The ITS1-5.8S rRNA gene -ITS2 sequence variability during the divergence of sweet-grass species (gen us Glyceria R. Br.)

Abstract


Comparative analysis of the sequence ITS1-5.8S rRNA gene-ITS2 of the nuclear genome of 13 species of genus Glyceria, 4 species of Melica and a species of monotypic genus Pleuropogon showed that the species of the genus Glyceria have 3 haplotypes: 1) Haplotype A was found only in species of the subgenus Glyceria section Glyceria (G. septentrionalis, G. fluitans, G. declinata, G. occidentalis, G. notata, G. borealis, G. leptostachya) and in Pleuropogon sabinii; 2) Haplotype C is characteristic of the subgenus Hydropoa, section Hydropoa (G. grandis, G. х amurensis, G. triflora, G. maxima) and sect. Lithuanicae (G. leptolepis); 3) Haplotype B is found in the species of the subgenus Hydropoa sections Striatae (G. elata, G. striata, G. neogaea, G. canadensis), Scolochloiformes (G. alnasteretum, G. spiculosa) and G. lithuanica of sect. Lithuanicae. Species carring haplotype B are located at the base of the phylogenetic tree of the genus Glyceria and/or clustered with low bootstrap indices. On the phylogenetic trees inferred by the analysis of the sequences ITS and 5.8S rDNA both sect. Glyceria and sect. Hydropoa represented two sister monophyly branches. The species Pleuropogon sabinii belong to the branch of subgenus Glyceria as a sister monotypic branch to the branch of the sect. Glyceria.

Keywords



Alexander V Rodionov

V. L. Komarov Botanical Institute RAS, Saint-Petersburg, RF

Email: avrodionov@mail.ru

Armen R Kotsinyan

V. L. Komarov Botanical Institute RAS, Saint-Petersburg, RF

Email: avrodionov@mail.ru Professora Popova st., 2., St.Petersburg, 197376, Russia

Alexander A Gnutikov

V. L. Komarov Botanical Institute RAS, Saint-Petersburg, RF

Email: avrodionov@mail.ru Professora Popova st., 2., St.Petersburg, 197376, Russia

Marina A Dobroradova

Saint-Petersburg State University., Saint-Petersburg, RF

Email: avrodionov@mail.ru 7-9, Universitetskaya nab., St.Petersburg, 199034, Russia

Eduard M Machs

V. L. Komarov Botanical Institute RAS, Saint-Petersburg, RF

Email: Professora Popova st., 2., St.Petersburg, 197376, Russia

  1. Ким Е. С., Большева Н. Л., Саматадзе Т. Е. и др., 2009. Уникальный геном двухромосомных злаков Zingeria и Colpodium, его происхождение и эволюция // Генетика. Т. 45. С. 1506-1515.
  2. Комаров В. Л., 1934. Род 176. Манник - Glyceria R. Br. // Флора СССР. Т. 2 / Ред. В. Л. Комаров, Ленинград: Издательство Академии наук СССР. С. 449- 460.
  3. Родионов А. В., Ким Е. С., Носов Н. Н. и др., 2008. Молекулярно-филогенетическое исследование видов рода Colpodium sensu lato (Poeae, Poaceae) // Экологическая генетика. Т. 6. № 4. С. 34-46.
  4. Родионов А. В., Тюпа Н. Б., Ким Е. С. и др., 2005. Геномная конституция автотетраплоидного овса Avena macrostachya, выявленная путем сравнительного анализа последовательностей ITS1 и ITS2: к вопросу об эволюции кариотипов овсов и овсюгов на ранних этапах дивергенции видов рода Avena // Генетика. Т. 41. C. 646-656.
  5. Цвелев Н. Н., 2006. Краткий обзор рода манник Glyceria (Poaceae) // Бот. журн. Т. 91. С. 255-276.
  6. Шнеер В. С., 2009. ДНК штрихкодирование - новое направление в сравнительной геномике растений // Генетика. Т. 54. С. 1436-1448.
  7. Alvarez A. E., Wendel J. F., 2003. Ribosomal ITS sequences and plant phylogenetic inference // Molec. Phylogenet. Evol. Vol. 29. P. 417-434.
  8. Calonje M., Martin-Bravo S., Dobeš C. et al., 2009. Non-coding nuclear DNA markers in phylogenetic reconstruction // Plant Syst. Evol. Vol. 282. P. 257-280.
  9. Charch G. L., 1949. A cytotaxonomic study of Glyceria and Puccinellia// Am. J. Bot. Vol. 36. P. 155- 165.
  10. Coleman A. W., 2009. Is there a molecular key to the level of "biological species" in eukaryotes? A DNA guide // Molec. Phylogenet. Evol. Vol. 50. P. 197- 203.
  11. Gillespie L. J., Archambault A., Soreng R. J., 2007. Phylogeny of Poa (Poaceae) based on trnT-trnP sequence data: major clades and basal relationships // Monocots: comparative biology and evolution-Poales. /Eds. J. T. Columbus, E. A. Friar, J. M. Porter et al. Claremont, Calif.: Rancho Santa Ana Botanic Garden. P. 420-434.
  12. Koš M., Tollervey D., 2010. Yeast pre-rRNA processing and modification occur cotranscriptionally // Mol. Cell. Vol. 37. P. 809-820.
  13. Mejia-Saules T., Bisby F., 2000. A. preliminary views on the tribe Meliceae (Gramineae: Pooideae) // Grasses: systematics and evolution / Eds. S. W. L. Jacobs, J. Everett. Collingwood, VIC, Australia: CSIRO Publishing. P. 83-88.
  14. Nieto Feliner G., Rosello J. A., 2007. Better the devil you know? Guidelines for insightful utilization of nrDNA ITS in species-level evolutionary studies in plants // Molec. Phylogenet. Evol. Vol. 44. P. 911-919.
  15. Ridgway K. P., Duck J. M., Young J. P. W., 2003. Identification of roots from grass swards using PCR RFLP and FFLP of the plastid trnL (UAA) intron // BMC Ecol. Vol. 3. P. 8.
  16. Schneider J., Doring E., Hilu K. W., Roser M. 2009. Phylogenetic structure of the grass subfamily Pooideae based on comparison of plastid matK gene-3' trnK exon and nuclear ITS sequences // Taxon. Vol. 58. P. 405- 424.
  17. Tamura K., Dudley J., Nei M., Kumar S., 2007. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) soft ware version 4.0 // Mol. Biol. Evol. Vol. 24. P. 1596- 1599.
  18. Tzvelev N. N., 1989. The system of Grasses (Poaceae) and their evolution // Botanical Review. Vol. 55. P. 141- 203.
  19. Whipple I. G., Barkworth M. E., Bushman B. S., 2007. Molecular insights into the taxonomy of Glyceria (Poaceae: Meliceae) in North America // Am. J. Bot. Vol. 94. P. 551-557.
  20. White T. J., Bruns T., Lee S., Taylor J., 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics // PCR Protocols: a Guide to Methods and Applications / Eds M. A. Innis, D. H. Gelfand,J. J. Sninsky, T. J. White. San Diego: Academic Press Inc. P. 315-322.

Views

Abstract - 267

PDF (Russian) - 314

Cited-By


PlumX


Copyright (c) 2011 Rodionov A.V., Kotsinyan A.R., Gnutikov A.A., Dobroradova M.A., Machs E.M.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies