Bacterial genera associated with nitrogen cycle in microbial communities of chernozems



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Abstract

Background: Reactions associated with the geochemical nitrogen cycle occurring in the soil have a decisive effect on the level of nitrogen availability for green plants. Microorganisms that catalize them are of interest from the point of view of developing mechanisms for managing soil fertility. In this paper, we focused on bacterial genera associated with nitrogen cycle processes in chernozem soils of the Belgorod region of Russia.

Materials and methods: Samples of arable and non-arable chernozems of three subtypes were collected in June and August 2022. Microbiological profiling based on high-throughput sequencing of amplicons of V3-V4 region of the 16S rRNA gene with subsequent computational processing of the results was used in the work.

Results: 6 genera of nitrogen-fixing bacteria were found, as well as a group undifferentiated by means of the method used, including genus Rhizobium. 8 genera involved in the first and 5 genera involved in the second stage of nitrification were also found. 7 genera have been found whose representatives are capable of denitrification, without taking into account nitrogen-fixing bacteria, which are also capable of carrying out this process.

Conclusions: Among the bacteria capable of fixing atmospheric nitrogen in the chernozems of the Belgorod region, the genus Bradyrhizobium dominates. Among the nitrifiers performing the first stage of nitrification, Ellin6067 and MND1 from the family Nitrosomonadaceae predominate. The second stage of nitrification is carried out mainly by bacteria of the genus Nitrospira. Of the potential denitrifiers, along with nitrogen-fixing genera possessing this ability, representatives of the genera Rubrobacter and Pseudomonas are the most numerous. The study does not claim to give a complete list of organisms that carry out key chemical transformations of nitrogen compounds in chernozems, however, it allows us to name their important participants.

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About the authors

K. S. Boyarshin

Belgorod State University (BelSU)

Email: kboyarshin@mail.ru
ORCID iD: 0000-0002-2960-0670
SPIN-code: 6002-9327
Scopus Author ID: 57218822292
ResearcherId: HJZ-5162-2023

Senior Lecturer at the Department of Biotechnology and Microbiology

Russian Federation, 85 Pobedy str., Belgorod, Russia, 308015

V. V. Adamova

Belgorod State University (BelSU)

Email: adamova@bsu.edu.ru
ORCID iD: 0000-0001-8329-4670

Docent at the Department of Biology

Russian Federation, 85 Pobedy str., Belgorod, Russia, 308015

Wentao Zheng

Belgorod State University (BelSU)

Email: zhengwentaoo@126.com
ORCID iD: 0009-0003-3460-8401

Graduate student at the Department of Biotechnology and Microbiology

Russian Federation, 85 Pobedy str., Belgorod, Russia, 308015

E. V. Nikitinskaya

Cherepovets State University (ChGU)

Email: nikitinskajacat@yandex.ru
Russian Federation, 5 Lunacharskogo Ave., Cherepovets, Russia, 162600

O. Yu. Obukhova

Belgorod State University (BelSU)

Email: 1064261@bsu.edu.ru
ORCID iD: 0009-0007-5139-0394

Graduate student at the Department of Biotechnology and Microbiology

Russian Federation, 85 Pobedy str., Belgorod, Russia, 308015

M. V. Kolkova

Belgorod State University (BelSU)

Email: mvk3105@mail.ru
ORCID iD: 0009-0008-3849-3564

Student at the Department of Biotechnology and Microbiology

Russian Federation, 85 Pobedy str., Belgorod, Russia, 308015

O. S. Bespalova

Belgorod State University (BelSU)

Email: olga9078@mail.ru

Student at the Department of Biotechnology and Microbiology

Russian Federation, 85 Pobedy str., Belgorod, Russia, 308015

V. V. Kluyeva

Belgorod State University (BelSU)

Email: klyueva@bsu.edu.ru
ORCID iD: 0000-0002-9509-5115

Senior Lecturer at the Department of Biotechnology and Microbiology

Russian Federation, 85 Pobedy str., Belgorod, Russia, 308015

K. A. Degtyareva

Senior Lecturer at the Department of Biotechnology and Microbiology

Email: degtyareva@bsu.edu.ru
ORCID iD: 0000-0003-4474-0919

Senior Lecturer at the Department of Biotechnology and Microbiology

Russian Federation, 85 Pobedy str., Belgorod, Russia, 308015

L. V. Nesteruk

Belgorod State University (BelSU)

Email: nesteruk@bsu.edu.ru
ORCID iD: 0000-0003-3189-8178

Senior Lecturer at the Department of Biotechnology and Microbiology

Russian Federation, 85 Pobedy str., Belgorod, Russia, 308015

Yu. N. Kurkina

Belgorod State University (BelSU)

Email: kurkina@bsu.edu.ru
ORCID iD: 0000-0001-9180-1257

Docent at the Department of Biotechnology and Microbiology

Russian Federation, 85 Pobedy str., Belgorod, Russia, 308015

O. A. Makanina

Belgorod State University (BelSU)

Email: makanina@bsu.edu.ru
ORCID iD: 0009-0006-7571-2493

Docent at the Department of Biotechnology and Microbiology

Russian Federation, 85 Pobedy str., Belgorod, Russia, 308015

E. S. Ivanova

Cherepovets State University (ChGU)

Email: stepinaelena@yandex.ru
ORCID iD: 0000-0002-6976-1452

Head of the Ecological and Analytical Laboratory

Russian Federation, 5 Lunacharskogo Ave., Cherepovets, Russia, 162600

I. V. Batlutskaya

Belgorod State University (BelSU)

Author for correspondence.
Email: bat@bsu.edu.ru
ORCID iD: 0000-0003-0068-6586

Head of the Department of Biotechnology and Microbiology

Russian Federation, 85 Pobedy str., Belgorod, Russia, 308015

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