in populations causing pulmonary infection in individuals with cystic fibrosis//Microbiology. Vol. 153. P. 1852-1859.
  14. Lappin-Scott H. M., Costerton W. J., 2003. Microbial Biofilms. 2nd ed./Eds. H. M. Lappin-Scott, W. J. Costerton. Cambridge University Press, 328 p.
  15. Mena A., Smith E. E., Burns J. L. et al., 2008. Genetic adaptation of Pseudomonas aeruginosa to the airways of cystic fibrosis patients is catalyzed by hypermutation//J. Bacteriol. Vol. 190. N 24. P. 7910-7917.
  16. Merritt J. H. Kadouri D. E., O'Toole G. A., 2005. Growing and analyzing static biofilms//Current Protocols in Microbiology. 2nd ed./Eds. R. Coico. New York: John Wiley & Sons, Inc., 1B.1.1-1B.1.17.
  17. Oliver A., Baquero F., Blázquez J., 2002. The mismatch repair system (mutS, mutL and uvrD genes) in Pseudomonas aeruginosa: molecular charachterization of naturally occurring mutants//Mol. Microbiol., Vol. 43. N 6. P. 1641-1650.
  18. Shehata T. E., Duran A., Collins E. B., 1971. Influence of temperature on the growth of psychrophilic strains of Bacillus//J. Dairy Sci. Vol. 54. N 11. P. 1579-1582.
  19. Siegmund I., Wagner F., 1991. New method for detecting rhamnolipids excreted by Pseudomonas species during growth on mineral agar//Biotechnol. Tech. Vol. 5. N 4. P. 265-268.
  20. Sikorski J., Nevo E., 2005. Adaptation and incipient sympatric speciation of Bacillus simplex under microclimatic contrast at "Evolution Canyons" I and II, Israel//PNAS. Vol. 102. N 44. P. 15924-15929.
  21. Sniegowski P. D., Gerrish P. J., Lenski R. E., 1997. Evolution of high mutation rates in experimental populations of E. coli//Nature. N 387. P. 703-705.
  22. Taddei F., Radman M., Maynard-Smith J. et al., 1997. Role of mutator alleles in adaptive evolution//Nature. N 19. P.700-702.
  23. Watts J. L., Shryock T. R., Apley M. et al. Performance standards for antimicrobial susceptibility tests for bacteria isolated from animals; Approved standard. 3rd ed.//Wayne, Pennsylvania: Clinical and Laboratory Standard Institute, CLSI document M31-A3.
  24. Webb J. S., Lau M., Kjelleberg S., 2004. Bacteriophage and phenotypic variation in Pseudomonas aeruginosa biofilm development//J. Bacteriol. Vol. 186. N 23. P. 8066-8073.
  25. Zgur-Bertok D. Phenotypic heterogeneity in bacterial populations//Acta Agric. Slovenica. 2007. Vol. 90. N 1. P. 17-24.

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